Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABL839_RS26560 Genome accession   NZ_CP157634
Coordinates   5693525..5694661 (+) Length   378 a.a.
NCBI ID   WP_019657567.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 110B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5688525..5699661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL839_RS26530 (ABL839_26555) - 5688545..5689819 (-) 1275 WP_019657561.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL839_RS26535 (ABL839_26560) - 5690055..5690378 (+) 324 WP_020723340.1 DUF4148 domain-containing protein -
  ABL839_RS26540 (ABL839_26565) - 5690504..5690962 (+) 459 WP_019657563.1 hypothetical protein -
  ABL839_RS26545 (ABL839_26570) - 5691111..5691578 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL839_RS26550 (ABL839_26575) - 5691589..5692293 (-) 705 WP_019657565.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL839_RS26555 (ABL839_26580) pilT 5692345..5693388 (+) 1044 WP_019657566.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL839_RS26560 (ABL839_26585) pilU 5693525..5694661 (+) 1137 WP_019657567.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL839_RS26565 (ABL839_26590) - 5694675..5695301 (-) 627 WP_019657568.1 TetR/AcrR family transcriptional regulator -
  ABL839_RS26570 (ABL839_26595) - 5695358..5696089 (+) 732 WP_019657569.1 nitroreductase family protein -
  ABL839_RS26575 (ABL839_26600) - 5696171..5697724 (-) 1554 WP_019657570.1 MFS transporter -
  ABL839_RS26580 (ABL839_26605) - 5697721..5698203 (-) 483 WP_019657571.1 MerR family transcriptional regulator -
  ABL839_RS26585 (ABL839_26610) - 5698293..5699201 (+) 909 WP_019657572.1 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41808.95 Da        Isoelectric Point: 6.4175

>NTDB_id=1007899 ABL839_RS26560 WP_019657567.1 5693525..5694661(+) (pilU) [Variovorax paradoxus strain 110B]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSISFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1007899 ABL839_RS26560 WP_019657567.1 5693525..5694661(+) (pilU) [Variovorax paradoxus strain 110B]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCCGACTTTCCGCCAGCCATCAAGGTCGACGGCAAGGTCACCAAGGTCTCGCAGCAGGCGCTGGGCGCGCCGCACA
CGCTGGCGCTGACCCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCGAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTCTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACTGGCGCAACGAGAACTCCTTCGGC
CACATCGTGACGGTGGAGGACCCGGTCGAGTTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGTG
ACCGCGAGACCATGGAGCACGCGGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGATCTCGCAGCGCCTGGTGCCGACCGAAGACGGCCTGGGCCGCGTGGCCGCGGTGGAGGTGCTGCTGAACA
CGCCGTTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCAGCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGTGCACGCAGCACCGACCTGGCTGCGGGCACGGAGC
ACTTCGCGATCGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.444

96.032

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

43.465

87.037

0.378
  pilT Legionella pneumophila strain ERS1305867

43.465

87.037

0.378
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment