Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABL839_RS26555 Genome accession   NZ_CP157634
Coordinates   5692345..5693388 (+) Length   347 a.a.
NCBI ID   WP_019657566.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 110B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5687345..5698388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL839_RS26525 (ABL839_26550) - 5687989..5688510 (-) 522 WP_019657560.1 alpha-ketoglutarate-dependent dioxygenase AlkB family protein -
  ABL839_RS26530 (ABL839_26555) - 5688545..5689819 (-) 1275 WP_019657561.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL839_RS26535 (ABL839_26560) - 5690055..5690378 (+) 324 WP_020723340.1 DUF4148 domain-containing protein -
  ABL839_RS26540 (ABL839_26565) - 5690504..5690962 (+) 459 WP_019657563.1 hypothetical protein -
  ABL839_RS26545 (ABL839_26570) - 5691111..5691578 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL839_RS26550 (ABL839_26575) - 5691589..5692293 (-) 705 WP_019657565.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL839_RS26555 (ABL839_26580) pilT 5692345..5693388 (+) 1044 WP_019657566.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL839_RS26560 (ABL839_26585) pilU 5693525..5694661 (+) 1137 WP_019657567.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL839_RS26565 (ABL839_26590) - 5694675..5695301 (-) 627 WP_019657568.1 TetR/AcrR family transcriptional regulator -
  ABL839_RS26570 (ABL839_26595) - 5695358..5696089 (+) 732 WP_019657569.1 nitroreductase family protein -
  ABL839_RS26575 (ABL839_26600) - 5696171..5697724 (-) 1554 WP_019657570.1 MFS transporter -
  ABL839_RS26580 (ABL839_26605) - 5697721..5698203 (-) 483 WP_019657571.1 MerR family transcriptional regulator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38208.88 Da        Isoelectric Point: 6.8356

>NTDB_id=1007898 ABL839_RS26555 WP_019657566.1 5692345..5693388(+) (pilT) [Variovorax paradoxus strain 110B]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQSRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1007898 ABL839_RS26555 WP_019657566.1 5692345..5693388(+) (pilT) [Variovorax paradoxus strain 110B]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTTTCCGCGGGCCTGCCGCCGAT
GATCCGCGTCAACGGCGACGTGCGCCGCATCAACGTCGATGCGCTCGACCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCCGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCCAAGATTTTCGGCGAACTGGCGCTCAAGCCACGCGGGCTGGTGCTGGTGACGGGTCCCACGGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCGTTCTCCAA
CGCCCTGCGCTCCGCCCTGCGCGAAGACCCCGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CGATGACCGCGGCGGAGACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAGGAGAAGGAAATGATCCGCGCCATGCTCTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGAGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTCGACCAGAAC
CTGACGGAACTGGTGCGCCGCAATACCATCTCGGCAGCGGAAGCCCGCAGCAAGGCGAAGATTCCCGAGAACTTCCCCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment