Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABHF54_RS12540 Genome accession   NZ_CP157069
Coordinates   2624268..2625404 (-) Length   378 a.a.
NCBI ID   WP_287398850.1    Uniprot ID   A0A7S6LZ43
Organism   Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2619268..2630404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF54_RS12520 (ABHF54_12515) cycA 2619289..2619996 (-) 708 WP_011111569.1 cytochrome c-550 CycA -
  ABHF54_RS12525 (ABHF54_12520) haoB 2620052..2621161 (-) 1110 WP_347888194.1 hydroxylamine oxidation protein HaoB -
  ABHF54_RS12530 (ABHF54_12525) - 2621158..2622870 (-) 1713 WP_011111571.1 hydroxylamine oxidoreductase -
  ABHF54_RS12535 (ABHF54_12530) mnmA 2623177..2624265 (+) 1089 WP_347888249.1 tRNA 2-thiouridine(34) synthase MnmA -
  ABHF54_RS12540 (ABHF54_12535) pilU 2624268..2625404 (-) 1137 WP_287398850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHF54_RS12545 (ABHF54_12540) pilT 2625421..2626464 (-) 1044 WP_276679084.1 type IV pilus twitching motility protein PilT Machinery gene
  ABHF54_RS12550 (ABHF54_12545) - 2626661..2627377 (+) 717 WP_011111575.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABHF54_RS12555 (ABHF54_12550) - 2627346..2627615 (-) 270 WP_276679088.1 YfhL family 4Fe-4S dicluster ferredoxin -
  ABHF54_RS12560 (ABHF54_12555) coaD 2627635..2628114 (-) 480 WP_347888250.1 pantetheine-phosphate adenylyltransferase -
  ABHF54_RS12565 (ABHF54_12560) rsmD 2628107..2628658 (-) 552 WP_347889080.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ABHF54_RS12570 (ABHF54_12565) - 2628668..2629972 (-) 1305 WP_347888251.1 pitrilysin family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42067.49 Da        Isoelectric Point: 6.8689

>NTDB_id=1003942 ABHF54_RS12540 WP_287398850.1 2624268..2625404(-) (pilU) [Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf]
MEKEQALKFMHDLLRLMLSKKASDLFITAGYPPAMKIDGKMTPVTQQPLSSAHTAALARAIMNDKQAVEFESSKECNFAI
HPEGIGRFRVNTFVQQQRTGIVLRTITTKIPNFDDLGLPQVLKEVVMSKRGLVIFVGGTGSGKSTSMAALIGHRNQNSHG
HIITIEDPVEFVHEHINCVVTQREVGVDTESWEAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCMGTLHANSA
NQALDRIINFFPEERRAQLLMDLSLNMRALVAQRLIPKKSGSGRAAAIEVMLNSPLISDLIFKGNVHEIKEIMKKSSELG
MQTFDMALFELYENGTISYEDALRNADSMNELRLQIKLHGSEARKISAETNIEHLTIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1003942 ABHF54_RS12540 WP_287398850.1 2624268..2625404(-) (pilU) [Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf]
ATGGAAAAAGAACAGGCACTCAAATTTATGCACGATTTACTGCGGCTGATGCTTAGTAAAAAGGCTTCAGATTTGTTCAT
TACGGCCGGTTATCCTCCGGCAATGAAGATAGATGGCAAAATGACACCTGTCACACAGCAGCCACTTTCTTCTGCCCATA
CTGCAGCGCTGGCGCGTGCCATCATGAACGATAAACAGGCGGTGGAGTTCGAGTCCAGCAAGGAATGTAATTTTGCAATT
CATCCGGAAGGAATCGGCCGTTTTCGCGTCAATACTTTCGTACAACAGCAACGAACAGGAATCGTTTTGCGGACGATCAC
CACGAAGATTCCAAATTTCGATGATCTTGGGCTGCCGCAGGTTCTGAAAGAAGTCGTCATGAGCAAACGTGGTCTGGTGA
TTTTTGTCGGTGGAACCGGCTCGGGAAAATCCACCTCGATGGCGGCACTGATTGGCCATCGCAACCAGAATAGCCATGGC
CATATCATCACTATTGAAGATCCGGTTGAATTTGTACATGAACATATCAATTGTGTCGTTACCCAGCGGGAAGTCGGAGT
TGATACGGAATCGTGGGAAGCTGCATTGAAGAATACATTGCGGCAGGCGCCCGATGTCATTCTGATTGGTGAAATCCGGG
ATCGAGAAACAATGGAGCACGCCATTGCTTTTGCTGAGACCGGGCATTTGTGTATGGGTACCCTGCATGCAAACAGCGCC
AATCAGGCGCTTGATCGCATTATTAATTTCTTCCCGGAAGAACGGCGTGCTCAGTTGCTGATGGACCTGTCACTGAATAT
GCGGGCATTGGTCGCGCAAAGGCTGATTCCCAAAAAATCAGGCAGCGGGCGTGCTGCGGCAATCGAAGTAATGCTCAATT
CTCCACTGATTTCTGATCTGATATTCAAGGGAAATGTGCATGAAATCAAGGAAATCATGAAAAAATCAAGTGAACTGGGG
ATGCAAACTTTTGATATGGCGCTGTTTGAACTCTATGAGAATGGCACGATTTCCTATGAAGATGCACTTCGTAATGCTGA
TTCCATGAATGAATTGAGGCTGCAAATCAAATTGCACGGATCAGAAGCCCGGAAAATCAGTGCAGAGACGAATATCGAAC
ATCTGACGATTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(19-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6LZ43

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.912

96.296

0.606
  pilU Acinetobacter baylyi ADP1

60.224

94.444

0.569
  pilU Vibrio cholerae strain A1552

56.891

90.212

0.513
  pilT Pseudomonas aeruginosa PAK

43.881

88.624

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.985

88.624

0.381
  pilT Legionella pneumophila strain Lp02

42.9

87.566

0.376
  pilT Legionella pneumophila strain ERS1305867

42.9

87.566

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.624

0.37

Multiple sequence alignment