Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFG87_RS08535 Genome accession   NZ_CP156074
Coordinates   1791495..1792631 (+) Length   378 a.a.
NCBI ID   WP_136772421.1    Uniprot ID   A0A4V5MS03
Organism   Chitiniphilus eburneus strain YS-30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1786495..1797631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFG87_RS08500 cutA 1787076..1787405 (-) 330 WP_136772428.1 divalent-cation tolerance protein CutA -
  ABFG87_RS08505 - 1787404..1787826 (+) 423 WP_136772427.1 FxsA family protein -
  ABFG87_RS08510 - 1787865..1788170 (-) 306 WP_136772426.1 DUF167 domain-containing protein -
  ABFG87_RS08515 - 1788170..1788760 (-) 591 WP_136772425.1 YggT family protein -
  ABFG87_RS08520 proC 1788770..1789588 (-) 819 WP_136772424.1 pyrroline-5-carboxylate reductase -
  ABFG87_RS08525 - 1789585..1790304 (-) 720 WP_136772423.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFG87_RS08530 pilT 1790397..1791440 (+) 1044 WP_136772422.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFG87_RS08535 pilU 1791495..1792631 (+) 1137 WP_136772421.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFG87_RS08540 - 1792661..1794856 (+) 2196 WP_136772420.1 FimV family protein -
  ABFG87_RS08550 mltF 1795295..1796755 (+) 1461 WP_136772419.1 membrane-bound lytic murein transglycosylase MltF -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42134.33 Da        Isoelectric Point: 6.4349

>NTDB_id=1001632 ABFG87_RS08535 WP_136772421.1 1791495..1792631(+) (pilU) [Chitiniphilus eburneus strain YS-30]
MEKEQAAKFMQDLLRHMRAKGASDLFITADFPPAMKIDGKVTPVSAQVLTAQHTKELARSIMNDRQAEEFEATKECNFAI
SPGTLGRFRVNAFMQQSRVGMVLRTINSEIPRLSELGLPPVLQDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNESSYD
HIITIEDPIEYVHEHKNCIVTQREVGVDTDSWGAALKNTLRQAPDVILIGEIRDRDTMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRAQLLMDLSLNLKAFVSQRLLPHRSGKGRVAAVEVMLNSPLISELVFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLYEAGKVSYEDALRNADSVNDLRLQIKLQGSEAKQKDVLSGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1001632 ABFG87_RS08535 WP_136772421.1 1791495..1792631(+) (pilU) [Chitiniphilus eburneus strain YS-30]
ATGGAAAAGGAACAAGCAGCGAAATTCATGCAGGATCTCCTGCGGCATATGCGCGCCAAAGGCGCCTCCGATCTGTTTAT
TACCGCGGATTTTCCGCCCGCGATGAAGATCGACGGCAAGGTGACGCCGGTGTCGGCCCAGGTTCTGACCGCGCAGCACA
CCAAGGAGCTGGCCCGGTCGATCATGAATGACCGGCAGGCAGAGGAATTCGAAGCCACCAAGGAATGCAATTTCGCGATC
AGCCCCGGCACGCTCGGGCGTTTTCGCGTGAATGCCTTCATGCAGCAAAGCCGCGTGGGCATGGTGCTACGGACGATCAA
CTCCGAAATCCCGCGCCTGAGCGAGCTGGGCCTGCCGCCGGTATTGCAGGATATCGCCATGACCAAGCGTGGGCTGGTGA
TCTTCGTCGGTGGCACCGGCAGCGGCAAATCCACGTCGCTGGCCGCGATGATCGGCCATCGCAACGAAAGCTCGTACGAC
CACATCATCACCATCGAAGACCCGATCGAATACGTCCACGAGCACAAGAACTGCATCGTCACGCAACGGGAAGTGGGGGT
CGATACCGACTCCTGGGGCGCTGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ACCGCGACACCATGGACTACGCCATTGCCTTTGCCGAGACCGGTCACCTGTGCATGGCCACGCTGCATGCCAACTCGTCG
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGGCGCGCGCAGTTGCTGATGGATCTGTCGCTCAACCT
GAAAGCCTTCGTCTCGCAGCGCCTGTTGCCGCACCGTTCCGGCAAGGGCCGGGTGGCTGCGGTGGAGGTGATGCTCAACT
CGCCGTTGATTTCGGAGCTGGTGTTCAAGGGCGAAGTGCACGAGATCAAGGAAATCATGAAGAAATCTCGCGAACTGGGC
ATGCAGACCTTCGACCAGAGCCTGTTTGACCTGTACGAAGCAGGCAAGGTCAGCTATGAAGATGCGTTGCGCAATGCCGA
TTCCGTGAACGATCTACGCCTTCAGATCAAGCTGCAGGGTTCCGAAGCCAAGCAAAAGGACGTGTTGTCGGGTATCGACC
ACCTGGATATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V5MS03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.327

95.503

0.595
  pilU Acinetobacter baylyi ADP1

59.384

94.444

0.561
  pilU Vibrio cholerae strain A1552

54.286

92.593

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

42.136

89.153

0.376
  pilT Legionella pneumophila strain Lp02

43.925

84.921

0.373
  pilT Legionella pneumophila strain ERS1305867

43.925

84.921

0.373
  pilT Acinetobacter baylyi ADP1

43.614

84.921

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.153

0.37
  pilT Acinetobacter nosocomialis M2

42.042

88.095

0.37
  pilT Acinetobacter baumannii D1279779

42.042

88.095

0.37
  pilT Acinetobacter baumannii strain A118

42.042

88.095

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368

Multiple sequence alignment