Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MHH37_RS01560 Genome accession   NZ_CP152038
Coordinates   319214..320257 (+) Length   347 a.a.
NCBI ID   WP_342574715.1    Uniprot ID   -
Organism   Solibacillus sp. FSL K6-1781     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314214..325257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH37_RS01540 (MHH37_01540) - 314778..315221 (-) 444 WP_342574711.1 hypothetical protein -
  MHH37_RS01545 (MHH37_01545) - 315233..315715 (-) 483 WP_342574712.1 hypothetical protein -
  MHH37_RS01550 (MHH37_01550) - 316130..317521 (+) 1392 WP_342574713.1 VanW family protein -
  MHH37_RS01555 (MHH37_01555) - 317537..319201 (+) 1665 WP_342574714.1 ATPase, T2SS/T4P/T4SS family -
  MHH37_RS01560 (MHH37_01560) pilT 319214..320257 (+) 1044 WP_342574715.1 type IV pilus twitching motility protein PilT Machinery gene
  MHH37_RS01565 (MHH37_01565) - 320254..321462 (+) 1209 WP_342574716.1 type II secretion system F family protein -
  MHH37_RS01570 (MHH37_01570) - 321572..322015 (+) 444 WP_342574717.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MHH37_RS01575 (MHH37_01575) pilM 322140..323117 (+) 978 WP_342574718.1 pilus assembly protein PilM -
  MHH37_RS01580 (MHH37_01580) - 323114..323704 (+) 591 WP_342574719.1 PilN domain-containing protein -
  MHH37_RS01585 (MHH37_01585) - 323704..324414 (+) 711 WP_342574720.1 potassium transporter -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38618.15 Da        Isoelectric Point: 6.5931

>NTDB_id=986253 MHH37_RS01560 WP_342574715.1 319214..320257(+) (pilT) [Solibacillus sp. FSL K6-1781]
MTLNIQDLLQRAYDEKASDLHVTTGIPPVYRVNGQLKQYGNEIVTEEIVTRMLQDILPKYKLGEFEDKGETDFNYSLEDL
CRFRVNAYHQRNASAIAARLISSKIPTIESLNLPRVLYDLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETKSRHIITL
EDPIEYLHSHKKSVVNQREIGVDTGSFANGLRASLRQDPDIILVGEMRDLETISTAITAAETGHLVFGTLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIISQRLFIRKDKTGRVAATEILVSIPAVTNLIRNEKIYQIPSIMQTNRALGMHTLE
TSIQALVSSGKVSLEEVRPYLNVGDYS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=986253 MHH37_RS01560 WP_342574715.1 319214..320257(+) (pilT) [Solibacillus sp. FSL K6-1781]
ATGACATTGAACATACAAGATTTATTGCAACGTGCGTATGACGAAAAAGCATCCGATTTGCATGTCACTACCGGCATCCC
TCCTGTTTACCGTGTAAATGGTCAATTGAAGCAATATGGGAATGAAATTGTTACGGAAGAAATAGTAACCCGCATGCTAC
AGGATATTTTACCGAAGTATAAATTAGGGGAATTTGAGGATAAAGGTGAAACCGATTTTAACTATTCACTAGAAGATTTA
TGCCGCTTCCGTGTAAATGCGTATCATCAGCGAAATGCAAGTGCGATTGCTGCGCGTTTGATATCCAGTAAAATTCCAAC
AATTGAATCGTTAAATTTGCCTAGAGTACTGTACGATTTAGCTGAAAAACCACAAGGACTCATTTTAGTAACTGGTCCAA
CTGGTTCAGGTAAGTCAACAACACTCGCGGCAATGATTGACTATATTAATGAAACCAAATCAAGACATATTATTACGCTG
GAAGACCCGATCGAATATTTGCATTCCCATAAAAAATCGGTAGTGAACCAGCGTGAAATTGGAGTCGATACAGGATCATT
TGCGAATGGTTTACGTGCATCACTCCGTCAAGATCCCGATATTATTCTAGTTGGGGAAATGCGAGATTTAGAGACAATTT
CAACAGCAATTACGGCAGCGGAAACAGGGCATTTAGTGTTTGGCACACTTCATACGTCAAGTGCACCGACAACAATTGAC
CGTATAATCGATGTGTTCCCGCCTCATCAGCAAGGGCAAATTCGGATTCAATTAGCCAACGTATTGCAAGGAATTATTTC
ACAGCGCTTATTCATTCGGAAAGATAAAACGGGCAGGGTTGCCGCAACAGAAATTCTGGTAAGTATTCCAGCTGTAACAA
ATTTGATACGAAATGAAAAAATTTATCAAATTCCAAGTATTATGCAGACTAACCGAGCGTTAGGGATGCATACGTTAGAG
ACATCCATACAAGCGCTTGTTTCTTCCGGGAAAGTTTCGTTGGAGGAAGTACGGCCATATTTGAATGTGGGTGATTACAG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

50

96.83

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.543

93.372

0.481
  pilT Acinetobacter baumannii strain A118

49.702

96.83

0.481
  pilT Pseudomonas aeruginosa PAK

49.702

96.83

0.481
  pilT Neisseria meningitidis 8013

49.702

96.83

0.481
  pilT Acinetobacter baumannii D1279779

49.702

96.83

0.481
  pilT Acinetobacter baylyi ADP1

49.405

96.83

0.478
  pilT Neisseria gonorrhoeae MS11

49.405

96.83

0.478
  pilT Legionella pneumophila strain Lp02

49.849

95.389

0.476
  pilT Legionella pneumophila strain ERS1305867

49.849

95.389

0.476
  pilT Vibrio cholerae strain A1552

48.81

96.83

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.81

96.83

0.473
  pilT Pseudomonas stutzeri DSM 10701

48.512

96.83

0.47
  pilU Vibrio cholerae strain A1552

42.138

91.643

0.386
  pilU Pseudomonas stutzeri DSM 10701

39.521

96.254

0.38
  pilU Acinetobacter baylyi ADP1

38.253

95.677

0.366

Multiple sequence alignment