Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 1932751..1933340 Replicon chromosome
Accession NZ_CP050240
Organism Erwinia amylovora strain FB-20

Toxin (Protein)


Gene name doc Uniprot ID D4I2L7
Locus tag EGZ89_RS08550 Protein ID WP_004157545.1
Coordinates 1932751..1933122 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID D4I2L6
Locus tag EGZ89_RS08555 Protein ID WP_004157544.1
Coordinates 1933119..1933340 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGZ89_RS08535 1928632..1929081 + 450 WP_004157563.1 DUF4385 domain-containing protein -
EGZ89_RS08540 1929166..1929801 - 636 WP_004157562.1 carbonic anhydrase -
EGZ89_RS08545 1929927..1931867 - 1941 WP_004157559.1 HAMP domain-containing protein -
EGZ89_RS08550 1932751..1933122 - 372 WP_004157545.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EGZ89_RS08555 1933119..1933340 - 222 WP_004157544.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EGZ89_RS08560 1934133..1935605 + 1473 WP_004157542.1 catalase -
EGZ89_RS08565 1935692..1936441 - 750 WP_004157541.1 5'-nucleotidase, lipoprotein e(P4) family -
EGZ89_RS08570 1936565..1937041 - 477 WP_004157540.1 GNAT family N-acetyltransferase -
EGZ89_RS08575 1937154..1937363 - 210 WP_004157539.1 glycine zipper 2TM domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1932751..1949012 16261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(26-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13667.70 Da        Isoelectric Point: 7.4182

>T152395 WP_004157545.1 NZ_CP050240:c1933122-1932751 [Erwinia amylovora]
MIYFLSSQDIIGIHQRMIVAYGGLAGYADPGRIESMATRILNRHIYEGEDDIYILAAAYLLAIARGHCFNDANKRTAFAS
TALFLRRNGILLRFSPIHEQLTVSAAQGSLDVWHIAEALKQST

Download         Length: 372 bp

>T152395 NZ_CP066143:5128600-5129136 [Klebsiella pneumoniae U-0608239]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGT
TTGTTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAAC
TTACTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8173.24 Da        Isoelectric Point: 4.9861

>AT152395 WP_004157544.1 NZ_CP050240:c1933340-1933119 [Erwinia amylovora]
MQAINFTTARNELASVLDSVASGEPVMITRRSAKSVVVVDAAQYEKMQKIQAESDFDWLFTEHGQTLEALKNR

Download         Length: 222 bp

>AT152395 NZ_CP066143:5128323-5128589 [Klebsiella pneumoniae U-0608239]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
GGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D4I2L7

Antitoxin

Source ID Structure
AlphaFold DB A0A831A2K1

References