Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3694695..3695284 Replicon chromosome
Accession NZ_CP076592
Organism Erwinia amylovora isolate CP20140001

Toxin (Protein)


Gene name doc Uniprot ID D4I2L7
Locus tag KQH33_RS16795 Protein ID WP_004157545.1
Coordinates 3694913..3695284 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID D4I2L6
Locus tag KQH33_RS16790 Protein ID WP_004157544.1
Coordinates 3694695..3694916 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH33_RS16770 (KQH33_16775) 3690672..3690881 + 210 WP_004157539.1 glycine zipper 2TM domain-containing protein -
KQH33_RS16775 (KQH33_16780) 3690994..3691470 + 477 WP_004157540.1 GNAT family N-acetyltransferase -
KQH33_RS16780 (KQH33_16785) 3691594..3692343 + 750 WP_004157541.1 5'-nucleotidase, lipoprotein e(P4) family -
KQH33_RS16785 (KQH33_16790) 3692430..3693902 - 1473 WP_004157542.1 catalase -
KQH33_RS16790 (KQH33_16795) 3694695..3694916 + 222 WP_004157544.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KQH33_RS16795 (KQH33_16800) 3694913..3695284 + 372 WP_004157545.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KQH33_RS16800 (KQH33_16805) 3696168..3698108 + 1941 WP_004157559.1 methyl-accepting chemotaxis protein -
KQH33_RS16805 (KQH33_16810) 3698234..3698869 + 636 WP_004157562.1 carbonic anhydrase -
KQH33_RS16810 (KQH33_16815) 3698954..3699403 - 450 WP_004157563.1 DUF4385 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3685087..3698108 13021


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(26-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13667.70 Da        Isoelectric Point: 7.4182

>T206000 WP_004157545.1 NZ_CP076592:3694913-3695284 [Erwinia amylovora]
MIYFLSSQDIIGIHQRMIVAYGGLAGYADPGRIESMATRILNRHIYEGEDDIYILAAAYLLAIARGHCFNDANKRTAFAS
TALFLRRNGILLRFSPIHEQLTVSAAQGSLDVWHIAEALKQST

Download         Length: 372 bp

>T206000 NZ_CP102061:3458859-3459209 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8173.24 Da        Isoelectric Point: 4.9861

>AT206000 WP_004157544.1 NZ_CP076592:3694695-3694916 [Erwinia amylovora]
MQAINFTTARNELASVLDSVASGEPVMITRRSAKSVVVVDAAQYEKMQKIQAESDFDWLFTEHGQTLEALKNR

Download         Length: 222 bp

>AT206000 NZ_CP102061:3458614-3458865 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4I2L7

Antitoxin

Source ID Structure
AlphaFold DB A0A831A2K1

References