Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABL847_RS22315 Genome accession   NZ_CP157616
Coordinates   4797376..4798512 (-) Length   378 a.a.
NCBI ID   WP_076998013.1    Uniprot ID   -
Organism   Variovorax sp. KK3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4792376..4803512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL847_RS22290 (ABL847_22250) - 4792795..4793559 (+) 765 WP_076998008.1 SDR family oxidoreductase -
  ABL847_RS22295 (ABL847_22255) - 4793579..4794082 (+) 504 WP_076998009.1 molybdopterin-dependent oxidoreductase -
  ABL847_RS22300 (ABL847_22260) - 4794072..4795829 (+) 1758 WP_179106834.1 hybrid sensor histidine kinase/response regulator -
  ABL847_RS22305 (ABL847_22265) - 4795789..4796637 (-) 849 WP_076998011.1 helix-turn-helix domain-containing protein -
  ABL847_RS22310 (ABL847_22270) - 4796818..4797348 (+) 531 WP_076998012.1 JAB domain-containing protein -
  ABL847_RS22315 (ABL847_22275) pilU 4797376..4798512 (-) 1137 WP_076998013.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL847_RS22320 (ABL847_22280) pilT 4798654..4799697 (-) 1044 WP_076998014.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL847_RS22325 (ABL847_22285) - 4799736..4800422 (+) 687 WP_076998015.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL847_RS22330 (ABL847_22290) - 4800561..4801832 (+) 1272 WP_076998016.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL847_RS22335 (ABL847_22295) - 4801958..4802629 (+) 672 WP_076998017.1 DUF2242 domain-containing protein -
  ABL847_RS22340 (ABL847_22300) - 4802631..4802858 (-) 228 WP_076998018.1 hypothetical protein -
  ABL847_RS22345 (ABL847_22305) - 4803048..4803425 (+) 378 WP_076998019.1 VOC family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42024.22 Da        Isoelectric Point: 6.3467

>NTDB_id=1007728 ABL847_RS22315 WP_076998013.1 4797376..4798512(-) (pilU) [Variovorax sp. KK3]
MERDQASQFINELLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGVGRFRVNAFVQQGKVGMVLRTIPATLPTIDGLNMPQILKDVTMTKRGLCILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFIHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLIPTEDGHGRVAAVEILLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFETHMISFEDALRNADSANDLRLQIKLNSQRARTTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1007728 ABL847_RS22315 WP_076998013.1 4797376..4798512(-) (pilU) [Variovorax sp. KK3]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGAACTGCTCAAGCTGATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGAGTTTCCGCCGGCGATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCCCTTGGCGCGCAGCACA
CGCTGGCGCTGACGCGCTCGATCATGAACGACCGTCAGACGGCCGAGTTCGAACGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCGTCGGCCGCTTCCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTGGGCATGGTGCTGCGGACCATCCC
GGCCACGCTGCCCACCATCGACGGCCTGAACATGCCGCAGATCCTCAAGGACGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAAAACTCCTACGGC
CACATCGTCACCGTCGAAGACCCGGTGGAATTCATCCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTCGGCAT
CGACACCGATAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCACCCGACGTCATCCTCATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACACGCGGTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCATGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAATTGCTGATGGACCTGTCGCTCAACCT
GCGTTCGCTGGTCTCGCAGCGCCTCATCCCCACCGAAGACGGCCATGGCCGCGTGGCCGCGGTCGAGATCCTGCTCAACA
CCCCGCTGATCAGCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGACGCACATGATTTCTTTCGAAGACGCGCTGCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCACCACGGACCTGGCGGCGGGCACGGAGC
ATTTCGCGATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.143

92.593

0.585
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

44.377

87.037

0.386
  pilT Legionella pneumophila strain ERS1305867

44.377

87.037

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.849

83.862

0.368
  pilT Vibrio cholerae strain A1552

43.849

83.862

0.368

Multiple sequence alignment