Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAG688_RS13750 Genome accession   NZ_CP157201
Coordinates   2971669..2972799 (-) Length   376 a.a.
NCBI ID   WP_411832797.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain SDU_PSEU2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2966669..2977799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG688_RS13730 - 2967492..2969114 (+) 1623 WP_411832792.1 GGDEF domain-containing protein -
  AAG688_RS13735 - 2969419..2970363 (-) 945 WP_411832793.1 aspartate carbamoyltransferase catalytic subunit -
  AAG688_RS13740 ruvX 2970410..2970862 (-) 453 WP_411832794.1 Holliday junction resolvase RuvX -
  AAG688_RS13745 - 2970948..2971514 (-) 567 WP_411832795.1 YqgE/AlgH family protein -
  AAG688_RS13750 pilU 2971669..2972799 (-) 1131 WP_411832797.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG688_RS13755 pilT 2972925..2973962 (-) 1038 WP_411832798.1 type IV pilus twitching motility protein PilT Machinery gene
  AAG688_RS13760 - 2974056..2974742 (+) 687 WP_411832799.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAG688_RS13765 proC 2974990..2975838 (+) 849 WP_411832800.1 pyrroline-5-carboxylate reductase -
  AAG688_RS13770 - 2975850..2976305 (+) 456 WP_411832801.1 DUF4426 domain-containing protein -
  AAG688_RS13775 - 2976361..2977338 (-) 978 WP_411832802.1 PA2778 family cysteine peptidase -
  AAG688_RS13780 - 2977338..2977706 (-) 369 WP_411832803.1 PA2779 family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41878.21 Da        Isoelectric Point: 6.8286

>NTDB_id=1004580 AAG688_RS13750 WP_411832797.1 2971669..2972799(-) (pilU) [Pseudoxanthomonas mexicana strain SDU_PSEU2]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSIKVHGKISPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDTWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLIQDYIREGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGNASTLAQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1004580 AAG688_RS13750 WP_411832797.1 2971669..2972799(-) (pilU) [Pseudoxanthomonas mexicana strain SDU_PSEU2]
ATGAGCACCATAGATTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATCAAGGTGCACGGCAAGATCTCGCCGATCACGCAGACGCCGTTGACCTCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCATGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTGGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACCAGGTCGGCATGGTCCTGCGCCGCATCGAGACGCGCATCCC
GACCATCGAGGAGCTGAACCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAC
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCCCCCGACGTCATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGATCCCCACCCCGGACGGCAAGGGCCGCCGCGTGGCGATGGAAATCATGCTCGGCACCCCGCTGATCC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCTCCTACGAGGACGCCCTGCGCTACGCCGACTCCCAGAACGA
GGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCAACGCCAGCACGCTGGCGCAGGGGCTGGATGGGGTGGAAGTGGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.933

94.681

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.265

96.277

0.407
  pilT Acinetobacter baumannii strain A118

41.279

91.489

0.378
  pilT Acinetobacter nosocomialis M2

41.279

91.489

0.378
  pilT Acinetobacter baumannii D1279779

41.279

91.489

0.378
  pilT Pseudomonas aeruginosa PAK

42.09

89.096

0.375
  pilT Legionella pneumophila strain Lp02

41.194

89.096

0.367
  pilT Legionella pneumophila strain ERS1305867

41.194

89.096

0.367

Multiple sequence alignment