Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MHI37_RS19720 Genome accession   NZ_CP152049
Coordinates   4505262..4506299 (-) Length   345 a.a.
NCBI ID   WP_076339781.1    Uniprot ID   A0A1R1CH95
Organism   Paenibacillus sp. FSL H8-0548     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4500262..4511299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI37_RS19695 (MHI37_19695) - 4501564..4502031 (+) 468 WP_076339777.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHI37_RS19700 (MHI37_19700) - 4502053..4502562 (+) 510 WP_076339778.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHI37_RS19705 (MHI37_19705) - 4502566..4503339 (-) 774 WP_083676572.1 A24 family peptidase -
  MHI37_RS19710 (MHI37_19710) - 4503410..4503766 (-) 357 WP_218638959.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHI37_RS19715 (MHI37_19715) - 4504017..4505228 (-) 1212 WP_076339780.1 type II secretion system F family protein -
  MHI37_RS19720 (MHI37_19720) pilT 4505262..4506299 (-) 1038 WP_076339781.1 type IV pilus twitching motility protein PilT Machinery gene
  MHI37_RS19725 (MHI37_19725) - 4506332..4507999 (-) 1668 WP_076339782.1 ATPase, T2SS/T4P/T4SS family -
  MHI37_RS19730 (MHI37_19730) - 4508386..4509606 (+) 1221 WP_076339784.1 MFS transporter -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38140.90 Da        Isoelectric Point: 6.5956

>NTDB_id=986848 MHI37_RS19720 WP_076339781.1 4505262..4506299(-) (pilT) [Paenibacillus sp. FSL H8-0548]
MQAEELLYLAHSKQASDIHITVNSPVIFRIHGELRPINDTLLKPNDTLELAKQFMTEERYETLQQNGDVDFSYGVAGLAR
FRINAYKQRGSVGLAIRIVPSVIPTMEELRLPRIAAEFAKKPQGLLLVTGPTGSGKSTTLASIIDHMNRTENSHIITLED
PIEFIYDHKKSMVNQREVGLDTPSFSHGLRSALRQDPDIILVGELRDLETISTAISAAETGHLVFGTLHTADAPQTIDRI
IDVFPPAMQAQIRIQLAAVLLGVISQRLLPRADGKGRTAAFEVLVNTPAVANLIRVEKIYQIRSVMQTGKALGMQTMEYA
LRELVQQGTVKIQDARDAVFGFGEL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=986848 MHI37_RS19720 WP_076339781.1 4505262..4506299(-) (pilT) [Paenibacillus sp. FSL H8-0548]
ATGCAGGCAGAGGAATTGCTTTATTTAGCTCATTCAAAACAAGCGTCGGATATTCATATTACCGTTAATTCACCTGTTAT
TTTTCGAATTCATGGAGAATTAAGACCGATTAACGATACGCTATTAAAGCCAAATGACACACTTGAGCTTGCGAAACAGT
TCATGACCGAGGAACGTTATGAGACGCTTCAGCAAAATGGCGATGTTGATTTCTCCTATGGAGTTGCGGGTCTTGCCAGA
TTTCGGATTAACGCCTATAAGCAGCGAGGAAGCGTGGGACTTGCGATACGTATTGTACCAAGTGTAATCCCAACGATGGA
GGAGCTTCGGTTGCCGCGAATTGCAGCGGAGTTTGCGAAGAAGCCGCAGGGGCTGCTGCTTGTTACTGGACCTACGGGAA
GCGGGAAATCAACGACCCTGGCCTCTATCATTGATCATATGAATCGAACGGAAAATTCGCATATTATTACGCTGGAGGAT
CCGATCGAGTTTATCTATGATCATAAAAAATCGATGGTCAACCAACGAGAGGTTGGACTGGACACGCCCTCTTTCTCCCA
TGGCTTGCGATCAGCACTTAGGCAGGACCCTGATATCATACTTGTAGGCGAATTGCGGGATTTGGAGACGATCAGTACTG
CGATTAGTGCAGCAGAAACAGGTCATCTTGTGTTTGGGACACTGCATACCGCGGACGCTCCGCAAACGATTGACCGTATT
ATTGATGTTTTTCCACCTGCGATGCAGGCACAAATTAGAATACAGCTAGCCGCAGTTCTGTTAGGCGTTATTTCTCAGCG
ACTGTTGCCTAGAGCAGATGGAAAGGGGAGAACGGCTGCATTTGAAGTTCTGGTCAACACACCAGCCGTAGCCAATCTTA
TCCGAGTCGAGAAAATCTATCAGATTAGATCAGTTATGCAGACAGGCAAGGCGCTCGGGATGCAGACGATGGAATATGCA
CTAAGGGAGCTCGTCCAGCAGGGGACTGTGAAAATCCAAGATGCGCGTGATGCTGTTTTTGGATTTGGAGAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R1CH95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.012

93.623

0.487
  pilT Legionella pneumophila strain Lp02

48.974

98.841

0.484
  pilT Legionella pneumophila strain ERS1305867

48.974

98.841

0.484
  pilT Neisseria meningitidis 8013

48.81

97.391

0.475
  pilT Pseudomonas aeruginosa PAK

48.665

97.681

0.475
  pilT Neisseria gonorrhoeae MS11

48.512

97.391

0.472
  pilT Acinetobacter baumannii strain A118

47.929

97.971

0.47
  pilT Acinetobacter nosocomialis M2

47.929

97.971

0.47
  pilT Acinetobacter baumannii D1279779

47.929

97.971

0.47
  pilT Vibrio cholerae strain A1552

47.493

98.261

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.493

98.261

0.467
  pilT Pseudomonas stutzeri DSM 10701

47.478

97.681

0.464
  pilT Acinetobacter baylyi ADP1

47.321

97.391

0.461
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Acinetobacter baylyi ADP1

41.071

97.391

0.4
  pilU Vibrio cholerae strain A1552

42.284

93.913

0.397

Multiple sequence alignment