Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 1750402..1750991 Replicon chromosome
Accession NZ_CP076594
Organism Erwinia amylovora isolate CP20086202

Toxin (Protein)


Gene name doc Uniprot ID D4I2L7
Locus tag KQH36_RS07775 Protein ID WP_004157545.1
Coordinates 1750402..1750773 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID D4I2L6
Locus tag KQH36_RS07780 Protein ID WP_004157544.1
Coordinates 1750770..1750991 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH36_RS07760 (KQH36_07780) 1746283..1746732 + 450 WP_004157563.1 DUF4385 domain-containing protein -
KQH36_RS07765 (KQH36_07785) 1746817..1747452 - 636 WP_004157562.1 carbonic anhydrase -
KQH36_RS07770 (KQH36_07790) 1747578..1749518 - 1941 WP_004157559.1 methyl-accepting chemotaxis protein -
KQH36_RS07775 (KQH36_07795) 1750402..1750773 - 372 WP_004157545.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KQH36_RS07780 (KQH36_07800) 1750770..1750991 - 222 WP_004157544.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KQH36_RS07785 (KQH36_07805) 1751784..1753256 + 1473 WP_004157542.1 catalase -
KQH36_RS07790 (KQH36_07810) 1753343..1754092 - 750 WP_004157541.1 5'-nucleotidase, lipoprotein e(P4) family -
KQH36_RS07795 (KQH36_07815) 1754216..1754692 - 477 WP_004157540.1 GNAT family N-acetyltransferase -
KQH36_RS07800 (KQH36_07820) 1754805..1755014 - 210 WP_004157539.1 glycine zipper 2TM domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1750402..1766663 16261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(26-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13667.70 Da        Isoelectric Point: 7.4182

>T206007 WP_004157545.1 NZ_CP076594:c1750773-1750402 [Erwinia amylovora]
MIYFLSSQDIIGIHQRMIVAYGGLAGYADPGRIESMATRILNRHIYEGEDDIYILAAAYLLAIARGHCFNDANKRTAFAS
TALFLRRNGILLRFSPIHEQLTVSAAQGSLDVWHIAEALKQST

Download         Length: 372 bp

>T206007 NZ_CP102061:c5065048-5064941 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8173.24 Da        Isoelectric Point: 4.9861

>AT206007 WP_004157544.1 NZ_CP076594:c1750991-1750770 [Erwinia amylovora]
MQAINFTTARNELASVLDSVASGEPVMITRRSAKSVVVVDAAQYEKMQKIQAESDFDWLFTEHGQTLEALKNR

Download         Length: 222 bp

>AT206007 NZ_CP102061:5065096-5065162 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4I2L7

Antitoxin

Source ID Structure
AlphaFold DB A0A831A2K1

References