Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABLB96_RS07020 Genome accession   NZ_CP157428
Coordinates   1445656..1446693 (-) Length   345 a.a.
NCBI ID   WP_107972265.1    Uniprot ID   -
Organism   Acinetobacter sp. XH1741     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1440656..1451693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLB96_RS06990 (ABLB96_06990) - 1441439..1441894 (+) 456 WP_044099926.1 bacteriohemerythrin -
  ABLB96_RS06995 (ABLB96_06995) - 1442047..1443114 (+) 1068 WP_348897795.1 hypothetical protein -
  ABLB96_RS07000 (ABLB96_07000) - 1443114..1443437 (+) 324 WP_348897796.1 RnfH family protein -
  ABLB96_RS07005 (ABLB96_07005) - 1443493..1443891 (-) 399 WP_348897797.1 outer membrane protein assembly factor BamE -
  ABLB96_RS07010 (ABLB96_07010) fur 1444003..1444440 (+) 438 WP_309456688.1 ferric iron uptake transcriptional regulator -
  ABLB96_RS07015 (ABLB96_07015) pilU 1444510..1445628 (-) 1119 WP_348897798.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABLB96_RS07020 (ABLB96_07020) pilT 1445656..1446693 (-) 1038 WP_107972265.1 type IV pilus twitching motility protein PilT Machinery gene
  ABLB96_RS07025 (ABLB96_07025) - 1446820..1447512 (+) 693 WP_348897799.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABLB96_RS07030 (ABLB96_07030) - 1447620..1448462 (+) 843 WP_348897800.1 undecaprenyl-diphosphate phosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38404.00 Da        Isoelectric Point: 6.7271

>NTDB_id=1006772 ABLB96_RS07020 WP_107972265.1 1445656..1446693(-) (pilT) [Acinetobacter sp. XH1741]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1006772 ABLB96_RS07020 WP_107972265.1 1445656..1446693(-) (pilT) [Acinetobacter sp. XH1741]
ATGGATATTACAGAGCTACTTGCCTTCTCTGTGAAAAATGGCGCTTCCGATTTACACTTGTCTGCTGGCATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGCCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGCCTTGTTTATGACA
TCATGAACGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACCGACTTTTCTTTTGAAGTACCTAATGTTGCCCGT
TTTCGTGTGAACGCATTTAACCAAAACCGTGGTGCTGGCGCGGTATTTCGTACCATTCCATCTAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGATATCTGTGATTATCCTCGTGGGATTGTACTGGTGACAGGTCCAACCGGTT
CGGGTAAATCAACCACCCTTGCAGCGATGATGGACTACATTAATGAAAATCGCTATGACCATATTTTAACGGTTGAAGAC
CCCATCGAATTTGTACATCAGTCAAAGAAATGCCTGATTAACCAGCGTGAAGTACATCGTGATACACATGGCTTTAATGA
AGCACTTCGCTCAGCACTACGTGAAGACCCAGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGTTTAG
CATTAACTGCTGCCGAAACGGGTCACTTGGTGTTCGGTACACTTCATACGACTTCTGCCGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCCGTTATTTCACAAAC
CTTACTTAAAAAGAACGGTGGTGGTCGTGTAGCAGCACATGAAATCATGATTGGTATTCCTGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTATTCAGCCATTCAGACGGGTGCTAACCATGGTATGACGACCCTCGATCAAAGCTTA
AAAAACTTAGTGTCTCGTGGTGTAATTAGCCCACAAACGGCGCGTACTGCGGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.42

100

0.994
  pilT Acinetobacter baumannii D1279779

99.13

100

0.991
  pilT Acinetobacter baumannii strain A118

99.13

100

0.991
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment