Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMN23_RS00905 Genome accession   NZ_AP027151
Coordinates   198429..199490 (+) Length   353 a.a.
NCBI ID   WP_282001226.1    Uniprot ID   -
Organism   Geotalea uraniireducens strain NIT-SL11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 193429..204490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMN23_RS00885 (GURASL_01690) pdxA 193488..194507 (+) 1020 WP_282001211.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
  QMN23_RS00890 (GURASL_01700) - 194574..195548 (+) 975 WP_282001212.1 biosynthetic peptidoglycan transglycosylase -
  QMN23_RS00895 (GURASL_01710) - 195590..196594 (-) 1005 WP_282001214.1 hypothetical protein -
  QMN23_RS00900 (GURASL_01720) - 196767..198416 (+) 1650 WP_282001225.1 ATPase, T2SS/T4P/T4SS family -
  QMN23_RS00905 (GURASL_01730) pilT 198429..199490 (+) 1062 WP_282001226.1 type IV pilus twitching motility protein PilT Machinery gene
  QMN23_RS00915 (GURASL_01740) - 199768..201189 (+) 1422 WP_282001227.1 UbiD family decarboxylase -
  QMN23_RS00920 (GURASL_01750) - 201186..201710 (+) 525 WP_282001228.1 hypothetical protein -
  QMN23_RS00925 (GURASL_01760) - 201724..202593 (+) 870 WP_282001229.1 4-hydroxybenzoate octaprenyltransferase -
  QMN23_RS00930 (GURASL_01770) - 202593..203210 (+) 618 WP_282003781.1 flavin prenyltransferase UbiX -
  QMN23_RS00935 (GURASL_01780) mqnE 203207..204289 (+) 1083 WP_282001230.1 aminofutalosine synthase MqnE -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39348.52 Da        Isoelectric Point: 7.0746

>NTDB_id=99729 QMN23_RS00905 WP_282001226.1 198429..199490(+) (pilT) [Geotalea uraniireducens strain NIT-SL11]
MARIDALFKMMKEQGASDLHLSTGAPPIFRLHGEMVRQNFKALTHDELRGILFEILSAKQQEEFDARHDLDFAYAIPGLA
RFRGNYMMQHRGIAAVFRIIPSKILSADDLSLPEGVRKMTQFKKGLVLVTGPTGSGKSTTLAAMIDLINSTRQEHILTLE
DPLEFIHENKLSLLNQRQIGEHSDSFTNALRAALREDPDVILVGEMRDLETISLAMSAAETGHLVFGTLHTNSAAKTIDR
IIDVFPKDGQEQVRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIANLIREGKTFQIPSIIQTAKRDGMQLMDQ
HLLDLLKMKRVAAEEAYRCAQDKKQFEQYLDKA

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=99729 QMN23_RS00905 WP_282001226.1 198429..199490(+) (pilT) [Geotalea uraniireducens strain NIT-SL11]
ATGGCACGGATTGATGCACTTTTCAAGATGATGAAGGAACAGGGGGCGTCCGACCTACATCTGTCGACCGGGGCGCCGCC
AATTTTCCGGCTCCATGGCGAGATGGTCCGGCAGAATTTCAAGGCGCTGACCCATGATGAGCTGAGGGGGATTCTGTTCG
AAATCCTGTCGGCCAAGCAGCAGGAGGAATTCGACGCGAGGCACGACCTCGACTTCGCTTACGCAATTCCGGGGCTGGCC
CGCTTCCGGGGGAATTATATGATGCAGCACCGGGGGATCGCCGCCGTCTTCCGGATTATCCCGAGCAAAATCCTGTCGGC
CGATGATCTGAGCCTGCCTGAAGGGGTACGGAAGATGACCCAGTTCAAAAAAGGGCTGGTGCTGGTGACGGGGCCGACCG
GTTCGGGGAAATCGACGACCTTGGCGGCCATGATTGATCTGATCAATTCCACCCGCCAGGAGCATATCCTCACCCTGGAG
GACCCGCTCGAATTCATTCATGAAAACAAACTTTCGCTGCTTAATCAGCGGCAGATCGGCGAGCACTCGGACAGCTTTAC
CAACGCGCTGCGGGCGGCGTTGCGCGAAGACCCCGACGTTATCCTGGTTGGGGAGATGCGGGACCTGGAGACGATTAGCC
TCGCCATGAGCGCTGCCGAAACGGGCCACTTGGTCTTCGGCACGCTGCATACCAATTCGGCGGCCAAGACCATTGACCGA
ATTATCGATGTCTTTCCGAAAGATGGCCAGGAACAGGTGCGGGCCATGCTTTCCGAATCGCTCAAGGGGGTAGTGTGCCA
GCAATTATTGAAGACCGCCGACGGGAAAGGGCGGGTGGCTGCCCTGGAAATCATGCTCGGCACGCCGGCCATCGCCAACC
TGATCCGGGAGGGGAAAACCTTTCAGATTCCGTCGATCATCCAGACCGCCAAGCGGGACGGCATGCAGCTGATGGATCAG
CACCTGCTCGATCTGTTAAAGATGAAGCGGGTTGCTGCGGAGGAGGCGTACCGCTGTGCCCAGGACAAGAAACAGTTCGA
GCAGTACCTGGATAAAGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

57.402

93.768

0.538
  pilT Legionella pneumophila strain ERS1305867

57.402

93.768

0.538
  pilT Pseudomonas stutzeri DSM 10701

55.102

97.167

0.535
  pilT Acinetobacter baylyi ADP1

54.519

97.167

0.53
  pilT Neisseria meningitidis 8013

53.801

96.884

0.521
  pilT Acinetobacter nosocomialis M2

53.644

97.167

0.521
  pilT Acinetobacter baumannii D1279779

53.644

97.167

0.521
  pilT Acinetobacter baumannii strain A118

53.644

97.167

0.521
  pilT Pseudomonas aeruginosa PAK

53.644

97.167

0.521
  pilT Neisseria gonorrhoeae MS11

53.509

96.884

0.518
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.12

94.051

0.518
  pilT Vibrio cholerae strain A1552

55.12

94.051

0.518
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.714

99.15

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.361

94.051

0.38
  pilU Vibrio cholerae strain A1552

40.615

92.068

0.374
  pilU Acinetobacter baylyi ADP1

39.104

94.901

0.371

Multiple sequence alignment