Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU26_RS03490 Genome accession   NZ_CP155935
Coordinates   779705..780838 (-) Length   377 a.a.
NCBI ID   WP_115000054.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain Xcr-TH1B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 774705..785838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU26_RS03460 (ABFU26_03460) - 774727..775062 (-) 336 WP_011035833.1 phasin family protein -
  ABFU26_RS03465 (ABFU26_03465) queD 775252..775605 (-) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ABFU26_RS03470 (ABFU26_03470) - 775721..777109 (-) 1389 WP_011035834.1 DEAD/DEAH box helicase -
  ABFU26_RS03475 (ABFU26_03475) - 777489..778478 (+) 990 WP_323546232.1 fumarylacetoacetate hydrolase family protein -
  ABFU26_RS03480 (ABFU26_03480) maiA 778522..779184 (+) 663 WP_227971066.1 maleylacetoacetate isomerase -
  ABFU26_RS03485 (ABFU26_03485) - 779353..779597 (+) 245 Protein_676 hypothetical protein -
  ABFU26_RS03490 (ABFU26_03490) pilU 779705..780838 (-) 1134 WP_115000054.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU26_RS03495 (ABFU26_03495) - 780937..781320 (+) 384 WP_011035838.1 DUF4398 domain-containing protein -
  ABFU26_RS03500 (ABFU26_03500) - 781317..782207 (+) 891 WP_116902720.1 hypothetical protein -
  ABFU26_RS03505 (ABFU26_03505) - 782549..782767 (+) 219 WP_019237985.1 YdcH family protein -
  ABFU26_RS03510 (ABFU26_03510) - 782999..784369 (+) 1371 WP_014506550.1 pyridoxal-phosphate dependent enzyme -
  ABFU26_RS03515 (ABFU26_03515) - 784463..785662 (+) 1200 WP_407460821.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41809.14 Da        Isoelectric Point: 6.1401

>NTDB_id=999894 ABFU26_RS03490 WP_115000054.1 779705..780838(-) (pilU) [Xanthomonas campestris pv. raphani strain Xcr-TH1B]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=999894 ABFU26_RS03490 WP_115000054.1 779705..780838(-) (pilU) [Xanthomonas campestris pv. raphani strain Xcr-TH1B]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCAATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTAGGCCTGGACACGCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCATAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGCCTGGACGGGCGCCGCCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTAC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCTGCCGACTCGCGCGACGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGGGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361
  pilT Neisseria gonorrhoeae MS11

39.193

92.042

0.361
  pilT Neisseria meningitidis 8013

39.193

92.042

0.361

Multiple sequence alignment