Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MHB48_RS13290 Genome accession   NZ_CP152052
Coordinates   2767996..2769036 (-) Length   346 a.a.
NCBI ID   WP_342598511.1    Uniprot ID   -
Organism   Psychrobacillus sp. FSL H8-0483     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2762996..2774036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB48_RS13265 (MHB48_13265) - 2763845..2764531 (-) 687 WP_342598507.1 potassium transporter -
  MHB48_RS13270 (MHB48_13270) - 2764531..2765124 (-) 594 WP_342598508.1 hypothetical protein -
  MHB48_RS13275 (MHB48_13275) pilM 2765124..2766098 (-) 975 WP_342598509.1 pilus assembly protein PilM -
  MHB48_RS13280 (MHB48_13280) - 2766222..2766692 (-) 471 WP_342601382.1 type II secretion system protein -
  MHB48_RS13285 (MHB48_13285) - 2766791..2767996 (-) 1206 WP_342598510.1 type II secretion system F family protein -
  MHB48_RS13290 (MHB48_13290) pilT 2767996..2769036 (-) 1041 WP_342598511.1 type IV pilus twitching motility protein PilT Machinery gene
  MHB48_RS13295 (MHB48_13295) - 2769048..2770712 (-) 1665 WP_342598512.1 ATPase, T2SS/T4P/T4SS family -
  MHB48_RS13300 (MHB48_13300) - 2770734..2772098 (-) 1365 WP_342598513.1 VanW family protein -
  MHB48_RS13305 (MHB48_13305) - 2772496..2772978 (+) 483 WP_342598514.1 type II secretion system protein -
  MHB48_RS13310 (MHB48_13310) - 2772996..2773439 (+) 444 WP_342598515.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38245.75 Da        Isoelectric Point: 6.1821

>NTDB_id=986981 MHB48_RS13290 WP_342598511.1 2767996..2769036(-) (pilT) [Psychrobacillus sp. FSL H8-0483]
MKISIIDLLEKSFHDRASDLHITVGLAPVFRVNGNLQAYGESTLHEDDTRAMLNEILPPEKLQEFEENGELDFKYTLPNL
CHFRINAFHQQDQVAIAVRMIDAKIPAIETLGMPNVLYTLADKPQGLILVTGPTGSGKSTTLASMIDYINTTKSKHIITL
EDPIEYVHTHKKSVINQREVGSDTQSFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVMATLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIISQRLFVRNDAKGRVAATEILVQTPSVANLIRNEKVHQIQNVMQTSRALGMHTLE
SSIQTLVTSGKISLESAKPFMDVGEY

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=986981 MHB48_RS13290 WP_342598511.1 2767996..2769036(-) (pilT) [Psychrobacillus sp. FSL H8-0483]
ATGAAAATTTCGATTATAGACTTATTAGAAAAATCATTTCATGATCGAGCATCGGATCTTCACATTACGGTTGGATTGGC
TCCTGTATTTAGAGTTAATGGGAATCTACAAGCTTATGGGGAATCTACATTGCATGAAGATGATACAAGAGCGATGTTGA
ATGAAATATTACCCCCTGAGAAATTGCAGGAGTTCGAAGAAAATGGTGAACTTGATTTCAAATATACACTTCCTAATCTA
TGTCATTTTCGGATCAATGCTTTTCATCAACAAGACCAAGTTGCCATAGCAGTTCGCATGATTGATGCAAAGATACCGGC
GATTGAAACACTTGGAATGCCTAATGTCTTATATACTTTAGCAGATAAACCTCAAGGACTAATTCTAGTAACTGGTCCTA
CTGGGTCTGGCAAATCAACGACACTTGCATCCATGATAGATTATATCAACACAACAAAATCGAAGCACATTATTACGTTA
GAAGACCCAATTGAATACGTGCATACGCATAAGAAGTCAGTCATTAATCAACGAGAAGTCGGTTCGGATACGCAAAGCTT
TGCCAATGGATTACGAGCTGCACTCCGTCAAGATCCCGATGTTATTTTAGTTGGTGAAATGCGAGATTTAGAAACAATTT
CTACCGCCATTACAGCAGCGGAAACGGGTCATCTAGTCATGGCAACACTTCATACAAGTAGTGCTCCAACGACGATCGAT
CGAATTATTGATGTTTTTCCACCACACCAACAAGGGCAAATTCGCATTCAACTAGCAAACGTACTACAAGGGATCATTTC
TCAAAGACTCTTTGTTAGAAACGATGCAAAAGGACGTGTCGCGGCAACGGAGATTTTAGTTCAAACACCGTCTGTTGCCA
ATCTAATACGAAATGAAAAGGTGCATCAAATTCAAAATGTCATGCAAACAAGTCGAGCACTTGGCATGCATACACTCGAA
TCATCGATTCAAACTTTGGTCACTTCAGGTAAGATTTCGCTAGAATCAGCAAAACCGTTTATGGATGTAGGTGAGTATTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.395

93.642

0.5
  pilT Pseudomonas aeruginosa PAK

49.405

97.11

0.48
  pilT Pseudomonas stutzeri DSM 10701

49.405

97.11

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

95.376

0.471
  pilT Vibrio cholerae strain A1552

49.394

95.376

0.471
  pilT Acinetobacter baumannii D1279779

48.214

97.11

0.468
  pilT Neisseria meningitidis 8013

48.214

97.11

0.468
  pilT Acinetobacter nosocomialis M2

48.214

97.11

0.468
  pilT Acinetobacter baumannii strain A118

48.214

97.11

0.468
  pilT Neisseria gonorrhoeae MS11

47.917

97.11

0.465
  pilT Legionella pneumophila strain Lp02

48.06

96.821

0.465
  pilT Legionella pneumophila strain ERS1305867

48.06

96.821

0.465
  pilT Acinetobacter baylyi ADP1

47.619

97.11

0.462
  pilU Pseudomonas stutzeri DSM 10701

42.368

92.775

0.393
  pilU Vibrio cholerae strain A1552

42.236

93.064

0.393
  pilU Acinetobacter baylyi ADP1

40.361

95.954

0.387

Multiple sequence alignment