Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAGQ96_RS17590 Genome accession   NZ_CP152417
Coordinates   3846734..3847729 (-) Length   331 a.a.
NCBI ID   WP_342754173.1    Uniprot ID   -
Organism   Pantoea sp. MBD-2R     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3841734..3852729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGQ96_RS17560 (AAGQ96_17560) - 3842474..3843016 (+) 543 WP_147199691.1 SprT family zinc-dependent metalloprotease -
  AAGQ96_RS17565 (AAGQ96_17565) endA 3843101..3843814 (+) 714 WP_147199692.1 deoxyribonuclease I -
  AAGQ96_RS17570 (AAGQ96_17570) rsmE 3843890..3844621 (+) 732 WP_342754172.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAGQ96_RS17575 (AAGQ96_17575) gshB 3844709..3845653 (+) 945 WP_147199694.1 glutathione synthase -
  AAGQ96_RS17580 (AAGQ96_17580) - 3845746..3846309 (+) 564 WP_147199695.1 YqgE/AlgH family protein -
  AAGQ96_RS17585 (AAGQ96_17585) ruvX 3846309..3846731 (+) 423 WP_174822366.1 Holliday junction resolvase RuvX -
  AAGQ96_RS17590 (AAGQ96_17590) pilT 3846734..3847729 (-) 996 WP_342754173.1 type IV pilus twitching motility protein PilT Machinery gene
  AAGQ96_RS17595 (AAGQ96_17595) - 3847745..3848452 (+) 708 WP_342754174.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAGQ96_RS17600 (AAGQ96_17600) - 3848461..3849015 (+) 555 WP_342754175.1 YggT family protein -
  AAGQ96_RS17605 (AAGQ96_17605) - 3849041..3849634 (+) 594 WP_342754176.1 XTP/dITP diphosphatase -
  AAGQ96_RS17610 (AAGQ96_17610) hemW 3849627..3850766 (+) 1140 WP_342754177.1 radical SAM family heme chaperone HemW -
  AAGQ96_RS17615 (AAGQ96_17615) - 3850828..3851541 (-) 714 WP_147199702.1 DUF2884 domain-containing protein -
  AAGQ96_RS17620 (AAGQ96_17620) - 3851605..3851931 (-) 327 WP_147199703.1 YggL family protein -
  AAGQ96_RS17625 (AAGQ96_17625) trmB 3851931..3852650 (-) 720 WP_342754178.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 35909.27 Da        Isoelectric Point: 8.3430

>NTDB_id=989039 AAGQ96_RS17590 WP_342754173.1 3846734..3847729(-) (pilT) [Pantoea sp. MBD-2R]
MDIEKIVALSVKHNAGDLHLCSGHSPFWRRSGHLEKIADMPLIDDTQLQAVARRWLDAGQQAQFEQAGQIDFALSLPGGT
RLRANFFRQRQGVSLALRVIASDCPALASLRLPPVVSELLRLQDGLVLVTGATGSGKSTTLAALIGAINQQQKRHILTLE
DPIEFIHRSQQSLIQQREVGLHCRSFSAGLRAALREDPDVILLGELRDGDTIQQALTAAETGHLVLATLHTRGAAQVIDR
LVDVFPSAEKNLIRSQLAGSLKAAIAQKLVPDKTQGRIALFEVLVNTPAVASLIREGKTHQLPGVLQTGMQSGMQTFEQS
LSERQQAGLLP

Nucleotide


Download         Length: 996 bp        

>NTDB_id=989039 AAGQ96_RS17590 WP_342754173.1 3846734..3847729(-) (pilT) [Pantoea sp. MBD-2R]
ATGGATATTGAGAAGATCGTGGCGCTTAGTGTAAAGCATAACGCCGGGGATCTGCACCTTTGTAGTGGTCATTCTCCGTT
CTGGCGCCGATCCGGGCATCTGGAAAAAATAGCCGATATGCCACTCATTGACGACACGCAGTTGCAGGCGGTTGCGCGAC
GCTGGCTGGACGCAGGGCAGCAGGCGCAGTTTGAGCAGGCGGGGCAAATCGATTTTGCCCTGTCGCTGCCTGGCGGAACG
CGTCTGCGCGCCAATTTTTTCCGGCAGCGGCAGGGCGTTTCTCTGGCACTGCGAGTGATCGCCAGCGACTGTCCTGCACT
GGCCAGCCTGCGTCTGCCGCCTGTTGTCAGCGAACTGTTGCGTCTTCAGGATGGCCTGGTGCTGGTAACAGGCGCGACCG
GAAGCGGCAAATCCACCACGCTGGCGGCGTTGATTGGGGCGATTAATCAGCAGCAAAAACGCCATATTTTGACGCTGGAA
GATCCGATTGAATTTATTCACCGTAGCCAGCAATCGCTTATCCAGCAGCGGGAAGTCGGTCTGCACTGTCGCTCTTTTAG
CGCGGGGCTGAGGGCGGCACTGCGTGAAGACCCGGACGTCATCTTACTGGGCGAACTGCGTGACGGTGATACCATACAGC
AGGCCCTGACCGCTGCGGAAACCGGGCATCTGGTGCTGGCAACGCTGCATACCCGGGGCGCGGCTCAGGTCATTGACAGG
TTAGTAGACGTGTTTCCCAGCGCGGAAAAAAACCTCATCCGTAGCCAGCTGGCTGGCAGCCTGAAAGCGGCCATTGCGCA
AAAGCTGGTGCCTGACAAGACGCAGGGTAGAATCGCTTTATTTGAAGTGTTAGTGAATACGCCAGCGGTAGCGAGCCTGA
TTCGGGAAGGTAAAACGCATCAGTTGCCCGGCGTGTTGCAAACCGGTATGCAGTCCGGCATGCAAACCTTCGAACAGAGC
CTGAGTGAGCGTCAGCAGGCAGGTTTGCTGCCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.036

100

0.48
  pilT Neisseria gonorrhoeae MS11

47.734

100

0.477
  pilT Vibrio cholerae strain A1552

48.125

96.677

0.465
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.125

96.677

0.465
  pilT Pseudomonas stutzeri DSM 10701

46.364

99.698

0.462
  pilT Pseudomonas aeruginosa PAK

45.455

99.698

0.453
  pilT Legionella pneumophila strain Lp02

46.13

97.583

0.45
  pilT Legionella pneumophila strain ERS1305867

46.13

97.583

0.45
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.82

97.583

0.447
  pilT Acinetobacter baylyi ADP1

44.545

99.698

0.444
  pilT Acinetobacter baumannii D1279779

44.86

96.979

0.435
  pilT Acinetobacter baumannii strain A118

44.86

96.979

0.435
  pilT Acinetobacter nosocomialis M2

44.86

96.979

0.435
  pilU Pseudomonas stutzeri DSM 10701

40.994

97.281

0.399
  pilU Vibrio cholerae strain A1552

39.506

97.885

0.387
  pilU Acinetobacter baylyi ADP1

38.08

97.583

0.372

Multiple sequence alignment