Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 1872744..1873333 Replicon chromosome
Accession NZ_CP024970
Organism Erwinia amylovora strain E-2

Toxin (Protein)


Gene name doc Uniprot ID D4I2L7
Locus tag AD997_RS08870 Protein ID WP_004157545.1
Coordinates 1872962..1873333 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID D4I2L6
Locus tag AD997_RS08865 Protein ID WP_004157544.1
Coordinates 1872744..1872965 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD997_RS08840 1868721..1868930 + 210 WP_004157539.1 glycine zipper 2TM domain-containing protein -
AD997_RS08845 1869043..1869519 + 477 WP_004157540.1 GNAT family N-acetyltransferase -
AD997_RS08850 1869643..1870392 + 750 WP_004157541.1 5'-nucleotidase, lipoprotein e(P4) family -
AD997_RS08855 1870479..1871951 - 1473 WP_004157542.1 catalase -
AD997_RS08865 1872744..1872965 + 222 WP_004157544.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AD997_RS08870 1872962..1873333 + 372 WP_004157545.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
AD997_RS08875 1874217..1876157 + 1941 WP_004157559.1 HAMP domain-containing protein -
AD997_RS08880 1876283..1876918 + 636 WP_004157562.1 carbonic anhydrase -
AD997_RS08885 1877003..1877452 - 450 WP_004157563.1 DUF4385 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1863136..1876157 13021


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(26-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13667.70 Da        Isoelectric Point: 7.4182

>T89360 WP_004157545.1 NZ_CP024970:1872962-1873333 [Erwinia amylovora]
MIYFLSSQDIIGIHQRMIVAYGGLAGYADPGRIESMATRILNRHIYEGEDDIYILAAAYLLAIARGHCFNDANKRTAFAS
TALFLRRNGILLRFSPIHEQLTVSAAQGSLDVWHIAEALKQST

Download         Length: 372 bp

>T89360 NZ_CP024970:1872962-1873333 [Erwinia amylovora]
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8173.24 Da        Isoelectric Point: 4.9861

>AT89360 WP_004157544.1 NZ_CP024970:1872744-1872965 [Erwinia amylovora]
MQAINFTTARNELASVLDSVASGEPVMITRRSAKSVVVVDAAQYEKMQKIQAESDFDWLFTEHGQTLEALKNR

Download         Length: 222 bp

>AT89360 NZ_CP024970:1872744-1872965 [Erwinia amylovora]
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4I2L7

Antitoxin

Source ID Structure
AlphaFold DB A0A831A2K1

References