Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFT82_RS19285 Genome accession   NZ_CP156749
Coordinates   3916357..3917502 (+) Length   381 a.a.
NCBI ID   WP_090380374.1    Uniprot ID   A0A1H4YR66
Organism   Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3911357..3922502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT82_RS19260 (ABFT82_19260) - 3912701..3912997 (-) 297 WP_090380360.1 DUF167 family protein -
  ABFT82_RS19265 (ABFT82_19265) - 3913011..3913601 (-) 591 WP_090380362.1 YggT family protein -
  ABFT82_RS19270 (ABFT82_19270) proC 3913611..3914429 (-) 819 WP_090380365.1 pyrroline-5-carboxylate reductase -
  ABFT82_RS19275 (ABFT82_19275) - 3914466..3915152 (-) 687 WP_090380368.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFT82_RS19280 (ABFT82_19280) pilT 3915216..3916250 (+) 1035 WP_090380371.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFT82_RS19285 (ABFT82_19285) pilU 3916357..3917502 (+) 1146 WP_090380374.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFT82_RS19290 (ABFT82_19290) - 3917468..3918346 (-) 879 WP_347505711.1 TIGR03915 family putative DNA repair protein -
  ABFT82_RS19295 (ABFT82_19295) - 3918346..3919557 (-) 1212 WP_347505712.1 putative DNA modification/repair radical SAM protein -
  ABFT82_RS19300 (ABFT82_19300) - 3919714..3919830 (+) 117 Protein_3785 TM2 domain-containing protein -
  ABFT82_RS19305 (ABFT82_19305) tnpB 3919878..3920273 (+) 396 WP_347505344.1 IS66 family insertion sequence element accessory protein TnpB -
  ABFT82_RS19310 (ABFT82_19310) - 3920292..3921848 (+) 1557 WP_090375563.1 IS66 family transposase -
  ABFT82_RS19315 (ABFT82_19315) - 3921885..3922184 (+) 300 Protein_3788 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42516.73 Da        Isoelectric Point: 6.6648

>NTDB_id=1002526 ABFT82_RS19285 WP_090380374.1 3916357..3917502(+) (pilU) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPITKTPMSPEQTRETVHSVMNEQQRRDFAENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDELKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGVDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKALMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLSSMAQGLSLEISEEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1002526 ABFT82_RS19285 WP_090380374.1 3916357..3917502(+) (pilU) [Pseudomonas anguilliseptica strain SICAU PA001 isolate largemouth bass]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTTATTACCGCTGGCGTACCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGATCACCAAAACGCCCATGTCGCCTGAGCAGACCCGGGAAACCGTGCATTCGG
TCATGAACGAGCAGCAGCGCCGCGACTTTGCCGAAAACCACGAATGCAACTTCGCCATCAGCGCCCGTGGTGTCGGTCGT
TTCCGCGTCAGTGCGTTCTATCAGCGCAACCTGGCCGGTATGGTGTTGCGTCGCATCGAGACCAATATCCCGACGCTGGA
CGAGCTGAAATTGCCGGAAGTACTGAAGAAACTTGCCCTGACCAAACGCGGTCTGGTGCTGTTCGTCGGTGCGACCGGTA
CCGGTAAGTCGACTTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCAATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCGTCGACACCGAGTCCTTCGAAGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATCGGTGAGGTGCGTACCCGCGAAACCATGGACCACG
CAGTGGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCTACGCTGCACGCCAACAACGCCAACCAAGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCGGATCGGCAGAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGATTCCGACTCCCGACGGCAAGGGGCGTCGCGCCGTAATCGAAGTGCTGATCAACACCCCGCTGGCCGCTGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGCGCTGATGAAGCGTTCTACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACAGCCAGGGCGAAATCACTTACGAAGACGCGCTGCTGTATGCGGATTCGGCCAACGACCTGCGCCT
GATGATCAAGCTTGGTTCGGAAACCGATGGCGATCACCTGAGCAGTATGGCCCAGGGCTTGTCGCTTGAAATTAGCGAAG
AGGATCCGGGTCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H4YR66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

91.601

100

0.916
  pilU Acinetobacter baylyi ADP1

63.866

93.701

0.598
  pilU Vibrio cholerae strain A1552

59.429

91.864

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.75

88.189

0.386
  pilT Pseudomonas aeruginosa PAK

41.791

87.927

0.367
  pilT Neisseria gonorrhoeae MS11

41.493

87.927

0.365
  pilT Neisseria meningitidis 8013

41.493

87.927

0.365

Multiple sequence alignment