Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABL847_RS22320 Genome accession   NZ_CP157616
Coordinates   4798654..4799697 (-) Length   347 a.a.
NCBI ID   WP_076998014.1    Uniprot ID   -
Organism   Variovorax sp. KK3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4793654..4804697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL847_RS22300 (ABL847_22260) - 4794072..4795829 (+) 1758 WP_179106834.1 hybrid sensor histidine kinase/response regulator -
  ABL847_RS22305 (ABL847_22265) - 4795789..4796637 (-) 849 WP_076998011.1 helix-turn-helix domain-containing protein -
  ABL847_RS22310 (ABL847_22270) - 4796818..4797348 (+) 531 WP_076998012.1 JAB domain-containing protein -
  ABL847_RS22315 (ABL847_22275) pilU 4797376..4798512 (-) 1137 WP_076998013.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL847_RS22320 (ABL847_22280) pilT 4798654..4799697 (-) 1044 WP_076998014.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL847_RS22325 (ABL847_22285) - 4799736..4800422 (+) 687 WP_076998015.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL847_RS22330 (ABL847_22290) - 4800561..4801832 (+) 1272 WP_076998016.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL847_RS22335 (ABL847_22295) - 4801958..4802629 (+) 672 WP_076998017.1 DUF2242 domain-containing protein -
  ABL847_RS22340 (ABL847_22300) - 4802631..4802858 (-) 228 WP_076998018.1 hypothetical protein -
  ABL847_RS22345 (ABL847_22305) - 4803048..4803425 (+) 378 WP_076998019.1 VOC family protein -
  ABL847_RS22350 (ABL847_22310) rocF 4803474..4804388 (-) 915 WP_076998020.1 arginase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38133.94 Da        Isoelectric Point: 6.8845

>NTDB_id=1007729 ABL847_RS22320 WP_076998014.1 4798654..4799697(-) (pilT) [Variovorax sp. KK3]
MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINVDALDHKAVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINFLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFANALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKGGDGRVAAHEIMLGTPAIRNLIRENKVAQMYSSIQTGVNAGMQTLDQC
LTDLVRRNVISAAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1007729 ABL847_RS22320 WP_076998014.1 4798654..4799697(-) (pilT) [Variovorax sp. KK3]
GTGGACATCACCCAACTGCTGGCTTTCAGCGTCAAGAACAAGGCGTCGGACTTGCACCTGTCGTCAGGGCTGCCCCCGAT
GATCCGGGTTCACGGCGACGTGCGGCGCATCAACGTCGACGCGCTCGACCACAAGGCGGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCATTACGAAGAGTTCCTGGAGGTCGATTTCTCTTTCGAAATCGACGGCCTCGCGCGC
TTCCGCGTGAATGCCTTCAACCAGGCCCGGGGCGCGGCAGCCGTGTTCCGGACCATCCCGTCGAAGATCCTCACCCTCGA
GCAGCTCAACGCGCCCAAGATTTTCGGCGAACTCGCACTCAAGCCGCGCGGGCTGGTGCTGGTTACCGGGCCCACCGGCT
CCGGCAAGTCGACCACGCTGGCCGCGATGATCAACTTCCTCAACGAAACCGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGGCCGATGACGCTGTCCTTCGCCAA
CGCGCTGCGCTCCGCGCTGCGTGAAGATCCCGACGCCATCCTGGTCGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CCATGACCGCGGCCGAAACCGGCCACCTCGTGTTCGGCACCCTGCACACGTCTTCCGCGGCCAAAACCATCGACCGGATC
ATCGACGTCTTCCCCGGCGAAGAAAAAGAAATGATCCGCGCCATGCTGTCCGAGTCGCTGCAAGCCGTCATCTCGCAGAC
GCTGTGCAAGACCAAGGGCGGCGACGGGCGCGTCGCGGCGCACGAGATCATGCTGGGCACCCCCGCCATCCGCAACCTGA
TCCGCGAGAACAAGGTCGCGCAGATGTACTCGTCGATCCAGACCGGCGTCAACGCAGGCATGCAGACGCTCGACCAATGC
CTGACGGACCTCGTGCGGCGCAACGTCATCTCGGCGGCCGAGGCACGAAGCAAAGCGAAGATTCCCGAAAACTTTCCCGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

68.116

99.424

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.328

98.271

0.671
  pilT Vibrio cholerae strain A1552

68.328

98.271

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

40.173

99.712

0.401
  pilU Pseudomonas stutzeri DSM 10701

40

96.542

0.386

Multiple sequence alignment