206030

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3679800..3680389 Replicon chromosome
Accession NZ_CP076596
Organism Erwinia amylovora isolate CP20161301

Toxin (Protein)


Gene name doc Uniprot ID D4I2L7
Locus tag KQH32_RS16735 Protein ID WP_004157545.1
Coordinates 3680018..3680389 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID D4I2L6
Locus tag KQH32_RS16730 Protein ID WP_004157544.1
Coordinates 3679800..3680021 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH32_RS16710 (KQH32_16715) 3675777..3675986 + 210 WP_004157539.1 glycine zipper 2TM domain-containing protein -
KQH32_RS16715 (KQH32_16720) 3676099..3676575 + 477 WP_004157540.1 GNAT family N-acetyltransferase -
KQH32_RS16720 (KQH32_16725) 3676699..3677448 + 750 WP_004157541.1 5'-nucleotidase, lipoprotein e(P4) family -
KQH32_RS16725 (KQH32_16730) 3677535..3679007 - 1473 WP_004157542.1 catalase -
KQH32_RS16730 (KQH32_16735) 3679800..3680021 + 222 WP_004157544.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KQH32_RS16735 (KQH32_16740) 3680018..3680389 + 372 WP_004157545.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KQH32_RS16740 (KQH32_16745) 3681273..3683213 + 1941 WP_004157559.1 methyl-accepting chemotaxis protein -
KQH32_RS16745 (KQH32_16750) 3683339..3683974 + 636 WP_004157562.1 carbonic anhydrase -
KQH32_RS16750 (KQH32_16755) 3684059..3684508 - 450 WP_004157563.1 DUF4385 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3665064..3683213 18149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(26-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13667.70 Da        Isoelectric Point: 7.4182

>T206030 WP_004157545.1 NZ_CP076596:3680018-3680389 [Erwinia amylovora]
MIYFLSSQDIIGIHQRMIVAYGGLAGYADPGRIESMATRILNRHIYEGEDDIYILAAAYLLAIARGHCFNDANKRTAFAS
TALFLRRNGILLRFSPIHEQLTVSAAQGSLDVWHIAEALKQST

Download         Length: 372 bp

>T206030 NZ_CP102077:358432-358779 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8173.24 Da        Isoelectric Point: 4.9861

>AT206030 WP_004157544.1 NZ_CP076596:3679800-3680021 [Erwinia amylovora]
MQAINFTTARNELASVLDSVASGEPVMITRRSAKSVVVVDAAQYEKMQKIQAESDFDWLFTEHGQTLEALKNR

Download         Length: 222 bp

>AT206030 NZ_CP102077:358779-359078 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4I2L7

Antitoxin

Source ID Structure
AlphaFold DB A0A831A2K1

References