Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 1873473..1874062 Replicon chromosome
Accession NC_013971
Organism Erwinia amylovora ATCC 49946

Toxin (Protein)


Gene name doc Uniprot ID D4I2L7
Locus tag EAM_RS08440 Protein ID WP_004157545.1
Coordinates 1873691..1874062 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID D4I2L6
Locus tag EAM_RS08435 Protein ID WP_004157544.1
Coordinates 1873473..1873694 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAM_RS18300 (EAM_1733) 1869450..1869659 + 210 WP_004157539.1 glycine zipper 2TM domain-containing protein -
EAM_RS08420 (EAM_1734) 1869772..1870248 + 477 WP_004157540.1 GNAT family N-acetyltransferase -
EAM_RS08425 (EAM_1735) 1870372..1871121 + 750 WP_004157541.1 5'-nucleotidase, lipoprotein e(P4) family -
EAM_RS08430 (EAM_1736) 1871208..1872680 - 1473 WP_004157542.1 catalase -
EAM_RS08435 (EAM_1737) 1873473..1873694 + 222 WP_004157544.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EAM_RS08440 (EAM_1738) 1873691..1874062 + 372 WP_004157545.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EAM_RS08445 (EAM_1741) 1874946..1876886 + 1941 WP_004157559.1 methyl-accepting chemotaxis protein -
EAM_RS08450 (EAM_1742) 1877012..1877647 + 636 WP_004157562.1 carbonic anhydrase -
EAM_RS08455 (EAM_1743) 1877732..1878181 - 450 WP_004157563.1 DUF4385 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1858737..1876886 18149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(26-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13667.70 Da        Isoelectric Point: 7.4182

>T24688 WP_004157545.1 NC_013971:1873691-1874062 [Erwinia amylovora ATCC 49946]
MIYFLSSQDIIGIHQRMIVAYGGLAGYADPGRIESMATRILNRHIYEGEDDIYILAAAYLLAIARGHCFNDANKRTAFAS
TALFLRRNGILLRFSPIHEQLTVSAAQGSLDVWHIAEALKQST

Download         Length: 372 bp

>T24688 NC_013971:1873691-1874062 [Erwinia amylovora ATCC 49946]
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8173.24 Da        Isoelectric Point: 4.9861

>AT24688 WP_004157544.1 NC_013971:1873473-1873694 [Erwinia amylovora ATCC 49946]
MQAINFTTARNELASVLDSVASGEPVMITRRSAKSVVVVDAAQYEKMQKIQAESDFDWLFTEHGQTLEALKNR

Download         Length: 222 bp

>AT24688 NC_013971:1873473-1873694 [Erwinia amylovora ATCC 49946]
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4I2L7

Antitoxin

Source ID Structure
AlphaFold DB A0A831A2K1

References