Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABHF33_RS12405 Genome accession   NZ_CP157355
Coordinates   2624874..2625917 (+) Length   347 a.a.
NCBI ID   WP_157669675.1    Uniprot ID   A0AAU7F871
Organism   Chitinibacter sp. FCG-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2619874..2630917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF33_RS12375 (ABHF33_12375) dsbD 2620169..2621734 (-) 1566 WP_348944239.1 protein-disulfide reductase DsbD -
  ABHF33_RS12380 (ABHF33_12380) cutA 2621731..2622081 (-) 351 WP_348944240.1 divalent-cation tolerance protein CutA -
  ABHF33_RS12385 (ABHF33_12385) - 2622080..2622511 (+) 432 WP_348944241.1 FxsA family protein -
  ABHF33_RS12390 (ABHF33_12390) - 2622647..2623234 (-) 588 WP_348944242.1 YggT family protein -
  ABHF33_RS12395 (ABHF33_12395) proC 2623259..2624077 (-) 819 WP_348944243.1 pyrroline-5-carboxylate reductase -
  ABHF33_RS12400 (ABHF33_12400) - 2624086..2624781 (-) 696 WP_348944244.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABHF33_RS12405 (ABHF33_12405) pilT 2624874..2625917 (+) 1044 WP_157669675.1 type IV pilus twitching motility protein PilT Machinery gene
  ABHF33_RS12410 (ABHF33_12410) pilU 2625937..2627073 (+) 1137 WP_348944245.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHF33_RS12415 (ABHF33_12415) - 2627077..2629326 (+) 2250 WP_348944246.1 hypothetical protein -
  ABHF33_RS12420 (ABHF33_12420) - 2629466..2629867 (+) 402 WP_348944247.1 SMI1/KNR4 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38278.03 Da        Isoelectric Point: 6.8841

>NTDB_id=1006248 ABHF33_RS12405 WP_157669675.1 2624874..2625917(+) (pilT) [Chitinibacter sp. FCG-7]
MEISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMDHKDVHDMVYDIMNDGQRKIYEDTLECDFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLTLEELNAPKVFQDIAMNPRGLVLVTGPTGSGKSTTLAAMINFINDNEYGHILTVED
PIEFVHQSKKCLVNQREVGPHTLSFANALRSALREDPDIVLVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMIGIPAIRNLIRENKVAQMYSSIQTGSAHGMQTLDQC
LQNLVQRNIVSVAEARNKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1006248 ABHF33_RS12405 WP_157669675.1 2624874..2625917(+) (pilT) [Chitinibacter sp. FCG-7]
ATGGAAATCTCGGAACTCTTAGCGTTTTCGGTCAAGAATAAGGCATCGGACTTGCACCTGTCGGCAGGGTTGCCACCGAT
GATCCGGGTTCATGGCGATGTGCGCCGCATCAATCTGCCTGCAATGGATCATAAAGACGTACACGATATGGTGTACGACA
TCATGAACGATGGTCAGCGCAAGATTTATGAAGACACGCTGGAATGCGATTTTTCGTTTGAAATTCCCAATTTGGCGCGT
TTTCGGGTCAATGCCTTTGTGCAAAACCGGGGTGCTGGCGCAGTATTTCGGACCATTCCGTCCAAGGTGTTGACGCTGGA
AGAGCTCAATGCGCCTAAAGTGTTCCAGGATATTGCCATGAATCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACTGGCT
CGGGTAAATCCACCACGTTGGCGGCGATGATCAACTTCATCAATGACAATGAATATGGCCATATTCTGACGGTGGAAGAC
CCGATCGAGTTTGTCCACCAATCCAAAAAATGTCTGGTCAACCAGCGTGAAGTCGGCCCGCACACCCTGTCATTTGCCAA
CGCCTTGCGCTCTGCCTTGCGTGAAGACCCGGATATTGTACTGGTCGGCGAGATGCGCGATCTGGAAACAATCCGTCTGG
CTTTAACTGCGGCGGAAACCGGGCATTTGGTGTTTGGTACTTTGCATACCAGCTCGGCAGCGAAAACGATTGACCGTATC
GTCGACGTATTTCCTGCTGCTGAAAAAGAAATGGTTCGCTCGATGTTGTCCGAATCATTGCGCGCTGTTATTTCGCAAAC
GCTGCTCAAGACCAAGGATGGTACAGGCCGCGTGGCCGCGCATGAAATCATGATCGGGATTCCGGCGATCCGTAACCTGA
TCCGTGAAAATAAAGTAGCGCAAATGTATTCGTCGATTCAAACCGGCTCTGCACACGGCATGCAAACTTTGGATCAATGT
CTGCAGAATCTGGTACAGCGCAATATTGTGTCGGTTGCAGAGGCGCGCAATAAAGCGGCCATTCCGGATAACTTCAAAGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Acinetobacter baylyi ADP1

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria meningitidis 8013

73.156

97.695

0.715
  pilT Neisseria gonorrhoeae MS11

72.861

97.695

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.639

97.406

0.669
  pilT Vibrio cholerae strain A1552

68.639

97.406

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

38.905

100

0.389

Multiple sequence alignment