Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABI149_RS14105 Genome accession   NZ_CP157249
Coordinates   2963641..2964759 (+) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain 2024CK-00376     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2959584..2988103 2963641..2964759 within 0


Gene organization within MGE regions


Location: 2959584..2988103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABI149_RS14095 (ABI149_14095) - 2961757..2962449 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABI149_RS14100 (ABI149_14100) pilT 2962576..2963613 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  ABI149_RS14105 (ABI149_14105) pilU 2963641..2964759 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABI149_RS14110 (ABI149_14110) fur 2964829..2965266 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  ABI149_RS14115 (ABI149_14115) bamE 2965378..2965776 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  ABI149_RS14120 (ABI149_14120) - 2965831..2966154 (-) 324 WP_000442587.1 RnfH family protein -
  ABI149_RS14125 (ABI149_14125) - 2966154..2967221 (-) 1068 WP_002019572.1 hypothetical protein -
  ABI149_RS14130 (ABI149_14130) - 2967374..2967829 (-) 456 WP_000782976.1 bacteriohemerythrin -
  ABI149_RS14135 (ABI149_14135) - 2968082..2968930 (-) 849 WP_000336548.1 class II glutamine amidotransferase -
  ABI149_RS14140 (ABI149_14140) - 2969039..2969821 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  ABI149_RS14145 (ABI149_14145) argB 2969942..2970850 (-) 909 WP_001135419.1 acetylglutamate kinase -
  ABI149_RS14150 (ABI149_14150) - 2970865..2972283 (-) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  ABI149_RS14155 (ABI149_14155) dut 2972483..2972935 (-) 453 WP_000868152.1 dUTP diphosphatase -
  ABI149_RS14160 (ABI149_14160) rep 2972960..2974999 (-) 2040 WP_000093035.1 DNA helicase Rep -
  ABI149_RS14165 (ABI149_14165) - 2975193..2975846 (-) 654 WP_001202415.1 OmpA family protein -
  ABI149_RS14170 (ABI149_14170) - 2976091..2977017 (+) 927 WP_000100965.1 acyltransferase -
  ABI149_RS14175 (ABI149_14175) - 2977034..2978080 (+) 1047 WP_001181639.1 hypothetical protein -
  ABI149_RS14180 (ABI149_14180) minC 2978202..2978924 (+) 723 WP_000763677.1 septum site-determining protein MinC -
  ABI149_RS14185 (ABI149_14185) minD 2978995..2979807 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  ABI149_RS14190 (ABI149_14190) minE 2979810..2980082 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  ABI149_RS14195 (ABI149_14195) - 2980166..2980453 (+) 288 WP_001218560.1 PA4642 family protein -
  ABI149_RS14200 (ABI149_14200) - 2980601..2980918 (+) 318 WP_216076828.1 hypothetical protein -
  ABI149_RS14205 (ABI149_14205) - 2980870..2981127 (+) 258 Protein_2784 hypothetical protein -
  ABI149_RS14210 (ABI149_14210) - 2981134..2982102 (-) 969 WP_005804963.1 IS30 family transposase -
  ABI149_RS14215 (ABI149_14215) - 2982215..2982490 (+) 276 Protein_2786 tyrosyl-tRNA synthetase -
  ABI149_RS14220 (ABI149_14220) rhtC 2982546..2983166 (-) 621 WP_000959259.1 threonine export protein RhtC -
  ABI149_RS14225 (ABI149_14225) - 2983316..2986348 (-) 3033 WP_000127823.1 DNA translocase FtsK -
  ABI149_RS14230 (ABI149_14230) trxB 2986618..2987565 (+) 948 WP_001276144.1 thioredoxin-disulfide reductase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=1005083 ABI149_RS14105 WP_000347039.1 2963641..2964759(+) (pilU) [Acinetobacter baumannii strain 2024CK-00376]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1005083 ABI149_RS14105 WP_000347039.1 2963641..2964759(+) (pilU) [Acinetobacter baumannii strain 2024CK-00376]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395

Multiple sequence alignment