Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABL843_RS28320 Genome accession   NZ_CP157620
Coordinates   6236786..6237922 (+) Length   378 a.a.
NCBI ID   WP_126025782.1    Uniprot ID   A0A433MVA6
Organism   Variovorax sp. 350MFTsu5.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6231786..6242922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL843_RS28290 (ABL843_28285) - 6231799..6233073 (-) 1275 WP_418118134.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL843_RS28295 (ABL843_28290) - 6233308..6233634 (+) 327 WP_418118136.1 DUF4148 domain-containing protein -
  ABL843_RS28300 (ABL843_28295) - 6233761..6234228 (+) 468 WP_418118138.1 hypothetical protein -
  ABL843_RS28305 (ABL843_28300) - 6234372..6234839 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL843_RS28310 (ABL843_28305) - 6234850..6235554 (-) 705 WP_418118139.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL843_RS28315 (ABL843_28310) pilT 6235606..6236649 (+) 1044 WP_126025783.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL843_RS28320 (ABL843_28315) pilU 6236786..6237922 (+) 1137 WP_126025782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL843_RS28325 (ABL843_28320) - 6237987..6238895 (+) 909 WP_418118140.1 NAD(P)-dependent oxidoreductase -
  ABL843_RS28330 (ABL843_28325) - 6238950..6239768 (-) 819 WP_126025780.1 BON domain-containing protein -
  ABL843_RS28335 (ABL843_28330) - 6239765..6240376 (-) 612 WP_126025779.1 SIS domain-containing protein -
  ABL843_RS28340 (ABL843_28335) - 6240462..6240839 (-) 378 WP_418118142.1 YraN family protein -
  ABL843_RS28345 (ABL843_28340) rsmI 6240912..6241862 (+) 951 WP_418118144.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ABL843_RS28350 (ABL843_28345) - 6241859..6242587 (-) 729 WP_184642083.1 glutathione S-transferase family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41810.92 Da        Isoelectric Point: 6.4175

>NTDB_id=1007769 ABL843_RS28320 WP_126025782.1 6236786..6237922(+) (pilU) [Variovorax sp. 350MFTsu5.1]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSISFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1007769 ABL843_RS28320 WP_126025782.1 6236786..6237922(+) (pilU) [Variovorax sp. 350MFTsu5.1]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTTTCGCAGCAGGCGCTGGGCGCGCCGCACA
CGCTGGCGCTGACCCGCTCGATCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTGGCGCAACGAGAACTCCTACGGC
CACATCGTGACGGTGGAAGACCCGGTCGAATTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTCATTCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCCGTGGCTTTCGCCGAAACGGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAACGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTTTCGCAACGGCTGGTGCCAACGGAAGACGGCCTGGGCCGCGTGGCCGCGGTGGAAGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAAATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCAGCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTGGCGGCGGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A433MVA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

45.401

89.153

0.405
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376

Multiple sequence alignment