Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAG688_RS13755 Genome accession   NZ_CP157201
Coordinates   2972925..2973962 (-) Length   345 a.a.
NCBI ID   WP_411832798.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain SDU_PSEU2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2967925..2978962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG688_RS13735 - 2969419..2970363 (-) 945 WP_411832793.1 aspartate carbamoyltransferase catalytic subunit -
  AAG688_RS13740 ruvX 2970410..2970862 (-) 453 WP_411832794.1 Holliday junction resolvase RuvX -
  AAG688_RS13745 - 2970948..2971514 (-) 567 WP_411832795.1 YqgE/AlgH family protein -
  AAG688_RS13750 pilU 2971669..2972799 (-) 1131 WP_411832797.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG688_RS13755 pilT 2972925..2973962 (-) 1038 WP_411832798.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG688_RS13760 - 2974056..2974742 (+) 687 WP_411832799.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAG688_RS13765 proC 2974990..2975838 (+) 849 WP_411832800.1 pyrroline-5-carboxylate reductase -
  AAG688_RS13770 - 2975850..2976305 (+) 456 WP_411832801.1 DUF4426 domain-containing protein -
  AAG688_RS13775 - 2976361..2977338 (-) 978 WP_411832802.1 PA2778 family cysteine peptidase -
  AAG688_RS13780 - 2977338..2977706 (-) 369 WP_411832803.1 PA2779 family protein -
  AAG688_RS13785 - 2977775..2978650 (-) 876 WP_411832804.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38532.26 Da        Isoelectric Point: 6.7271

>NTDB_id=1004581 AAG688_RS13755 WP_411832798.1 2972925..2973962(-) (pilT) [Pseudoxanthomonas mexicana strain SDU_PSEU2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPLFRELIAQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
IDVFPAGEKPMVRSMLSESLRAVISQALLKKIGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQNGMMTLDQHL
QDLVKRALVTRQQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1004581 AAG688_RS13755 WP_411832798.1 2972925..2973962(-) (pilT) [Pseudoxanthomonas mexicana strain SDU_PSEU2]
ATGGATATCGCCGAACTTCTGGCGTTCTCGGTCAAGAACAAAGCCTCGGACCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATCCGGGTGGACGGCGACGTGCGCCGCATCAACATCCCGGCGCTGGACCACAAGCAGGTCCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATCCCGTCGCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGTGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGACCCTGGA
GGACCTGGGTTGCCCGCCGCTGTTCCGCGAACTCATCGCACAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCAGCGTCGAGGAT
CCGATCGAGTTCGTGCACACTTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTCCACCGCGACACTCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAGGATCCGGACTACATCCTGGTTGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCCGAGACCGGCCACCTGGTGTTCGCCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCGTG
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGGC
CCTGCTGAAGAAGATCGGCGGCGGCCGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGAACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCGCCCTGGTCACCCGCCAGCAGGCCAAGGAATACGCCAAGGACAAGCGGTTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Acinetobacter baylyi ADP1

76.163

99.71

0.759
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.657

97.101

0.667
  pilT Vibrio cholerae strain A1552

68.657

97.101

0.667
  pilT Neisseria meningitidis 8013

63.93

98.841

0.632
  pilT Neisseria gonorrhoeae MS11

63.636

98.841

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Vibrio cholerae strain A1552

39.655

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment