Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABHF33_RS12410 Genome accession   NZ_CP157355
Coordinates   2625937..2627073 (+) Length   378 a.a.
NCBI ID   WP_348944245.1    Uniprot ID   A0AAU7F6Z9
Organism   Chitinibacter sp. FCG-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2620937..2632073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF33_RS12380 (ABHF33_12380) cutA 2621731..2622081 (-) 351 WP_348944240.1 divalent-cation tolerance protein CutA -
  ABHF33_RS12385 (ABHF33_12385) - 2622080..2622511 (+) 432 WP_348944241.1 FxsA family protein -
  ABHF33_RS12390 (ABHF33_12390) - 2622647..2623234 (-) 588 WP_348944242.1 YggT family protein -
  ABHF33_RS12395 (ABHF33_12395) proC 2623259..2624077 (-) 819 WP_348944243.1 pyrroline-5-carboxylate reductase -
  ABHF33_RS12400 (ABHF33_12400) - 2624086..2624781 (-) 696 WP_348944244.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABHF33_RS12405 (ABHF33_12405) pilT 2624874..2625917 (+) 1044 WP_157669675.1 type IV pilus twitching motility protein PilT Machinery gene
  ABHF33_RS12410 (ABHF33_12410) pilU 2625937..2627073 (+) 1137 WP_348944245.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHF33_RS12415 (ABHF33_12415) - 2627077..2629326 (+) 2250 WP_348944246.1 hypothetical protein -
  ABHF33_RS12420 (ABHF33_12420) - 2629466..2629867 (+) 402 WP_348944247.1 SMI1/KNR4 family protein -
  ABHF33_RS12425 (ABHF33_12425) - 2630780..2631277 (-) 498 WP_348944248.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42556.81 Da        Isoelectric Point: 6.2386

>NTDB_id=1006249 ABHF33_RS12410 WP_348944245.1 2625937..2627073(+) (pilU) [Chitinibacter sp. FCG-7]
MEKEQAAKFMHDLLRHMRSKNASDLFITVDFPPAMKIDGRVTPVSNQMLTAQHTKELARAIMNDRQSEDFEANKECNFAI
SPGTMGRFRVNAFMQQGRVGMVLRTINSEIPKLKELNLPPVLEDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENAYD
HIITIEDPIEYVHEHRNSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLKAFVSQRLVPHASGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLFEAGWITYEDALRNADSVNDLRLQIKLNGSDAKQRDVLAGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1006249 ABHF33_RS12410 WP_348944245.1 2625937..2627073(+) (pilU) [Chitinibacter sp. FCG-7]
ATGGAAAAAGAACAAGCAGCCAAGTTTATGCATGATTTGCTGCGACATATGCGCAGCAAAAATGCCTCGGATTTATTTAT
TACGGTGGATTTTCCGCCTGCAATGAAAATTGATGGCCGTGTAACACCGGTGTCGAATCAGATGTTGACTGCGCAGCACA
CCAAAGAGCTGGCGCGGGCGATTATGAACGACCGACAGTCGGAAGACTTTGAAGCCAATAAGGAATGCAATTTCGCCATC
AGCCCGGGCACGATGGGGCGCTTCCGGGTGAATGCCTTTATGCAGCAAGGCCGGGTTGGCATGGTGCTGCGGACGATCAA
CTCCGAAATTCCCAAACTCAAAGAACTGAATCTGCCGCCGGTACTGGAAGACATTGCGATGACCAAACGTGGTCTGGTAA
TTTTTGTCGGCGGTACAGGCTCGGGTAAATCGACTTCGCTGGCGGCCATGATCGGCCATCGAAATGAAAATGCATACGAT
CATATTATTACGATTGAAGACCCGATCGAGTATGTGCACGAGCACAGAAACTCGATCATTACCCAGCGCGAAGTCGGTGT
GGACACCGATTCATGGATGGCCGCGCTGAAAAATACGCTGCGGCAAGCCCCGGACGTCATTCTGATCGGTGAGATTCGTG
ATCGCGAAACAATGGATTACGCGATTGCTTTCGCCGAAACCGGCCACTTGTGCATGGCAACGCTGCACGCCAACTCGTCC
AACCAGGCGCTGGACCGGATTATCAACTTTTTCCCCGAAGAGCGTCGCCAGCAATTGCTGATGGATTTGTCACTCAATTT
GAAAGCGTTTGTTTCGCAACGCTTGGTGCCGCATGCATCGGGCAAAGGCCGAGTTGCTGCAGTTGAAGTTATGCTCAATT
CACCGCTGATTTCCGAGCTGATCTTCAAGGGTGAAGTTCATGAAATCAAAGAAATCATGAAAAAATCCCGCGAGCTGGGG
ATGCAGACATTTGATCAGTCTCTGTTTGATTTGTTTGAAGCTGGCTGGATCACTTATGAAGATGCATTACGCAATGCCGA
CTCGGTGAATGATTTGCGTCTGCAGATCAAGCTCAATGGTTCGGATGCGAAACAGCGTGATGTGTTGGCAGGGCTTGACC
ATTTGGATATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.584

91.534

0.582
  pilU Acinetobacter baylyi ADP1

59.312

92.328

0.548
  pilU Vibrio cholerae strain A1552

52.975

93.386

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.269

88.624

0.41
  pilT Acinetobacter baylyi ADP1

42.433

89.153

0.378
  pilT Acinetobacter baumannii strain A118

41.994

87.566

0.368
  pilT Acinetobacter nosocomialis M2

41.994

87.566

0.368
  pilT Acinetobacter baumannii D1279779

41.994

87.566

0.368
  pilT Pseudomonas aeruginosa PAK

41.194

88.624

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.896

88.624

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.515

87.302

0.362
  pilT Vibrio cholerae strain A1552

41.515

87.302

0.362

Multiple sequence alignment