Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABL838_RS28490 Genome accession   NZ_CP157624
Coordinates   6182266..6183309 (+) Length   347 a.a.
NCBI ID   WP_093206426.1    Uniprot ID   A0A7Y7IBT6
Organism   Variovorax sp. 160MFSha2.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6177266..6188309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL838_RS28460 (ABL838_28425) - 6177773..6178435 (-) 663 WP_418118133.1 nucleoside/nucleotide kinase family protein -
  ABL838_RS28465 (ABL838_28430) - 6178472..6179746 (-) 1275 WP_418123025.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL838_RS28470 (ABL838_28435) - 6179977..6180303 (+) 327 WP_418123026.1 DUF4148 domain-containing protein -
  ABL838_RS28475 (ABL838_28440) - 6180430..6180888 (+) 459 WP_126025784.1 hypothetical protein -
  ABL838_RS28480 (ABL838_28445) - 6181032..6181499 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL838_RS28485 (ABL838_28450) - 6181510..6182214 (-) 705 WP_418125924.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL838_RS28490 (ABL838_28455) pilT 6182266..6183309 (+) 1044 WP_093206426.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL838_RS28495 (ABL838_28460) pilU 6183338..6184474 (+) 1137 WP_126025782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL838_RS28500 (ABL838_28465) - 6184539..6185447 (+) 909 WP_418123027.1 NAD(P)-dependent oxidoreductase -
  ABL838_RS28505 (ABL838_28470) - 6185502..6186323 (-) 822 WP_418123028.1 BON domain-containing protein -
  ABL838_RS28510 (ABL838_28475) - 6186320..6186931 (-) 612 WP_126025779.1 SIS domain-containing protein -
  ABL838_RS28515 (ABL838_28480) - 6187017..6187394 (-) 378 WP_126025778.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38148.83 Da        Isoelectric Point: 6.8356

>NTDB_id=1007806 ABL838_RS28490 WP_093206426.1 6182266..6183309(+) (pilT) [Variovorax sp. 160MFSha2.1]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1007806 ABL838_RS28490 WP_093206426.1 6182266..6183309(+) (pilT) [Variovorax sp. 160MFSha2.1]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTTTCCGCGGGCCTGCCGCCCAT
GATCCGCGTCAACGGCGACGTGCGCCGCATCAACGTCGACGCGCTCGACCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGGGTGAACGCCTTCAACCAGGCACGCGGCGCGGCCGCGGTGTTCCGGACCATTCCGTCCAAGATCCTCACGCTCGA
GCAGCTGAACGCGCCGAAGATTTTCGGTGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGGGACCGATGACACTGTCGTTCTCCAA
CGCCCTGCGCTCCGCGCTGCGCGAAGACCCCGACGCCATCCTGGTGGGCGAGCTGCGCGACCTCGAAACCATCCGCCTGG
CCATGACCGCCGCGGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCCTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAGGAAATGATCCGCGCCATGCTCTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACAAAGGACGGCCAGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACCCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTGGACCAGAAC
CTGACGGAACTGGTGCGCCGCAATACCATCTCGGCAGCGGAAGCCCGCGGGAAAGCCAAGATCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y7IBT6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

74.336

97.695

0.726
  pilT Legionella pneumophila strain ERS1305867

74.336

97.695

0.726
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

44.179

96.542

0.427
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401

Multiple sequence alignment