Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 252991..253580 Replicon chromosome
Accession NZ_CP076595
Organism Erwinia amylovora isolate CP20130202

Toxin (Protein)


Gene name doc Uniprot ID D4I2L7
Locus tag KQH34_RS01150 Protein ID WP_004157545.1
Coordinates 252991..253362 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID D4I2L6
Locus tag KQH34_RS01155 Protein ID WP_004157544.1
Coordinates 253359..253580 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH34_RS01135 (KQH34_01145) 248872..249321 + 450 WP_004157563.1 DUF4385 domain-containing protein -
KQH34_RS01140 (KQH34_01150) 249406..250041 - 636 WP_004157562.1 carbonic anhydrase -
KQH34_RS01145 (KQH34_01155) 250167..252107 - 1941 WP_004157559.1 methyl-accepting chemotaxis protein -
KQH34_RS01150 (KQH34_01160) 252991..253362 - 372 WP_004157545.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KQH34_RS01155 (KQH34_01165) 253359..253580 - 222 WP_004157544.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KQH34_RS01160 (KQH34_01170) 254373..255845 + 1473 WP_004157542.1 catalase -
KQH34_RS01165 (KQH34_01175) 255932..256681 - 750 WP_004157541.1 5'-nucleotidase, lipoprotein e(P4) family -
KQH34_RS01170 (KQH34_01180) 256805..257281 - 477 WP_004157540.1 GNAT family N-acetyltransferase -
KQH34_RS01175 (KQH34_01185) 257394..257603 - 210 WP_004157539.1 glycine zipper 2TM domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 252991..269252 16261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(26-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13667.70 Da        Isoelectric Point: 7.4182

>T206013 WP_004157545.1 NZ_CP076595:c253362-252991 [Erwinia amylovora]
MIYFLSSQDIIGIHQRMIVAYGGLAGYADPGRIESMATRILNRHIYEGEDDIYILAAAYLLAIARGHCFNDANKRTAFAS
TALFLRRNGILLRFSPIHEQLTVSAAQGSLDVWHIAEALKQST

Download         Length: 372 bp

>T206013 NZ_CP102065:c2692662-2692321 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
TTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8173.24 Da        Isoelectric Point: 4.9861

>AT206013 WP_004157544.1 NZ_CP076595:c253580-253359 [Erwinia amylovora]
MQAINFTTARNELASVLDSVASGEPVMITRRSAKSVVVVDAAQYEKMQKIQAESDFDWLFTEHGQTLEALKNR

Download         Length: 222 bp

>AT206013 NZ_CP102065:c2692892-2692662 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4I2L7

Antitoxin

Source ID Structure
AlphaFold DB A0A831A2K1

References