Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFU07_RS06980 Genome accession   NZ_CP155923
Coordinates   1658754..1659791 (+) Length   345 a.a.
NCBI ID   WP_407466430.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain G3-27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653754..1664791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU07_RS06950 (ABFU07_06930) - 1654192..1654731 (-) 540 WP_323527060.1 hypothetical protein -
  ABFU07_RS06955 (ABFU07_06935) - 1654785..1655093 (-) 309 WP_014508439.1 DUF3861 domain-containing protein -
  ABFU07_RS06960 (ABFU07_06940) - 1655186..1655776 (-) 591 WP_228944683.1 M23 family metallopeptidase -
  ABFU07_RS06965 (ABFU07_06945) - 1655920..1656342 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU07_RS06970 (ABFU07_06950) proC 1656883..1657743 (-) 861 WP_228944684.1 pyrroline-5-carboxylate reductase -
  ABFU07_RS06975 (ABFU07_06955) - 1657784..1658467 (-) 684 WP_228944685.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU07_RS06980 (ABFU07_06960) pilT 1658754..1659791 (+) 1038 WP_407466430.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU07_RS06985 (ABFU07_06965) pilU 1659914..1661044 (+) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU07_RS06990 (ABFU07_06970) - 1661324..1661998 (+) 675 WP_228944705.1 YitT family protein -
  ABFU07_RS06995 (ABFU07_06975) - 1662012..1662911 (-) 900 WP_407298497.1 DUF72 domain-containing protein -
  ABFU07_RS07000 (ABFU07_06980) - 1663134..1664396 (-) 1263 WP_228944687.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38671.35 Da        Isoelectric Point: 6.8357

>NTDB_id=999684 ABFU07_RS06980 WP_407466430.1 1658754..1659791(+) (pilT) [Xanthomonas campestris pv. raphani strain G3-27]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINRREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=999684 ABFU07_RS06980 WP_407466430.1 1658754..1659791(+) (pilT) [Xanthomonas campestris pv. raphani strain G3-27]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCCTTGGACCATAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGT
TTTCGCGTCAATGCGTTCAATCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGTTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGTTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGGT
CGGGCAAGTCGACCACGCTTGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTTGTGCACACCTCGCAGAAGTGCCTGATCAACCGGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCGCAGGC
ATTGCTCAAGAAAGTGGGCGGCGGACGTACCGCAGCGTGGGAGATCATGGTCGGCACTCCGGCTATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGATCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment