Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUD59_RS09420 Genome accession   NZ_AP027292
Coordinates   2040127..2041161 (+) Length   344 a.a.
NCBI ID   WP_286236662.1    Uniprot ID   -
Organism   Neptuniibacter halophilus strain LMG 25378     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2035127..2046161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD59_RS09385 - 2035156..2035605 (-) 450 WP_286236653.1 DUF4426 domain-containing protein -
  QUD59_RS09390 metW 2035618..2036208 (-) 591 WP_286236654.1 methionine biosynthesis protein MetW -
  QUD59_RS09395 - 2036208..2037377 (-) 1170 WP_286236655.1 homoserine O-acetyltransferase -
  QUD59_RS09400 - 2037512..2037802 (-) 291 WP_286236656.1 DUF167 domain-containing protein -
  QUD59_RS09405 - 2037804..2038349 (-) 546 WP_286236657.1 YggT family protein -
  QUD59_RS09410 proC 2038485..2039312 (-) 828 WP_286236658.1 pyrroline-5-carboxylate reductase -
  QUD59_RS09415 - 2039342..2040037 (-) 696 WP_286236660.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUD59_RS09420 pilT 2040127..2041161 (+) 1035 WP_286236662.1 type IV pilus twitching motility protein PilT Machinery gene
  QUD59_RS09425 pilU 2041182..2042348 (+) 1167 WP_286236663.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUD59_RS09430 - 2042422..2043072 (-) 651 WP_286236664.1 FecR family protein -
  QUD59_RS09440 smpB 2043581..2044054 (-) 474 WP_286236665.1 SsrA-binding protein SmpB -
  QUD59_RS09445 - 2044323..2045687 (+) 1365 WP_286236668.1 sodium-dependent transporter -
  QUD59_RS09450 - 2045704..2046135 (+) 432 WP_286236670.1 type II toxin-antitoxin system RatA family toxin -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38037.74 Da        Isoelectric Point: 6.6676

>NTDB_id=100620 QUD59_RS09420 WP_286236662.1 2040127..2041161(+) (pilT) [Neptuniibacter halophilus strain LMG 25378]
MDITELLSFTVQQGASDLHITAGMPPIIRVDGDVRRIKLPSLDHKQVHSLIYDIMNDKQRKEYEEVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLGMGKVFQDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFANALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKAMVRSMLSESLMGVISQTLLKKPNGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGASFGMKTLDQSL
MELLQKGMISKDTARERAANPAQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=100620 QUD59_RS09420 WP_286236662.1 2040127..2041161(+) (pilT) [Neptuniibacter halophilus strain LMG 25378]
ATGGATATTACCGAACTTTTATCATTCACCGTACAGCAGGGCGCATCCGACCTGCATATTACCGCAGGAATGCCGCCTAT
CATCCGTGTTGACGGGGATGTTCGCCGTATCAAACTGCCTTCACTGGATCACAAACAGGTCCACTCCCTGATTTATGACA
TCATGAACGATAAACAGCGTAAAGAGTACGAGGAAGTGCTCGAGACGGATTTCTCGTTCGAGGTGCCGGGTCTGGCCCGT
TTCCGTGTTAACGCCTTTAACCAGAACCGTGGTGCCGGCGCCGTATTCAGGACCATCCCCAGCAAGGTATTAACGCTGGA
TGATCTGGGTATGGGCAAAGTGTTTCAGGATCTGTCGGAAAAACCCCGTGGACTGGTACTGGTGACCGGGCCAACCGGTT
CCGGTAAGAGTACCACGCTGGCGGCGATGATCGATTACATCAATGACACTCGTGCGGATCATATCCTGACCATTGAAGAC
CCGATCGAATTCGTGCATGAAAGTAAGAAATGTCTGGTGAACCAGCGTGAAGTGCACCGTGATACCCACAGCTTTGCCAA
CGCCCTGCGTTCAGCCCTGCGTGAAGACCCGGATATTATTCTGGTGGGTGAGATGCGAGACCTCGAAACCATCCGTTTGG
CGCTGACAGCGGCGGAAACCGGTCACCTGGTATTTGGTACCCTGCATACCACCTCAGCGGCAAAAACCATTGACCGTGTG
ATCGACGTATTCCCGGCAGCAGAGAAAGCGATGGTTCGTTCTATGTTGTCTGAATCATTGATGGGTGTTATCTCTCAGAC
TCTGCTGAAAAAACCTAATGGCGGTCGTGTGGCAGCACATGAGATCATGATCGGTACTCCGGCGATTCGAAACCTGATCC
GGGAAGATAAAGTGGCACAGATGTATTCAGCCATTCAGACCGGTGCCTCATTCGGGATGAAAACGCTGGATCAGTCACTG
ATGGAGCTGCTACAGAAAGGCATGATTTCCAAGGATACAGCGCGTGAGCGGGCGGCGAACCCGGCTCAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.558

100

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.267

100

0.823
  pilT Acinetobacter nosocomialis M2

80.814

100

0.808
  pilT Acinetobacter baumannii D1279779

80.814

100

0.808
  pilT Acinetobacter baumannii strain A118

80.814

100

0.808
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

73.547

100

0.735
  pilT Legionella pneumophila strain ERS1305867

73.547

100

0.735
  pilT Neisseria meningitidis 8013

66.569

99.128

0.66
  pilT Neisseria gonorrhoeae MS11

66.276

99.128

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.389

95.64

0.654
  pilT Vibrio cholerae strain A1552

68.389

95.64

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.233

99.709

0.401

Multiple sequence alignment