Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABL843_RS28315 Genome accession   NZ_CP157620
Coordinates   6235606..6236649 (+) Length   347 a.a.
NCBI ID   WP_126025783.1    Uniprot ID   A0A3S0XJW7
Organism   Variovorax sp. 350MFTsu5.1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6230606..6241649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL843_RS28285 (ABL843_28280) - 6231100..6231762 (-) 663 WP_418118133.1 nucleoside/nucleotide kinase family protein -
  ABL843_RS28290 (ABL843_28285) - 6231799..6233073 (-) 1275 WP_418118134.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL843_RS28295 (ABL843_28290) - 6233308..6233634 (+) 327 WP_418118136.1 DUF4148 domain-containing protein -
  ABL843_RS28300 (ABL843_28295) - 6233761..6234228 (+) 468 WP_418118138.1 hypothetical protein -
  ABL843_RS28305 (ABL843_28300) - 6234372..6234839 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL843_RS28310 (ABL843_28305) - 6234850..6235554 (-) 705 WP_418118139.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL843_RS28315 (ABL843_28310) pilT 6235606..6236649 (+) 1044 WP_126025783.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL843_RS28320 (ABL843_28315) pilU 6236786..6237922 (+) 1137 WP_126025782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL843_RS28325 (ABL843_28320) - 6237987..6238895 (+) 909 WP_418118140.1 NAD(P)-dependent oxidoreductase -
  ABL843_RS28330 (ABL843_28325) - 6238950..6239768 (-) 819 WP_126025780.1 BON domain-containing protein -
  ABL843_RS28335 (ABL843_28330) - 6239765..6240376 (-) 612 WP_126025779.1 SIS domain-containing protein -
  ABL843_RS28340 (ABL843_28335) - 6240462..6240839 (-) 378 WP_418118142.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38178.86 Da        Isoelectric Point: 6.8356

>NTDB_id=1007768 ABL843_RS28315 WP_126025783.1 6235606..6236649(+) (pilT) [Variovorax sp. 350MFTsu5.1]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQSRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1007768 ABL843_RS28315 WP_126025783.1 6235606..6236649(+) (pilT) [Variovorax sp. 350MFTsu5.1]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTTTCCGCGGGCCTGCCGCCCAT
GATCCGCGTCAACGGCGACGTGCGCCGCATCAACGTCGACGCGCTCGACCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCCGCGGTGTTCCGGACCATTCCGTCCAAGATCCTCACGCTCGA
GCAGCTGAACGCGCCGAAGATTTTCGGTGAACTGGCGCTCAAGCCGCGCGGGCTGGTGCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAGGTGGGACCGATGACACTGTCGTTCTCCAA
CGCCCTGCGCTCCGCGCTGCGCGAAGACCCCGACGCCATCCTGGTGGGCGAGCTGCGCGACCTCGAAACCATCCGCCTGG
CCATGACCGCCGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCCTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAGGAAATGATCCGCGCCATGCTCTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACAAAGGACGGCCAGAGCCGCGTGGCGGCGCACGAAATCATGCTGGGCACCCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCGGGCATGCAGACGCTGGACCAGAAC
CTGACGGAACTGGTGCGCCGCAATACCATCTCGGCAGCGGAAGCCCGCGGGAAAGCCAAGATCCCCGAGAACTTCCCCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0XJW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.328

98.271

0.671
  pilT Vibrio cholerae strain A1552

68.328

98.271

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment