Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABHF54_RS12545 Genome accession   NZ_CP157069
Coordinates   2625421..2626464 (-) Length   347 a.a.
NCBI ID   WP_276679084.1    Uniprot ID   -
Organism   Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2620421..2631464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF54_RS12530 (ABHF54_12525) - 2621158..2622870 (-) 1713 WP_011111571.1 hydroxylamine oxidoreductase -
  ABHF54_RS12535 (ABHF54_12530) mnmA 2623177..2624265 (+) 1089 WP_347888249.1 tRNA 2-thiouridine(34) synthase MnmA -
  ABHF54_RS12540 (ABHF54_12535) pilU 2624268..2625404 (-) 1137 WP_287398850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHF54_RS12545 (ABHF54_12540) pilT 2625421..2626464 (-) 1044 WP_276679084.1 type IV pilus twitching motility protein PilT Machinery gene
  ABHF54_RS12550 (ABHF54_12545) - 2626661..2627377 (+) 717 WP_011111575.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABHF54_RS12555 (ABHF54_12550) - 2627346..2627615 (-) 270 WP_276679088.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ABHF54_RS12560 (ABHF54_12555) coaD 2627635..2628114 (-) 480 WP_347888250.1 pantetheine-phosphate adenylyltransferase -
  ABHF54_RS12565 (ABHF54_12560) rsmD 2628107..2628658 (-) 552 WP_347889080.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ABHF54_RS12570 (ABHF54_12565) - 2628668..2629972 (-) 1305 WP_347888251.1 pitrilysin family protein -
  ABHF54_RS12575 (ABHF54_12570) - 2630127..2630546 (+) 420 WP_011111580.1 VOC family protein -
  ABHF54_RS12580 (ABHF54_12575) - 2630577..2630975 (+) 399 WP_324520915.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38453.23 Da        Isoelectric Point: 7.1151

>NTDB_id=1003943 ABHF54_RS12545 WP_276679084.1 2625421..2626464(-) (pilT) [Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf]
MNIVELLSFVVKNNASDLHLSAGMPPMIRVHGEIRRINLPALEHKDVHDMVYDIMNDSQRKHYEEHLECDFSFAIPDLAR
FRVNAFNTQRGAASVLRTIPSRVLTLEELKAPKIFAEIAQQPRGVVLVTGPTGSGKSTTLAAMINDINENQYGHILTLED
PIEFVHESKKCLINQREVGRDTHSFSNALRAALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLRAVISQALLKTKDGKGRVAAHEIMIGTPAIRNLIREGKVAQMYSAIQTGQGVGMQTLDQN
LTDLVKRGVISAAEARTKAMNKDNFRG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1003943 ABHF54_RS12545 WP_276679084.1 2625421..2626464(-) (pilT) [Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf]
ATGAATATAGTAGAGTTACTCTCATTTGTGGTCAAGAATAACGCTTCCGACCTTCATTTGTCTGCCGGTATGCCGCCTAT
GATCCGGGTGCACGGGGAAATCCGGCGCATCAATCTCCCTGCGCTGGAGCACAAAGACGTGCATGACATGGTGTATGACA
TTATGAACGATAGTCAGCGCAAGCATTATGAAGAACATCTGGAGTGCGATTTTTCGTTTGCTATCCCCGATCTGGCACGT
TTTCGTGTCAACGCTTTCAATACTCAGCGGGGAGCAGCTTCGGTGCTGCGTACCATTCCGTCCAGAGTGCTGACACTCGA
AGAACTGAAGGCGCCAAAAATATTTGCTGAAATTGCGCAACAACCACGCGGTGTGGTGCTTGTTACGGGACCGACCGGAT
CCGGTAAATCCACCACGCTTGCAGCCATGATCAATGACATCAATGAGAACCAGTATGGCCACATACTGACTTTGGAGGAT
CCGATCGAATTTGTACACGAAAGCAAGAAATGTCTGATCAATCAGCGGGAGGTCGGGCGAGATACACACAGTTTCAGCAA
TGCTCTGCGTGCGGCTTTGAGGGAAGATCCGGACATCATTCTGGTGGGCGAAATGCGGGATCTGGAGACAATTCGTCTGG
CCATGACTGCGGCGGAAACCGGTCATCTGGTTTTTGGTACGCTGCATACCAGTTCGGCTGCCAAGACCATCGACAGGATT
ATTGACGTGTTTCCGGCGGAGGAGAAGGAAATGGTGCGTGCGATGCTGTCCGAATCACTGCGTGCCGTTATTTCTCAGGC
ACTGCTGAAAACAAAGGATGGTAAGGGTCGGGTGGCTGCTCATGAAATCATGATCGGTACACCGGCTATCCGTAACCTTA
TCCGGGAAGGAAAAGTAGCGCAAATGTATTCGGCTATCCAGACTGGTCAGGGAGTCGGGATGCAAACACTCGATCAGAAT
CTGACAGATCTGGTGAAGCGTGGCGTCATTTCAGCGGCAGAAGCGCGTACCAAAGCTATGAACAAAGATAATTTCCGTGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas aeruginosa PAK

73.623

99.424

0.732
  pilT Pseudomonas stutzeri DSM 10701

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.935

97.406

0.671
  pilT Vibrio cholerae strain A1552

68.935

97.406

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.775

99.712

0.427
  pilU Pseudomonas stutzeri DSM 10701

42.197

99.712

0.421
  pilU Acinetobacter baylyi ADP1

40.922

100

0.409

Multiple sequence alignment