Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QMG27_RS03120 Genome accession   NZ_AP027058
Coordinates   625823..626845 (+) Length   340 a.a.
NCBI ID   WP_281813097.1    Uniprot ID   -
Organism   Limnohabitans sp. MORI2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 620823..631845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG27_RS03095 (LMORI2_06230) - 621144..622310 (+) 1167 WP_281813087.1 MFS transporter -
  QMG27_RS03100 (LMORI2_06240) - 622340..622552 (-) 213 WP_281813088.1 DUF2892 domain-containing protein -
  QMG27_RS03105 (LMORI2_06250) - 622674..624884 (+) 2211 WP_281813091.1 SLC26A/SulP transporter family protein -
  QMG27_RS03110 (LMORI2_06260) - 624891..625274 (-) 384 WP_281813093.1 VOC family protein -
  QMG27_RS03115 (LMORI2_06270) - 625353..625637 (-) 285 WP_281813095.1 HU family DNA-binding protein -
  QMG27_RS03120 (LMORI2_06280) pilT 625823..626845 (+) 1023 WP_281813097.1 type IV pilus twitching motility protein PilT Machinery gene
  QMG27_RS03130 (LMORI2_06290) - 626945..628129 (-) 1185 WP_281813099.1 VWA domain-containing protein -
  QMG27_RS03135 (LMORI2_06300) - 628138..628353 (-) 216 WP_281813101.1 hypothetical protein -
  QMG27_RS03140 (LMORI2_06310) - 628367..629221 (-) 855 WP_281813104.1 MoxR family ATPase -
  QMG27_RS03145 (LMORI2_06320) - 629268..630437 (-) 1170 WP_281813106.1 CoA transferase -
  QMG27_RS03150 (LMORI2_06330) - 630441..631433 (-) 993 WP_281813108.1 tripartite tricarboxylate transporter substrate binding protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37027.66 Da        Isoelectric Point: 6.4973

>NTDB_id=99440 QMG27_RS03120 WP_281813097.1 625823..626845(+) (pilT) [Limnohabitans sp. MORI2]
MTLSNLLLQCITRGASDLHLSSGLAPLLRVQGDLVALNEPALVSEELHASLMTMMSSSQQTQFAQTHECDFSWDLPELSR
FRVNVFMQARGISAVFRVIPHHVPSLQDLDAPLVLSQLMNRTQGLVLVTGATGSGKSTTLAAMIHMLNQTQDKHIITIED
PIEFVHVPARCLIQQRELGPHTLSFANALRAALREDPDVILVGELRDLETIRLAITAAETGHLVFATLHTSSAAKTMDRI
VDVFPAQEKDMVRSMLSESLHAVVSQTLCKRKDGAGRVAAFEVMLGTPAVRNLVREGKVAQLYSTIQTGRSVGMQTLDQS
LAGLVQQGLIDAQEAQAQSK

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=99440 QMG27_RS03120 WP_281813097.1 625823..626845(+) (pilT) [Limnohabitans sp. MORI2]
ATGACGCTTTCTAATTTGTTGTTGCAATGCATCACACGCGGTGCATCCGACCTGCATTTGTCCTCTGGCCTTGCGCCTTT
GCTGCGTGTGCAAGGTGATTTGGTGGCCTTGAATGAACCCGCGTTGGTCAGCGAAGAGTTGCATGCGTCATTGATGACGA
TGATGTCATCCAGCCAGCAAACACAATTTGCGCAAACCCATGAATGTGATTTTTCATGGGACCTGCCAGAGCTGTCACGC
TTTCGGGTGAATGTGTTCATGCAGGCACGGGGTATCTCTGCCGTGTTTCGGGTGATTCCACATCATGTGCCTAGTCTGCA
AGATCTTGATGCCCCGCTTGTGCTGAGTCAGTTGATGAATCGCACACAGGGTTTGGTGTTGGTCACAGGTGCCACCGGTT
CTGGCAAGTCCACCACCTTGGCAGCGATGATTCACATGCTCAATCAAACGCAAGACAAACACATCATCACCATTGAAGAT
CCGATCGAGTTTGTGCATGTGCCAGCACGTTGCTTGATTCAACAACGTGAACTCGGCCCCCATACCTTGAGCTTTGCCAA
CGCGCTGCGTGCAGCCTTGCGCGAAGACCCCGATGTGATTTTGGTCGGCGAGCTGCGCGACTTAGAAACCATTCGCCTAG
CCATCACTGCGGCTGAAACAGGGCACTTGGTGTTCGCAACCTTGCACACCTCGAGTGCGGCCAAAACCATGGACCGCATC
GTGGATGTGTTTCCTGCGCAAGAAAAAGACATGGTGCGCAGCATGTTGTCTGAGTCACTCCATGCGGTGGTGTCGCAGAC
CTTGTGCAAGCGCAAAGACGGTGCAGGGCGTGTAGCCGCATTTGAGGTGATGTTGGGGACGCCTGCCGTGCGCAACTTGG
TGCGAGAGGGTAAAGTGGCGCAACTGTATTCAACCATCCAAACAGGGCGCAGTGTGGGGATGCAAACCTTAGATCAGAGC
TTGGCTGGCTTGGTACAGCAGGGCTTGATTGATGCGCAAGAGGCGCAAGCCCAATCGAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

60.588

100

0.606
  pilT Pseudomonas aeruginosa PAK

60

100

0.6
  pilT Neisseria meningitidis 8013

59.118

100

0.591
  pilT Vibrio cholerae strain A1552

59.467

99.412

0.591
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

59.467

99.412

0.591
  pilT Neisseria gonorrhoeae MS11

58.824

100

0.588
  pilT Acinetobacter baylyi ADP1

58.824

100

0.588
  pilT Acinetobacter baumannii strain A118

57.647

100

0.576
  pilT Acinetobacter nosocomialis M2

57.647

100

0.576
  pilT Acinetobacter baumannii D1279779

57.647

100

0.576
  pilT Legionella pneumophila strain Lp02

55.952

98.824

0.553
  pilT Legionella pneumophila strain ERS1305867

55.952

98.824

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.695

96.471

0.479
  pilU Vibrio cholerae strain A1552

43.284

98.529

0.426
  pilU Pseudomonas stutzeri DSM 10701

41.493

98.529

0.409
  pilU Acinetobacter baylyi ADP1

39.583

98.824

0.391

Multiple sequence alignment