Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFG87_RS08530 Genome accession   NZ_CP156074
Coordinates   1790397..1791440 (+) Length   347 a.a.
NCBI ID   WP_136772422.1    Uniprot ID   -
Organism   Chitiniphilus eburneus strain YS-30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1785397..1796440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFG87_RS08495 dsbD 1785495..1787036 (-) 1542 WP_136772429.1 protein-disulfide reductase DsbD -
  ABFG87_RS08500 cutA 1787076..1787405 (-) 330 WP_136772428.1 divalent-cation tolerance protein CutA -
  ABFG87_RS08505 - 1787404..1787826 (+) 423 WP_136772427.1 FxsA family protein -
  ABFG87_RS08510 - 1787865..1788170 (-) 306 WP_136772426.1 DUF167 domain-containing protein -
  ABFG87_RS08515 - 1788170..1788760 (-) 591 WP_136772425.1 YggT family protein -
  ABFG87_RS08520 proC 1788770..1789588 (-) 819 WP_136772424.1 pyrroline-5-carboxylate reductase -
  ABFG87_RS08525 - 1789585..1790304 (-) 720 WP_136772423.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFG87_RS08530 pilT 1790397..1791440 (+) 1044 WP_136772422.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFG87_RS08535 pilU 1791495..1792631 (+) 1137 WP_136772421.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFG87_RS08540 - 1792661..1794856 (+) 2196 WP_136772420.1 FimV family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38422.05 Da        Isoelectric Point: 6.6673

>NTDB_id=1001631 ABFG87_RS08530 WP_136772422.1 1790397..1791440(+) (pilT) [Chitiniphilus eburneus strain YS-30]
MEISELLAFAVKNKASDLHLSSGLPPMIRVHGDVRRINLPALEHKDVHDMVYDIMNDGQRKVYEDTLEADFSFEIPNLAR
FRVNAFVQQRGAGAVFRTIPSKVLTLEELNAPKVFQEISENPRGLVLVTGPTGSGKSTTLAAMVNYINENEYGHILTVED
PIEFVHQSKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGIPAIRNLIRENKIAQMYSSIQTGQQYGMQTLDQS
LQNLVQRNVVSIAEARTKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1001631 ABFG87_RS08530 WP_136772422.1 1790397..1791440(+) (pilT) [Chitiniphilus eburneus strain YS-30]
ATGGAAATTTCCGAACTCTTGGCCTTTGCCGTCAAGAACAAGGCATCCGACCTGCACCTTTCCTCCGGCCTGCCGCCCAT
GATCCGCGTTCACGGGGACGTGCGGCGGATCAACCTGCCCGCGCTCGAGCACAAGGATGTGCACGACATGGTGTACGACA
TCATGAACGACGGGCAACGCAAGGTTTACGAGGATACGCTGGAGGCGGACTTCTCGTTCGAGATCCCCAACCTGGCGCGT
TTCCGGGTCAATGCCTTCGTGCAGCAACGCGGCGCGGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTACTGACGCTCGA
GGAACTGAACGCCCCCAAGGTGTTCCAGGAAATCTCCGAAAACCCGCGCGGACTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAATCCACCACGCTGGCCGCGATGGTCAACTACATCAACGAAAACGAGTACGGACACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACCAGTCCAAGAAATGCCTGATCAACCAGCGTGAAGTCGGCCCGCATACCTTGAGCTTCTCCAA
CGCGCTGCGTTCCGCGCTGCGGGAAGACCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CGTTGACCGCCGCGGAAACGGGCCACCTTGTGTTTGGTACGCTGCACACCAGCTCGGCCGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCGGCAGCGGAAAAGGAGATGGTGCGTGCGATGCTGTCTGAATCGCTGCGCGCGGTGATCTCGCAAAC
GCTGCTCAAGACCAAGGACGGGCAGGGCCGCGTGGCGGCGCACGAGATCATGATCGGCATTCCGGCTATCCGTAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTATTCATCGATCCAGACCGGCCAGCAATACGGGATGCAGACGCTGGATCAGAGC
TTGCAGAACCTGGTGCAACGCAATGTTGTCTCGATCGCCGAGGCACGCACCAAGGCGGCCATCCCTGATAACTTCAAGGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.13

99.424

0.787
  pilT Acinetobacter baumannii D1279779

79.13

99.424

0.787
  pilT Acinetobacter baumannii strain A118

79.13

99.424

0.787
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Neisseria meningitidis 8013

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

73.451

97.695

0.718
  pilT Legionella pneumophila strain Lp02

73.716

95.389

0.703
  pilT Legionella pneumophila strain ERS1305867

73.716

95.389

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.406

0.663
  pilT Vibrio cholerae strain A1552

68.047

97.406

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Acinetobacter baylyi ADP1

38.905

100

0.389

Multiple sequence alignment