Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABIS04_RS05210 Genome accession   NZ_CP157213
Coordinates   1182623..1183735 (+) Length   370 a.a.
NCBI ID   WP_350432804.1    Uniprot ID   -
Organism   Shewanella sp. H8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1177623..1188735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABIS04_RS05180 (ABIS04_05180) - 1178199..1178660 (-) 462 WP_350432801.1 DUF4426 domain-containing protein -
  ABIS04_RS05185 (ABIS04_05185) yggU 1178746..1179045 (-) 300 WP_137222182.1 DUF167 family protein YggU -
  ABIS04_RS05190 (ABIS04_05190) - 1179066..1179614 (-) 549 WP_137222180.1 YggT family protein -
  ABIS04_RS05195 (ABIS04_05195) proC 1179809..1180627 (-) 819 WP_350432802.1 pyrroline-5-carboxylate reductase -
  ABIS04_RS05200 (ABIS04_05200) - 1180860..1181546 (-) 687 WP_350432803.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABIS04_RS05205 (ABIS04_05205) pilT 1181574..1182611 (+) 1038 WP_140233637.1 type IV pilus twitching motility protein PilT Machinery gene
  ABIS04_RS05210 (ABIS04_05210) pilU 1182623..1183735 (+) 1113 WP_350432804.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABIS04_RS05215 (ABIS04_05215) - 1183984..1185087 (+) 1104 WP_350432805.1 hypothetical protein -
  ABIS04_RS05220 (ABIS04_05220) - 1185193..1185726 (+) 534 WP_350432806.1 glutathione peroxidase -
  ABIS04_RS05225 (ABIS04_05225) hemH 1185955..1186968 (+) 1014 WP_350432807.1 ferrochelatase -
  ABIS04_RS05230 (ABIS04_05230) yciH 1187326..1187655 (+) 330 WP_350432808.1 stress response translation initiation inhibitor YciH -
  ABIS04_RS05235 (ABIS04_05235) - 1187689..1188249 (+) 561 WP_350432809.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41109.07 Da        Isoelectric Point: 6.0920

>NTDB_id=1004692 ABIS04_RS05210 WP_350432804.1 1182623..1183735(+) (pilU) [Shewanella sp. H8]
MDVRPFLNIMVEKKASDLFVTAGFPPSAKIDGELRQLSENRLTPEQSLVFVESLMSDAQKKEFHDTSECNFAFAAKDLGR
FRVSAFWQRESAGCVMRRIETQIPEVDDLKLPAILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLIPKSDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMGKSREQGMQTFDQA
LLDLYVEGEISYADALHHADSPNDLRLMIKLQSNETTSTGIMEGITFDKD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1004692 ABIS04_RS05210 WP_350432804.1 1182623..1183735(+) (pilU) [Shewanella sp. H8]
ATGGATGTTCGTCCATTTTTAAATATCATGGTTGAGAAAAAGGCGTCTGATTTATTTGTCACCGCAGGTTTTCCGCCCAG
TGCAAAAATTGATGGTGAATTACGTCAACTTTCTGAAAATCGATTAACACCAGAGCAATCGTTGGTGTTTGTTGAATCGT
TGATGAGTGATGCGCAAAAGAAAGAGTTTCACGACACCAGTGAATGTAACTTTGCTTTTGCGGCAAAAGATTTAGGGCGC
TTTCGTGTCAGTGCATTTTGGCAACGTGAATCAGCTGGCTGCGTAATGCGTCGTATTGAAACTCAAATTCCAGAAGTCGA
TGACTTAAAACTGCCTGCTATTCTCAAAGATTTGGTCATGAGTAAACGTGGTCTTATCATTATGGTCGGCGGAACGGGTA
CTGGTAAGTCAACATCGTTGGCTGCACTAGTGGGTTACCGAAACGCGCATGCCCGTGGGCATATCCTTACTATTGAAGAC
CCAGTTGAGTTTGTTCATAACCATCGTAAAAGTATTATTACTCAACGAGAAGTGGGTATTGATACTGAGTCATTTGATGC
CGCACTTAAAAGCTCGTTACGCCAAGCACCAGATGTGATCCTAATTGGTGAAATCCGCACTCAAGAAACCATGGAGTTTG
CGCTGTCTTTTGCTGAAACGGGTCATTTATGCATGGCGACCTTGCATGCTAACAATGCTAACCAAGCACTTGATCGCATC
ATGCATTTAGTGCCAGAAAGTAAGCACAATCAACTCTTATTCGATTTGTCATTGAACTTACGCGGCATTGTCGCGCAACA
GCTTATACCTAAATCAGACGGAACCGGCCGTCGCGCCGCCATTGAAGTGTTAATTAATACCCCTCGCGTGGCCAGTTTGA
TTGCTAAAAATGAGTTGCATTTACTAAAAGAAACCATGGGTAAGTCACGTGAACAAGGCATGCAAACGTTCGACCAAGCT
TTGTTAGATTTATATGTAGAGGGTGAAATTAGTTATGCCGATGCATTACACCATGCTGACTCACCTAATGATTTACGCTT
AATGATTAAATTACAGAGTAACGAAACGACCTCTACGGGAATAATGGAAGGAATTACTTTCGATAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Acinetobacter baylyi ADP1

59.943

95.135

0.57
  pilU Vibrio cholerae strain A1552

57.065

99.459

0.568
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362

Multiple sequence alignment