Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFU65_RS06585 Genome accession   NZ_CP155964
Coordinates   1564896..1565933 (+) Length   345 a.a.
NCBI ID   WP_407472198.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BR25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1559896..1570933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU65_RS06555 (ABFU65_06540) - 1560331..1560870 (-) 540 WP_323475273.1 hypothetical protein -
  ABFU65_RS06560 (ABFU65_06545) - 1560924..1561232 (-) 309 WP_014508439.1 DUF3861 domain-containing protein -
  ABFU65_RS06565 (ABFU65_06550) - 1561325..1561915 (-) 591 WP_323531009.1 M23 family metallopeptidase -
  ABFU65_RS06570 (ABFU65_06555) - 1562059..1562481 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU65_RS06575 (ABFU65_06560) proC 1563025..1563885 (-) 861 WP_274341798.1 pyrroline-5-carboxylate reductase -
  ABFU65_RS06580 (ABFU65_06565) - 1563926..1564609 (-) 684 WP_274341799.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU65_RS06585 (ABFU65_06570) pilT 1564896..1565933 (+) 1038 WP_407472198.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU65_RS06590 (ABFU65_06575) pilU 1566056..1567186 (+) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU65_RS06595 (ABFU65_06580) - 1567466..1568140 (+) 675 WP_014508435.1 YitT family protein -
  ABFU65_RS06600 (ABFU65_06585) - 1568154..1569053 (-) 900 WP_323550377.1 DUF72 domain-containing protein -
  ABFU65_RS06605 (ABFU65_06590) - 1569276..1570538 (-) 1263 WP_323550376.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38669.33 Da        Isoelectric Point: 6.5930

>NTDB_id=1000375 ABFU65_RS06585 WP_407472198.1 1564896..1565933(+) (pilT) [Xanthomonas campestris pv. raphani strain BR25]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLYTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1000375 ABFU65_RS06585 WP_407472198.1 1564896..1565933(+) (pilT) [Xanthomonas campestris pv. raphani strain BR25]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGTTGGACCATAAGCAGGTGCACGCGCTGGTTTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAATCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGTTGATCGACCAGCCGCAGGGTCTGATCCTGGTGACCGGCCCGACCGGGT
CGGGCAAGTCGACCACGCTTGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGGCACCTTGTACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCGCAGGC
ATTGCTCAAGAAAGTGGGCGGCGGGCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment