Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABIS04_RS05205 Genome accession   NZ_CP157213
Coordinates   1181574..1182611 (+) Length   345 a.a.
NCBI ID   WP_140233637.1    Uniprot ID   A0A4Y5YD10
Organism   Shewanella sp. H8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1176574..1187611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABIS04_RS05175 (ABIS04_05175) rdgB 1177173..1177826 (-) 654 WP_350432800.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ABIS04_RS05180 (ABIS04_05180) - 1178199..1178660 (-) 462 WP_350432801.1 DUF4426 domain-containing protein -
  ABIS04_RS05185 (ABIS04_05185) yggU 1178746..1179045 (-) 300 WP_137222182.1 DUF167 family protein YggU -
  ABIS04_RS05190 (ABIS04_05190) - 1179066..1179614 (-) 549 WP_137222180.1 YggT family protein -
  ABIS04_RS05195 (ABIS04_05195) proC 1179809..1180627 (-) 819 WP_350432802.1 pyrroline-5-carboxylate reductase -
  ABIS04_RS05200 (ABIS04_05200) - 1180860..1181546 (-) 687 WP_350432803.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABIS04_RS05205 (ABIS04_05205) pilT 1181574..1182611 (+) 1038 WP_140233637.1 type IV pilus twitching motility protein PilT Machinery gene
  ABIS04_RS05210 (ABIS04_05210) pilU 1182623..1183735 (+) 1113 WP_350432804.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABIS04_RS05215 (ABIS04_05215) - 1183984..1185087 (+) 1104 WP_350432805.1 hypothetical protein -
  ABIS04_RS05220 (ABIS04_05220) - 1185193..1185726 (+) 534 WP_350432806.1 glutathione peroxidase -
  ABIS04_RS05225 (ABIS04_05225) hemH 1185955..1186968 (+) 1014 WP_350432807.1 ferrochelatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38373.95 Da        Isoelectric Point: 6.9094

>NTDB_id=1004691 ABIS04_RS05205 WP_140233637.1 1181574..1182611(+) (pilT) [Shewanella sp. H8]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAGAVFRTIPSDILSLEQLGAPDIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLDQCL
QNLVNRGLITREDAMQKSSNKQTNF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1004691 ABIS04_RS05205 WP_140233637.1 1181574..1182611(+) (pilT) [Shewanella sp. H8]
ATGGAAATCACTGAATTACTCGCCTTTAGTGTAAAACACAAAGCATCAGATCTACATCTTTCAGCGGGCGTGTCCCCGAT
GATCCGTGTTGATGGTGAGGTGAGAAAAATTAACTTACCTGCATTAGATCATCAAGGCGTTCATAGTCTTGTATATGACA
TCATGAATGATAAACAACGTAAAGACTATGAAGAACATCTTGAAATCGATTTTTCATTTGAAGTCCCTAACCTAGCACGT
TTTCGTGTTAACGCATTCAATCAGTCTCGTGGTGCTGGCGCCGTATTTCGTACTATTCCGTCAGACATTTTGTCACTTGA
GCAACTTGGCGCACCAGACATTTTTAAGAAAATTGCCAGCTTTCCTCGTGGGTTAGTCTTAGTTACTGGGCCTACAGGCT
CAGGTAAATCAACCACCTTGGCAGCAATGGTAGATTACGTTAATGAAAATCGACACGATCATATTTTAACCATTGAAGAC
CCAATCGAATTCGTTCATCAGAACAAACAATGCTTAATCAACCAGCGTGAAGTTCATAAGCATACCCATAGCTTTAACGC
TGCACTGAGAAGTGCATTACGTGAAGACCCGGATGTTATTCTTGTAGGTGAAATGCGCGACCTTGAAACCATTCGTTTAG
CCATGACAGCCGCTGAAACGGGCCATTTAGTATTTGGTACCTTGCATACTACATCAGCCGCAAAAACCATTGACCGTGTG
GTCGATGTATTTCCCGAAGGTGAAAAGAGTATGGTTCGCACCATGCTGTCTGAATCACTTCAAGCGGTTATTTCGCAAAC
ACTGATCAAAAAAATTGGTGGTGGTCGAGTTGCCGCTCACGAAATTATGATGGGTACTCCAGCGATTCGAAATCTTATTC
GTGAAGATAAAGTCGCGCAAATGTATTCTGCCATTCAAACGGGTATGTCTCACGGTATGCAAACCCTTGACCAATGTCTG
CAAAACTTAGTCAATCGTGGTTTAATCACCCGTGAAGATGCAATGCAAAAAAGTTCGAATAAACAAACTAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y5YD10

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.391

100

0.774
  pilT Acinetobacter baylyi ADP1

79.939

95.362

0.762
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.56

97.391

0.414
  pilU Vibrio cholerae strain A1552

41.056

98.841

0.406
  pilU Acinetobacter baylyi ADP1

40.237

97.971

0.394

Multiple sequence alignment