Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABI149_RS14100 Genome accession   NZ_CP157249
Coordinates   2962576..2963613 (+) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain 2024CK-00376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2959584..2988103 2962576..2963613 within 0


Gene organization within MGE regions


Location: 2959584..2988103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABI149_RS14095 (ABI149_14095) - 2961757..2962449 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABI149_RS14100 (ABI149_14100) pilT 2962576..2963613 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  ABI149_RS14105 (ABI149_14105) pilU 2963641..2964759 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABI149_RS14110 (ABI149_14110) fur 2964829..2965266 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  ABI149_RS14115 (ABI149_14115) bamE 2965378..2965776 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  ABI149_RS14120 (ABI149_14120) - 2965831..2966154 (-) 324 WP_000442587.1 RnfH family protein -
  ABI149_RS14125 (ABI149_14125) - 2966154..2967221 (-) 1068 WP_002019572.1 hypothetical protein -
  ABI149_RS14130 (ABI149_14130) - 2967374..2967829 (-) 456 WP_000782976.1 bacteriohemerythrin -
  ABI149_RS14135 (ABI149_14135) - 2968082..2968930 (-) 849 WP_000336548.1 class II glutamine amidotransferase -
  ABI149_RS14140 (ABI149_14140) - 2969039..2969821 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  ABI149_RS14145 (ABI149_14145) argB 2969942..2970850 (-) 909 WP_001135419.1 acetylglutamate kinase -
  ABI149_RS14150 (ABI149_14150) - 2970865..2972283 (-) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  ABI149_RS14155 (ABI149_14155) dut 2972483..2972935 (-) 453 WP_000868152.1 dUTP diphosphatase -
  ABI149_RS14160 (ABI149_14160) rep 2972960..2974999 (-) 2040 WP_000093035.1 DNA helicase Rep -
  ABI149_RS14165 (ABI149_14165) - 2975193..2975846 (-) 654 WP_001202415.1 OmpA family protein -
  ABI149_RS14170 (ABI149_14170) - 2976091..2977017 (+) 927 WP_000100965.1 acyltransferase -
  ABI149_RS14175 (ABI149_14175) - 2977034..2978080 (+) 1047 WP_001181639.1 hypothetical protein -
  ABI149_RS14180 (ABI149_14180) minC 2978202..2978924 (+) 723 WP_000763677.1 septum site-determining protein MinC -
  ABI149_RS14185 (ABI149_14185) minD 2978995..2979807 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  ABI149_RS14190 (ABI149_14190) minE 2979810..2980082 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  ABI149_RS14195 (ABI149_14195) - 2980166..2980453 (+) 288 WP_001218560.1 PA4642 family protein -
  ABI149_RS14200 (ABI149_14200) - 2980601..2980918 (+) 318 WP_216076828.1 hypothetical protein -
  ABI149_RS14205 (ABI149_14205) - 2980870..2981127 (+) 258 Protein_2784 hypothetical protein -
  ABI149_RS14210 (ABI149_14210) - 2981134..2982102 (-) 969 WP_005804963.1 IS30 family transposase -
  ABI149_RS14215 (ABI149_14215) - 2982215..2982490 (+) 276 Protein_2786 tyrosyl-tRNA synthetase -
  ABI149_RS14220 (ABI149_14220) rhtC 2982546..2983166 (-) 621 WP_000959259.1 threonine export protein RhtC -
  ABI149_RS14225 (ABI149_14225) - 2983316..2986348 (-) 3033 WP_000127823.1 DNA translocase FtsK -
  ABI149_RS14230 (ABI149_14230) trxB 2986618..2987565 (+) 948 WP_001276144.1 thioredoxin-disulfide reductase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=1005082 ABI149_RS14100 WP_000355489.1 2962576..2963613(+) (pilT) [Acinetobacter baumannii strain 2024CK-00376]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1005082 ABI149_RS14100 WP_000355489.1 2962576..2963613(+) (pilT) [Acinetobacter baumannii strain 2024CK-00376]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCCGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CAGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTACACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTACTCAGCCATTCAAACTGGTGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGCCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment