Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LA521A_RS03300 Genome accession   NZ_AP027041
Coordinates   744371..745483 (-) Length   370 a.a.
NCBI ID   WP_281780962.1    Uniprot ID   -
Organism   Lysobacter auxotrophicus strain 5-21a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739371..750483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA521A_RS03270 (LA521A_06540) - 739951..741150 (-) 1200 WP_281780957.1 (Fe-S)-binding protein -
  LA521A_RS03275 (LA521A_06550) rplM 741322..741750 (+) 429 WP_281780958.1 50S ribosomal protein L13 -
  LA521A_RS03280 (LA521A_06560) rpsI 741777..742169 (+) 393 WP_281780959.1 30S ribosomal protein S9 -
  LA521A_RS03290 (LA521A_06570) - 742503..743492 (+) 990 WP_281780960.1 fumarylacetoacetate hydrolase family protein -
  LA521A_RS03295 (LA521A_06580) maiA 743552..744217 (+) 666 WP_281780961.1 maleylacetoacetate isomerase -
  LA521A_RS03300 (LA521A_06590) pilU 744371..745483 (-) 1113 WP_281780962.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LA521A_RS03305 (LA521A_06600) - 745713..745970 (+) 258 WP_281780963.1 DUF4398 domain-containing protein -
  LA521A_RS03310 (LA521A_06610) - 745967..746851 (+) 885 WP_281780964.1 OmpA family protein -
  LA521A_RS03315 (LA521A_06620) - 747241..747459 (+) 219 WP_115841330.1 YdcH family protein -
  LA521A_RS03320 (LA521A_06630) - 747718..749094 (+) 1377 WP_281780965.1 pyridoxal-phosphate dependent enzyme -
  LA521A_RS03325 (LA521A_06640) - 749094..750038 (+) 945 WP_281780966.1 2OG-Fe(II) oxygenase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41084.40 Da        Isoelectric Point: 5.9389

>NTDB_id=99224 LA521A_RS03300 WP_281780962.1 744371..745483(-) (pilU) [Lysobacter auxotrophicus strain 5-21a]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPEAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSNISGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLRAVVSQRLVVGTDGRRVPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEEGMESFDQCL
FRLHKEGRIDMEEALKAADSREGLALKFRLSEGSTGEHDPYADIYDTATQ

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=99224 LA521A_RS03300 WP_281780962.1 744371..745483(-) (pilU) [Lysobacter auxotrophicus strain 5-21a]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACGACGGGCGCACCGGTTTA
TATCAAGGTCGAAGGCAAGCTGTACCCCCTCGGCAATACGGGCCTGCCTCCGGGCATGGTCAAGAAGATCGCCTACTCCC
TGATGGACGAGGGACAGGTTCCGCTTTTCGAGCGTGACCTCGAACTGAACATGGCGCTCGCGCTGCCCGAAGCCGGCCGG
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGTGCCATCCGCAGCGTGATCCCGTCGATCGA
GGAACTGCAGCTGCCGCAGGTGCTCAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTCGCGTCGATGATCGACTACCGCAACAGCAATATTTCCGGTCACATCCTCACCATCGAGGAT
CCGATCGAATATCTGCACAAGCACAAGAAGTCGATCGTGAACCAGCGCGAAGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCACCGGACGTCATCCTCATCGGCGAAATCCTCGACGCCACGACGATGGAAGCCG
CGATCGCGTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTGGAACGCATC
CTCAACTTCTTCCCGGAAGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTGCGCGCGGTGGTCAGCCAGCG
ACTGGTCGTCGGCACCGATGGCCGTCGCGTGCCGGCGGCGGAAGTGCTGATCAACACGCCGCACATCCGCGACCTCATGC
GTCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTGCACAAGGAAGGCCGCATCGACATGGAAGAGGCGCTCAAGGCCGCCGACTCCCGCGAAGGCCTGGCGCTCAA
GTTCCGCCTGTCGGAAGGCTCCACCGGCGAGCACGATCCGTACGCGGACATCTACGACACGGCGACGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.811

97.027

0.503
  pilU Acinetobacter baylyi ADP1

48.588

95.676

0.465
  pilU Vibrio cholerae strain A1552

48.023

95.676

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.251

96.757

0.438
  pilT Acinetobacter baumannii strain A118

41.57

92.973

0.386
  pilT Acinetobacter nosocomialis M2

41.57

92.973

0.386
  pilT Acinetobacter baumannii D1279779

41.57

92.973

0.386
  pilT Pseudomonas aeruginosa PAK

42.012

91.351

0.384
  pilT Acinetobacter baylyi ADP1

41.279

92.973

0.384
  pilT Pseudomonas stutzeri DSM 10701

40.698

92.973

0.378
  pilT Legionella pneumophila strain Lp02

40.828

91.351

0.373
  pilT Legionella pneumophila strain ERS1305867

40.828

91.351

0.373
  pilT Neisseria gonorrhoeae MS11

39.481

93.784

0.37
  pilT Neisseria meningitidis 8013

39.481

93.784

0.37
  pilT Vibrio cholerae strain A1552

40.541

90

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90

0.365

Multiple sequence alignment