Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKY09_RS07460 Genome accession   NZ_CP152020
Coordinates   1534905..1535945 (+) Length   346 a.a.
NCBI ID   WP_342567998.1    Uniprot ID   -
Organism   Psychrobacillus sp. FSL K6-4046     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1529905..1540945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY09_RS07440 (MKY09_07440) - 1530758..1531255 (-) 498 WP_251553460.1 hypothetical protein -
  MKY09_RS07445 (MKY09_07445) - 1531248..1531649 (-) 402 WP_251553461.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY09_RS07450 (MKY09_07450) - 1531901..1533208 (+) 1308 WP_342567996.1 VanW family protein -
  MKY09_RS07455 (MKY09_07455) - 1533230..1534894 (+) 1665 WP_342567997.1 ATPase, T2SS/T4P/T4SS family -
  MKY09_RS07460 (MKY09_07460) pilT 1534905..1535945 (+) 1041 WP_342567998.1 type IV pilus twitching motility protein PilT Machinery gene
  MKY09_RS07465 (MKY09_07465) - 1535945..1537150 (+) 1206 WP_251553467.1 type II secretion system F family protein -
  MKY09_RS07470 (MKY09_07470) - 1537182..1537706 (+) 525 WP_251553468.1 type II secretion system protein -
  MKY09_RS07475 (MKY09_07475) pilM 1537825..1538802 (+) 978 WP_251553469.1 pilus assembly protein PilM -
  MKY09_RS07480 (MKY09_07480) - 1538805..1539392 (+) 588 WP_251553470.1 hypothetical protein -
  MKY09_RS07485 (MKY09_07485) - 1539389..1540111 (+) 723 WP_342567999.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38278.81 Da        Isoelectric Point: 6.1243

>NTDB_id=985236 MKY09_RS07460 WP_342567998.1 1534905..1535945(+) (pilT) [Psychrobacillus sp. FSL K6-4046]
MDLNIFDLLEKAYQDKASDLHLTVGLAPVFRVHGKLENFGDYQLQNNDTKKMLEDLLDTDALHEFSQKGELDFSFALEDL
CRFRINAYLQQNNVAIAIRIIESSIPSVEDLGMPNVLYSLADKPQGLILVTGPTGSGKSTTLAAMIDYINATKSKHIITL
EDPIEYVHRHKKSIVNQREVGSDTQSFANGLRAALRQDPDVILVGEMRDLETISTAITAAETGHLVMATLHTSSAPTTID
RIIDVFPPHQQGQIRIQLANVLQGIVSQRLFSKVNGAGRAVATEILIQTPSVTNLIRNEKIHQIKNVMQTSRALGMHTLE
SSIQQLLVTKQITLEAAKPFMNAGEF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=985236 MKY09_RS07460 WP_342567998.1 1534905..1535945(+) (pilT) [Psychrobacillus sp. FSL K6-4046]
ATGGATTTAAATATTTTTGACTTATTAGAAAAAGCATATCAAGATAAAGCCTCTGATCTTCATTTAACAGTTGGCCTGGC
GCCGGTTTTTCGAGTTCATGGGAAGCTTGAAAACTTTGGAGATTACCAGTTGCAGAATAATGACACTAAAAAAATGCTGG
AAGATCTGTTAGATACGGATGCTCTGCATGAGTTTTCACAAAAAGGAGAGCTGGACTTTAGTTTTGCTCTAGAGGACTTA
TGTAGATTTCGGATCAATGCGTATCTCCAACAAAATAACGTTGCCATTGCTATAAGAATAATTGAATCGAGCATACCTTC
AGTTGAAGATTTGGGAATGCCTAACGTCCTTTACTCACTTGCCGATAAACCTCAAGGTCTAATTCTTGTGACAGGACCTA
CTGGTTCAGGAAAGTCTACAACTTTAGCTGCTATGATCGATTATATTAACGCAACTAAATCAAAACATATTATTACGCTT
GAAGATCCGATTGAATATGTTCATAGACATAAGAAATCTATCGTCAATCAAAGAGAGGTTGGTTCGGATACTCAAAGTTT
TGCCAATGGATTGCGTGCAGCACTGCGTCAAGATCCCGATGTAATTCTAGTAGGAGAAATGCGAGATTTAGAAACTATCT
CTACAGCAATCACAGCAGCTGAAACGGGTCATTTAGTAATGGCTACATTACATACGAGTAGCGCACCAACTACCATAGAC
CGCATTATTGACGTGTTTCCTCCGCATCAGCAAGGTCAAATTCGAATCCAGCTTGCAAATGTCCTACAGGGAATTGTGTC
CCAACGTCTCTTCTCTAAGGTTAATGGAGCAGGCAGAGCTGTAGCGACTGAAATCTTAATCCAAACGCCGTCGGTTACTA
ACCTTATACGCAACGAGAAAATTCATCAAATCAAAAATGTGATGCAAACTAGTCGAGCGTTAGGAATGCATACCCTTGAA
TCATCTATTCAGCAGCTCCTAGTCACTAAACAAATTACTTTGGAGGCTGCGAAGCCGTTTATGAATGCAGGTGAATTTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.252

96.243

0.503
  pilT Legionella pneumophila strain Lp02

48.968

97.977

0.48
  pilT Legionella pneumophila strain ERS1305867

48.968

97.977

0.48
  pilT Pseudomonas stutzeri DSM 10701

49.107

97.11

0.477
  pilT Neisseria gonorrhoeae MS11

48.81

97.11

0.474
  pilT Neisseria meningitidis 8013

48.81

97.11

0.474
  pilT Pseudomonas aeruginosa PAK

48.214

97.11

0.468
  pilT Acinetobacter baylyi ADP1

46.939

99.133

0.465
  pilT Acinetobacter baumannii strain A118

47.024

97.11

0.457
  pilT Acinetobacter baumannii D1279779

47.024

97.11

0.457
  pilT Acinetobacter nosocomialis M2

47.024

97.11

0.457
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.308

93.931

0.454
  pilT Vibrio cholerae strain A1552

48.308

93.931

0.454
  pilU Vibrio cholerae strain A1552

42.943

96.243

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.121

95.376

0.402
  pilU Acinetobacter baylyi ADP1

39.645

97.688

0.387

Multiple sequence alignment