Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABLV49_RS01415 Genome accession   NZ_CP157675
Coordinates   274837..275973 (-) Length   378 a.a.
NCBI ID   WP_349279887.1    Uniprot ID   -
Organism   Polaromonas hydrogenivorans strain DSM 17735     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269837..280973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLV49_RS01385 (ABLV49_01385) - 269869..271095 (-) 1227 WP_349279883.1 multidrug effflux MFS transporter -
  ABLV49_RS01390 (ABLV49_01390) rsmI 271092..271952 (-) 861 WP_349281558.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ABLV49_RS01395 (ABLV49_01395) - 272003..272464 (+) 462 WP_349279884.1 YraN family protein -
  ABLV49_RS01400 (ABLV49_01400) - 272589..273188 (+) 600 WP_011799705.1 phosphoheptose isomerase -
  ABLV49_RS01405 (ABLV49_01405) - 273185..273850 (+) 666 WP_349279885.1 BON domain-containing protein -
  ABLV49_RS01410 (ABLV49_01410) - 273903..274805 (-) 903 WP_349279886.1 NAD(P)-dependent oxidoreductase -
  ABLV49_RS01415 (ABLV49_01415) pilU 274837..275973 (-) 1137 WP_349279887.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABLV49_RS01420 (ABLV49_01420) - 276021..276653 (-) 633 WP_349279888.1 cyclic nucleotide-binding domain-containing protein -
  ABLV49_RS01425 (ABLV49_01425) pilT 276686..277729 (-) 1044 WP_011799709.1 type IV pilus twitching motility protein PilT Machinery gene
  ABLV49_RS01430 (ABLV49_01430) - 277775..278506 (+) 732 WP_349279889.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42018.51 Da        Isoelectric Point: 6.9659

>NTDB_id=1008043 ABLV49_RS01415 WP_349279887.1 274837..275973(-) (pilU) [Polaromonas hydrogenivorans strain DSM 17735]
MERDQASKFINDLLRLMVSRGGSDLFVTGEFPPAIKVDGKLTKVSSQPLNAGHTLTLARAIMNDKQAAEFERTKECNFAI
SPPGIGRFRVNAFMQQGKVGMVMRVIPAVLPTLDGLGLPPVLKDVVMAKRGLAILVGATGSGKSTTMAAMLDWRNEQSYG
HILTIEDPVEFVHMHKNCVVTQREIGLDTDSWESALKNSMRQAPDVILMGEIRDRESMEHALAFSETGHLCLATLHANSA
NQAMDRIINFFPEERRQQLLMDLSLNLKAVVSQRLVPRQDSKGRFAAVEVMLNSPLICDLIFKGDVSEIKEIMKKSRNMG
MQTFDQALFDAFEGNHILYEEALRNADSVNDLRLQIKLYSQRAKSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1008043 ABLV49_RS01415 WP_349279887.1 274837..275973(-) (pilU) [Polaromonas hydrogenivorans strain DSM 17735]
ATGGAACGCGATCAGGCGAGTAAATTCATCAATGACCTGCTGCGGCTGATGGTCAGCCGCGGCGGGAGCGACCTGTTTGT
GACCGGAGAGTTTCCGCCCGCCATCAAGGTCGATGGCAAGCTGACCAAGGTGTCGTCCCAGCCGCTCAATGCCGGCCACA
CGCTGACGCTGGCGCGCGCCATCATGAATGACAAGCAGGCCGCCGAGTTCGAGCGCACCAAGGAATGCAATTTCGCCATC
TCGCCGCCCGGCATAGGGCGCTTTCGCGTCAACGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGATGCGGGTCATTCC
GGCGGTGCTCCCCACGCTCGATGGCCTGGGCCTGCCGCCGGTGCTCAAGGACGTGGTGATGGCAAAGCGCGGCCTGGCCA
TTCTGGTGGGTGCCACCGGCTCGGGGAAATCGACCACCATGGCAGCCATGCTGGACTGGCGCAACGAACAGTCCTACGGC
CACATCCTGACGATTGAAGACCCGGTGGAGTTTGTCCACATGCACAAGAACTGCGTGGTGACCCAGCGCGAGATCGGACT
GGACACCGACAGTTGGGAATCGGCGCTGAAAAACTCGATGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAAGCATGGAGCATGCCCTGGCCTTTTCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCATGGACCGCATCATCAATTTTTTCCCGGAAGAGCGCCGCCAGCAGCTACTGATGGACCTGTCGCTCAACCT
CAAGGCCGTGGTGTCGCAGCGGCTGGTGCCCCGGCAGGACAGCAAGGGGCGCTTTGCAGCGGTCGAGGTGATGCTCAATT
CCCCCTTGATTTGCGACCTCATCTTCAAGGGCGATGTCAGCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACATGGGC
ATGCAGACCTTTGACCAGGCGCTGTTTGATGCCTTTGAAGGCAACCATATTCTTTACGAAGAGGCCTTGCGCAATGCCGA
TTCGGTCAATGACCTGCGGCTGCAGATCAAGCTTTATTCGCAACGCGCCAAGTCTACCGATCTGTCGGCAGGCACCGAGC
ACTTTGCCATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.172

92.328

0.556
  pilU Acinetobacter baylyi ADP1

55.307

94.709

0.524
  pilU Vibrio cholerae strain A1552

53.448

92.063

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Legionella pneumophila strain Lp02

41.056

90.212

0.37
  pilT Legionella pneumophila strain ERS1305867

41.056

90.212

0.37
  pilT Pseudomonas aeruginosa PAK

43.574

84.392

0.368
  pilT Acinetobacter baumannii strain A118

43.26

84.392

0.365
  pilT Acinetobacter baumannii D1279779

43.26

84.392

0.365
  pilT Acinetobacter nosocomialis M2

43.26

84.392

0.365

Multiple sequence alignment