Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAIM57_RS04505 Genome accession   NZ_CP154587
Coordinates   913380..914360 (+) Length   326 a.a.
NCBI ID   WP_257651811.1    Uniprot ID   -
Organism   Citrobacter freundii strain NFYY361     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 908380..919360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIM57_RS04475 (AAIM57_04475) ansB 908894..909940 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  AAIM57_RS04480 (AAIM57_04480) hemW 910056..911192 (-) 1137 WP_204583285.1 radical SAM family heme chaperone HemW -
  AAIM57_RS04485 (AAIM57_04485) - 911185..911778 (-) 594 WP_003027104.1 XTP/dITP diphosphatase -
  AAIM57_RS04490 (AAIM57_04490) yggU 911786..912076 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  AAIM57_RS04495 (AAIM57_04495) - 912073..912639 (-) 567 WP_003027097.1 YggT family protein -
  AAIM57_RS04500 (AAIM57_04500) - 912658..913362 (-) 705 WP_071687445.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAIM57_RS04505 (AAIM57_04505) pilT 913380..914360 (+) 981 WP_257651811.1 type IV pilus twitching motility protein PilT Machinery gene
  AAIM57_RS04510 (AAIM57_04510) ruvX 914357..914773 (-) 417 WP_257651827.1 Holliday junction resolvase RuvX -
  AAIM57_RS04515 (AAIM57_04515) - 914773..915336 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  AAIM57_RS04520 (AAIM57_04520) gshB 915448..916395 (-) 948 WP_003027083.1 glutathione synthase -
  AAIM57_RS04525 (AAIM57_04525) rsmE 916415..917146 (-) 732 WP_003027080.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAIM57_RS04530 (AAIM57_04530) endA 917220..917927 (-) 708 WP_016150969.1 deoxyribonuclease I -
  AAIM57_RS04535 (AAIM57_04535) - 918022..918519 (-) 498 WP_003838223.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35955.04 Da        Isoelectric Point: 6.3638

>NTDB_id=993636 AAIM57_RS04505 WP_257651811.1 913380..914360(+) (pilT) [Citrobacter freundii strain NFYY361]
MNMEEIVALSVKHNVSDLHLCNAWPARWRRHGKVESAPFTAPDVENLLMCWLSEQQQVQLQEQGQIDFAVTLTDSRRLRA
SAFVHLQGTSLALRLLPLDCPHLDDLQPPAFIPELLHSENGLILVTGATGSGKSTTLAAMVEYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGAHCASFATGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDS
FAATEKDPVRNQLADSLRAVLTQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=993636 AAIM57_RS04505 WP_257651811.1 913380..914360(+) (pilT) [Citrobacter freundii strain NFYY361]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCAGACATGGAAAAGTCGAAAGCGCACCGTTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTTGCAGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTGACGGACTCCCGGCGGCTGCGCGCC
AGCGCATTTGTCCATCTGCAGGGAACCTCGCTGGCGCTAAGACTGCTACCGCTGGATTGTCCCCATTTAGACGATCTTCA
GCCTCCCGCGTTCATACCTGAACTGCTTCACAGTGAAAACGGGTTGATTCTGGTGACAGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCTGCGATGGTGGAGTATCTTAATCAGCATATTGAGGGGCACATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGAGCACACTGCGCCTCTTTCGCCACCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTGACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTGGATTCC
TTTGCAGCAACAGAGAAAGATCCTGTGCGTAACCAACTGGCAGACAGCCTGCGGGCGGTTCTTACGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAACTGCTTGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTACAGCAGCGA
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

45.652

98.773

0.451
  pilT Legionella pneumophila strain ERS1305867

45.652

98.773

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.956

97.239

0.408
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362

Multiple sequence alignment