Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFT82_RS19280 Genome accession   NZ_CP156749
Coordinates   3915216..3916250 (+) Length   344 a.a.
NCBI ID   WP_090380371.1    Uniprot ID   A0A1H4YTH5
Organism   Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3910216..3921250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT82_RS19255 (ABFT82_19255) - 3910481..3912463 (-) 1983 WP_347505710.1 dynamin-like GTPase family protein -
  ABFT82_RS19260 (ABFT82_19260) - 3912701..3912997 (-) 297 WP_090380360.1 DUF167 family protein -
  ABFT82_RS19265 (ABFT82_19265) - 3913011..3913601 (-) 591 WP_090380362.1 YggT family protein -
  ABFT82_RS19270 (ABFT82_19270) proC 3913611..3914429 (-) 819 WP_090380365.1 pyrroline-5-carboxylate reductase -
  ABFT82_RS19275 (ABFT82_19275) - 3914466..3915152 (-) 687 WP_090380368.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFT82_RS19280 (ABFT82_19280) pilT 3915216..3916250 (+) 1035 WP_090380371.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFT82_RS19285 (ABFT82_19285) pilU 3916357..3917502 (+) 1146 WP_090380374.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFT82_RS19290 (ABFT82_19290) - 3917468..3918346 (-) 879 WP_347505711.1 TIGR03915 family putative DNA repair protein -
  ABFT82_RS19295 (ABFT82_19295) - 3918346..3919557 (-) 1212 WP_347505712.1 putative DNA modification/repair radical SAM protein -
  ABFT82_RS19300 (ABFT82_19300) - 3919714..3919830 (+) 117 Protein_3785 TM2 domain-containing protein -
  ABFT82_RS19305 (ABFT82_19305) tnpB 3919878..3920273 (+) 396 WP_347505344.1 IS66 family insertion sequence element accessory protein TnpB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37959.66 Da        Isoelectric Point: 6.8361

>NTDB_id=1002525 ABFT82_RS19280 WP_090380371.1 3915216..3916250(+) (pilT) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLSMEDLGMGEIFRKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLVNQREVHRDTLGFTEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCL
KTLVTKGIVSREAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1002525 ABFT82_RS19280 WP_090380371.1 3915216..3916250(+) (pilT) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAGCAAGGTGCATCGGACCTGCATCTCTCCGCTGGCCTGCCTCCGAT
GATTCGGGTCGACGGTGATGTTCGCCGGATCAACCTGCCTGCGCTGGACCATAAACAGGTGCACGCACTGATTTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTCGAGACCGACTTCTCCTTCGAAGTCCCCGGCGTGGCGCGT
TTCCGGGTTAACGCCTTCAACCAGAATCGCGGCTCTGGCGCAGTATTCCGGACCATTCCGTCGAAAGTACTGAGCATGGA
AGACCTCGGTATGGGCGAGATCTTCCGCAAGATTTCCGATGTGCCGCGCGGCTTGGTGCTGGTGACAGGACCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACATCAACAGCAACAAGTATCACCACATCCTGACGGTCGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGGTCAACCAACGGGAAGTGCACCGTGACACTCTCGGCTTCACCGA
AGCCCTGCGCTCTGCGTTGCGCGAAGACCCGGACATCATCCTGGTCGGTGAGATGCGTGACCTGGAAACCATCCGCCTGG
CTCTGACCGCAGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGCGTG
GTTGACGTATTCCCAGCCGAAGAGAAGTCCATGGTTCGCTCCATGCTCTCCGAATCTCTGCAATCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGTGTGGCGGCCCATGAAATCATGATTGGCACTCCAGCCATTCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCTGCAATCCAGACTGGTGGCTCGCTGGGCATGCAGACCCTAGACTCCTGCCTG
AAAACTCTGGTGACCAAGGGCATTGTCAGTCGCGAAGCGGCTCGCGAGAAAGCCAAAACGCCAGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H4YTH5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Acinetobacter nosocomialis M2

84.593

100

0.846
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

82.558

100

0.826
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387

Multiple sequence alignment