Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUD59_RS09425 Genome accession   NZ_AP027292
Coordinates   2041182..2042348 (+) Length   388 a.a.
NCBI ID   WP_286236663.1    Uniprot ID   -
Organism   Neptuniibacter halophilus strain LMG 25378     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2036182..2047348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD59_RS09395 - 2036208..2037377 (-) 1170 WP_286236655.1 homoserine O-acetyltransferase -
  QUD59_RS09400 - 2037512..2037802 (-) 291 WP_286236656.1 DUF167 domain-containing protein -
  QUD59_RS09405 - 2037804..2038349 (-) 546 WP_286236657.1 YggT family protein -
  QUD59_RS09410 proC 2038485..2039312 (-) 828 WP_286236658.1 pyrroline-5-carboxylate reductase -
  QUD59_RS09415 - 2039342..2040037 (-) 696 WP_286236660.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUD59_RS09420 pilT 2040127..2041161 (+) 1035 WP_286236662.1 type IV pilus twitching motility protein PilT Machinery gene
  QUD59_RS09425 pilU 2041182..2042348 (+) 1167 WP_286236663.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUD59_RS09430 - 2042422..2043072 (-) 651 WP_286236664.1 FecR family protein -
  QUD59_RS09440 smpB 2043581..2044054 (-) 474 WP_286236665.1 SsrA-binding protein SmpB -
  QUD59_RS09445 - 2044323..2045687 (+) 1365 WP_286236668.1 sodium-dependent transporter -
  QUD59_RS09450 - 2045704..2046135 (+) 432 WP_286236670.1 type II toxin-antitoxin system RatA family toxin -
  QUD59_RS09455 - 2046145..2046456 (+) 312 WP_286236671.1 RnfH family protein -
  QUD59_RS09460 bamE 2046460..2046798 (-) 339 WP_286236673.1 outer membrane protein assembly factor BamE -
  QUD59_RS09465 fur 2046914..2047336 (+) 423 WP_286236674.1 ferric iron uptake transcriptional regulator -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43594.70 Da        Isoelectric Point: 5.5846

>NTDB_id=100621 QUD59_RS09425 WP_286236663.1 2041182..2042348(+) (pilU) [Neptuniibacter halophilus strain LMG 25378]
MDFTQLLKVMVERDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQSRALVYSTMNDKQLAEFEGTHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKISDYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYVHDHKQSIITQREVGIDTESFEVALKNTLRQAPDVILMGEIRTKDTMQHGLVFAETGHLTLATLHANNANQALDRI
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKEVVKKSTNLGMQTFDQA
LFELYKDGVITYEVALAHADSANDLRLLIKLSADTNPELADQMEEQSFQMQEEDDFLHNEDDGQFKPR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=100621 QUD59_RS09425 WP_286236663.1 2041182..2042348(+) (pilU) [Neptuniibacter halophilus strain LMG 25378]
GTGGATTTTACGCAACTATTGAAAGTGATGGTGGAGCGGGACGCATCCGACTTGTTTGTGACCGCCGGGGCCCGGCCGAC
GATCAAAGTGGACGGAACACTGAAGCCTCTGACCAAGGATACTCTCAAACCTTCCCAGTCACGGGCGCTGGTTTACAGCA
CAATGAATGACAAACAACTGGCCGAGTTCGAAGGCACTCATGAGTGTAACTTCGCGATCAGCGCGCCAGGGCTAGGGCGA
TTTCGTGTCTCGGCATTTTTTCAGCGCAACTCTGCAGGGATGGTACTGAGGAAAATCAGTGACTATATCCCCAGTCTGGA
AGAGCTGAATCTGCCGCCGATTCTGCGTGATCTTTCGATGACCAAGCGTGGTCTGATCCTGTTTGTGGGGGGAACCTCGA
CGGGTAAATCGACCTCACTGGCATCCATGATCGACTACCGAAATACCAATCACCGTGGTCATATCATTACCATTGAAGAC
CCGATTGAGTACGTACACGATCACAAGCAGAGTATTATCACGCAGCGTGAAGTGGGGATTGATACCGAATCCTTCGAAGT
GGCCTTGAAAAACACCCTGCGTCAGGCGCCTGACGTCATCCTGATGGGTGAGATCCGAACCAAGGACACCATGCAGCACG
GTCTTGTATTCGCAGAGACCGGCCACCTTACGCTGGCAACCCTGCACGCTAACAACGCCAACCAGGCGTTGGACCGTATA
ATCAGCTTCTTCCCGCCGGAGCATCACGACCAGCTATGGATGGACCTGTCGCTGAACCTTAAGGCGATTATCGCCCAGCA
ACTGTTGCCGACCAAAGATGGTAAAGGCCGTCGTGCCGCCATCGAAGTGCTGATCAACACCCCACTGATTCAGGATCTGA
TCCGTAAAGGTGAAGTGCACGAGATCAAAGAGGTAGTGAAGAAATCCACCAACCTGGGGATGCAGACCTTCGATCAGGCG
CTGTTTGAGCTCTATAAAGATGGCGTTATTACCTACGAGGTTGCGCTGGCCCATGCGGATTCTGCCAACGACCTGCGCCT
GCTGATCAAGCTGAGTGCGGATACGAATCCGGAACTGGCTGACCAGATGGAAGAGCAGTCCTTCCAGATGCAGGAAGAGG
ATGATTTTCTTCACAACGAAGATGATGGCCAGTTTAAGCCACGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.6

96.649

0.634
  pilU Acinetobacter baylyi ADP1

58.701

99.227

0.582
  pilU Vibrio cholerae strain A1552

55.714

90.206

0.503
  pilT Acinetobacter baylyi ADP1

44.282

87.887

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.658

87.371

0.381
  pilT Acinetobacter baumannii D1279779

43.582

86.34

0.376
  pilT Acinetobacter nosocomialis M2

43.582

86.34

0.376
  pilT Acinetobacter baumannii strain A118

43.582

86.34

0.376
  pilT Pseudomonas aeruginosa PAK

42.985

86.34

0.371
  pilT Pseudomonas stutzeri DSM 10701

42.388

86.34

0.366
  pilT Legionella pneumophila strain Lp02

41.791

86.34

0.361
  pilT Legionella pneumophila strain ERS1305867

41.791

86.34

0.361

Multiple sequence alignment