Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MHB51_RS06220 Genome accession   NZ_CP152046
Coordinates   1292936..1293979 (-) Length   347 a.a.
NCBI ID   WP_107942337.1    Uniprot ID   -
Organism   Metasolibacillus sp. FSL K6-0083     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1287936..1298979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB51_RS06195 (MHB51_06195) - 1288486..1289172 (-) 687 WP_342559232.1 potassium transporter -
  MHB51_RS06200 (MHB51_06200) - 1289172..1289771 (-) 600 WP_066171168.1 hypothetical protein -
  MHB51_RS06205 (MHB51_06205) pilM 1289768..1290745 (-) 978 WP_342559233.1 pilus assembly protein PilM -
  MHB51_RS06210 (MHB51_06210) - 1291196..1291633 (-) 438 WP_342559234.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHB51_RS06215 (MHB51_06215) - 1291734..1292939 (-) 1206 WP_066171175.1 type II secretion system F family protein -
  MHB51_RS06220 (MHB51_06220) pilT 1292936..1293979 (-) 1044 WP_107942337.1 type IV pilus twitching motility protein PilT Machinery gene
  MHB51_RS06225 (MHB51_06225) - 1293992..1295662 (-) 1671 WP_342559235.1 ATPase, T2SS/T4P/T4SS family -
  MHB51_RS06230 (MHB51_06230) - 1295681..1296946 (-) 1266 WP_342559236.1 VanW family protein -
  MHB51_RS06235 (MHB51_06235) - 1297537..1297995 (+) 459 WP_342559237.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHB51_RS06240 (MHB51_06240) - 1297997..1298437 (+) 441 WP_342559238.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38416.04 Da        Isoelectric Point: 6.8726

>NTDB_id=986709 MHB51_RS06220 WP_107942337.1 1292936..1293979(-) (pilT) [Metasolibacillus sp. FSL K6-0083]
MTHSIRELLMHAYEQKASDLHLTVGIPPMYRIDGKLAPYGNDMITHEQLNTMVQELIPNHKAQEYKDKGETDFNYSLESL
CRFRVNAYHQRNTSAVAARLISSEIPTIDSLNMPSVLFDLADKPQGLILVTGPTGSGKSTTLAAMIDYINTNKAKHIITL
EDPIEYLHTHKKSVVNQREVGVDTESFANGLRASLRQDPDIILVGEMRDLETISTAITAAETGHLVFGTLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIISQRLFVRKGKKGRVAATEILVAIPAVTNLIRNEKIHQIPSVMQTNRALGMHTLE
TSIQGLVSAGQIALEDARPFMNVGEYI

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=986709 MHB51_RS06220 WP_107942337.1 1292936..1293979(-) (pilT) [Metasolibacillus sp. FSL K6-0083]
ATGACACACTCAATTCGAGAATTATTAATGCATGCTTATGAGCAAAAGGCATCCGATTTACATTTAACTGTCGGTATTCC
ACCAATGTATCGTATTGACGGCAAGCTAGCTCCTTACGGTAATGACATGATTACACATGAGCAATTAAATACGATGGTTC
AGGAACTAATTCCAAACCATAAGGCGCAGGAATATAAGGATAAGGGGGAAACGGATTTTAATTATTCGTTAGAAAGCCTC
TGCCGATTTCGTGTGAATGCATATCATCAACGCAATACAAGTGCTGTTGCAGCTCGTTTAATTTCAAGTGAAATCCCAAC
AATCGACTCTTTAAATATGCCGAGTGTACTGTTCGATTTGGCGGACAAGCCTCAAGGTTTGATTTTAGTAACAGGTCCTA
CAGGCTCCGGGAAATCAACAACTTTAGCAGCAATGATTGACTATATTAATACGAATAAAGCGAAGCATATTATTACGCTT
GAAGACCCGATTGAGTATTTGCATACACATAAAAAATCAGTCGTCAATCAACGCGAAGTCGGTGTGGATACGGAAAGCTT
TGCAAACGGCTTGCGTGCATCATTACGTCAAGACCCTGATATTATTTTAGTCGGGGAGATGCGTGACTTAGAAACGATTT
CGACTGCTATTACCGCGGCAGAAACTGGGCATCTCGTTTTTGGCACATTGCATACATCAAGTGCTCCAACGACGATTGAC
CGCATTATTGACGTATTTCCACCGCATCAGCAGGGACAAATACGCATTCAGCTAGCCAATGTTTTACAAGGTATTATTTC
CCAACGTCTATTTGTGCGCAAAGGTAAAAAGGGGCGTGTGGCAGCAACGGAAATACTTGTTGCAATTCCCGCTGTGACAA
ATTTAATTCGCAATGAGAAAATTCATCAAATTCCAAGTGTGATGCAAACGAATCGAGCACTTGGCATGCATACGCTGGAA
ACGTCAATCCAAGGGCTTGTGTCCGCTGGGCAAATTGCGTTGGAGGATGCGCGTCCATTTATGAATGTAGGTGAATACAT
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

50.898

96.254

0.49
  pilT Neisseria meningitidis 8013

50.898

96.254

0.49
  pilT Acinetobacter baumannii strain A118

50.599

96.254

0.487
  pilT Pseudomonas aeruginosa PAK

50.599

96.254

0.487
  pilT Acinetobacter baumannii D1279779

50.599

96.254

0.487
  pilT Acinetobacter baylyi ADP1

50.599

96.254

0.487
  pilT Neisseria gonorrhoeae MS11

50.599

96.254

0.487
  pilT Vibrio cholerae strain A1552

50.915

94.524

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.915

94.524

0.481
  pilT Pseudomonas stutzeri DSM 10701

49.701

96.254

0.478
  pilT Legionella pneumophila strain ERS1305867

50.152

94.813

0.476
  pilT Legionella pneumophila strain Lp02

50.152

94.813

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.932

92.795

0.473
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.389

0.392
  pilU Vibrio cholerae strain A1552

41.615

92.795

0.386
  pilU Acinetobacter baylyi ADP1

37.952

95.677

0.363

Multiple sequence alignment