Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABL838_RS28495 Genome accession   NZ_CP157624
Coordinates   6183338..6184474 (+) Length   378 a.a.
NCBI ID   WP_126025782.1    Uniprot ID   A0A433MVA6
Organism   Variovorax sp. 160MFSha2.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6178338..6189474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL838_RS28465 (ABL838_28430) - 6178472..6179746 (-) 1275 WP_418123025.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL838_RS28470 (ABL838_28435) - 6179977..6180303 (+) 327 WP_418123026.1 DUF4148 domain-containing protein -
  ABL838_RS28475 (ABL838_28440) - 6180430..6180888 (+) 459 WP_126025784.1 hypothetical protein -
  ABL838_RS28480 (ABL838_28445) - 6181032..6181499 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL838_RS28485 (ABL838_28450) - 6181510..6182214 (-) 705 WP_418125924.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL838_RS28490 (ABL838_28455) pilT 6182266..6183309 (+) 1044 WP_093206426.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL838_RS28495 (ABL838_28460) pilU 6183338..6184474 (+) 1137 WP_126025782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL838_RS28500 (ABL838_28465) - 6184539..6185447 (+) 909 WP_418123027.1 NAD(P)-dependent oxidoreductase -
  ABL838_RS28505 (ABL838_28470) - 6185502..6186323 (-) 822 WP_418123028.1 BON domain-containing protein -
  ABL838_RS28510 (ABL838_28475) - 6186320..6186931 (-) 612 WP_126025779.1 SIS domain-containing protein -
  ABL838_RS28515 (ABL838_28480) - 6187017..6187394 (-) 378 WP_126025778.1 YraN family protein -
  ABL838_RS28520 (ABL838_28485) rsmI 6187464..6188414 (+) 951 WP_126025777.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ABL838_RS28525 (ABL838_28490) - 6188411..6189139 (-) 729 WP_126025776.1 glutathione S-transferase family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41810.92 Da        Isoelectric Point: 6.4175

>NTDB_id=1007807 ABL838_RS28495 WP_126025782.1 6183338..6184474(+) (pilU) [Variovorax sp. 160MFSha2.1]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSISFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1007807 ABL838_RS28495 WP_126025782.1 6183338..6184474(+) (pilU) [Variovorax sp. 160MFSha2.1]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTTTCGCAGCAGGCGCTGGGCGCGCCGCACA
CGCTGGCGCTGACCCGCTCGATCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCGAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTGACGGTGGAAGACCCGGTCGAATTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTCATTCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCCGTGGCTTTCGCCGAAACGGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAACGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTTTCGCAACGGCTGGTGCCGACGGAAGACGGCCTGGGCCGCGTGGCCGCGGTGGAAGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAAATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTTCGAGAGCCACTCGATCAGCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCGGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A433MVA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376

Multiple sequence alignment