Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU65_RS06590 Genome accession   NZ_CP155964
Coordinates   1566056..1567186 (+) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas campestris pv. raphani strain BR25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1561056..1572186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU65_RS06565 (ABFU65_06550) - 1561325..1561915 (-) 591 WP_323531009.1 M23 family metallopeptidase -
  ABFU65_RS06570 (ABFU65_06555) - 1562059..1562481 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU65_RS06575 (ABFU65_06560) proC 1563025..1563885 (-) 861 WP_274341798.1 pyrroline-5-carboxylate reductase -
  ABFU65_RS06580 (ABFU65_06565) - 1563926..1564609 (-) 684 WP_274341799.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU65_RS06585 (ABFU65_06570) pilT 1564896..1565933 (+) 1038 WP_407472198.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU65_RS06590 (ABFU65_06575) pilU 1566056..1567186 (+) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU65_RS06595 (ABFU65_06580) - 1567466..1568140 (+) 675 WP_014508435.1 YitT family protein -
  ABFU65_RS06600 (ABFU65_06585) - 1568154..1569053 (-) 900 WP_323550377.1 DUF72 domain-containing protein -
  ABFU65_RS06605 (ABFU65_06590) - 1569276..1570538 (-) 1263 WP_323550376.1 hypothetical protein -
  ABFU65_RS06610 (ABFU65_06595) - 1570535..1571074 (-) 540 WP_323550375.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=1000376 ABFU65_RS06590 WP_005990431.1 1566056..1567186(+) (pilU) [Xanthomonas campestris pv. raphani strain BR25]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1000376 ABFU65_RS06590 WP_005990431.1 1566056..1567186(+) (pilU) [Xanthomonas campestris pv. raphani strain BR25]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATTTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTGGGCATGGTGCTGCGTCGGATCGAGACGCGCATTCC
CACCGTGGAGGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGTATCGATACCGATAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGTACCCGCGAAGGCA
TGGACCATGCTATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGAAGACGCACTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCCAAGACGCTGTCGCAGGGGCTCGATGGCGTGGAGATTGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment