Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKY76_RS06120 Genome accession   NZ_CP151995
Coordinates   1288126..1289172 (+) Length   348 a.a.
NCBI ID   WP_048391346.1    Uniprot ID   -
Organism   Lysinibacillus sp. FSL P4-0201     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1283126..1294172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY76_RS06095 (MKY76_06095) - 1283599..1284048 (-) 450 WP_054550582.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY76_RS06100 (MKY76_06100) - 1284050..1284514 (-) 465 WP_112117554.1 type II secretion system protein -
  MKY76_RS06105 (MKY76_06105) - 1285095..1285427 (+) 333 WP_342557527.1 hypothetical protein -
  MKY76_RS06110 (MKY76_06110) - 1285554..1286426 (+) 873 WP_342557528.1 G5 domain-containing protein -
  MKY76_RS06115 (MKY76_06115) - 1286450..1288114 (+) 1665 WP_048391675.1 GspE/PulE family protein -
  MKY76_RS06120 (MKY76_06120) pilT 1288126..1289172 (+) 1047 WP_048391346.1 type IV pilus twitching motility protein PilT Machinery gene
  MKY76_RS06125 (MKY76_06125) - 1289169..1290374 (+) 1206 WP_036080216.1 type II secretion system F family protein -
  MKY76_RS06130 (MKY76_06130) - 1290500..1290976 (+) 477 WP_342557529.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY76_RS06135 (MKY76_06135) - 1291033..1291476 (+) 444 WP_257961758.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY76_RS06140 (MKY76_06140) pilM 1291589..1292563 (+) 975 WP_342557530.1 pilus assembly protein PilM -
  MKY76_RS06145 (MKY76_06145) - 1292563..1293156 (+) 594 WP_257961756.1 hypothetical protein -
  MKY76_RS06150 (MKY76_06150) - 1293153..1293845 (+) 693 WP_048391335.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38870.78 Da        Isoelectric Point: 7.2226

>NTDB_id=984045 MKY76_RS06120 WP_048391346.1 1288126..1289172(+) (pilT) [Lysinibacillus sp. FSL P4-0201]
MTAYSIVELLTRAFEEKASDLHLTKGIPPVYRIHGQLEHYGTIPLETEDLKAMVKVILPVHKQQEFEDKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYKLAEKPQGLILVTGPTGSGKSTTLAAMIDHINETRSKHIIT
LEDPIEYLHTHKKSVINQREIGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIVSQRLFVRKDVKGRIAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
EYSMQHLITTGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=984045 MKY76_RS06120 WP_048391346.1 1288126..1289172(+) (pilT) [Lysinibacillus sp. FSL P4-0201]
ATGACAGCATATTCAATCGTTGAATTATTAACACGTGCATTTGAAGAAAAAGCATCTGATCTTCATTTAACAAAAGGTAT
TCCACCAGTATATCGTATCCATGGGCAACTAGAGCATTACGGCACAATCCCACTTGAAACAGAGGATTTGAAGGCAATGG
TAAAGGTCATTCTTCCTGTCCATAAACAACAGGAATTTGAGGATAAGGGAGAAACAGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTCAATGCATACCATCAGCGAAATGAAGTGACGATTGCAGCTCGTTTAATTGAGGCTAAAATTCC
TTCTATTGAAGCACTCAATATGCCACAGGTGTTATATAAGCTTGCGGAAAAACCACAAGGCTTAATTTTAGTAACTGGCC
CAACAGGCTCAGGGAAATCAACTACCTTGGCAGCAATGATAGATCACATCAATGAAACAAGATCTAAACATATTATTACG
TTAGAGGATCCAATTGAATATTTGCATACACATAAGAAGTCTGTCATCAATCAACGTGAGATTGGTGTCGATACAATGAG
CTTTGCCAATGGTTTACGTGCATCATTACGTCAAGACCCAGATATTATTTTAGTTGGGGAGATGCGAGATTATGAAACCA
TTTCAACGGCTATTACGGCTGCTGAAACAGGGCATTTAGTTATGGCTACACTGCATACAAGTAGTGCACCAACGACGATC
GACCGTATTATCGATGTATTTCCACCGCATCAGCAGAGTCAAATTCGTGTGCAGCTTGCGAATGTACTACAAGGTATTGT
TTCTCAGCGTTTATTTGTACGTAAGGATGTAAAAGGGCGTATTGCTGCGACAGAAATTTTAGTACAGGCACCTGCTATTT
CTAATTTAATTCGTAACGAAAAAATTCACCAAATTCCAAGTGTGATGCAGACAAGTAAAGCATTGGGTATGCACACATTG
GAGTATTCCATGCAACACCTAATTACCACAGGTAAGATTTCACTAGAGGATGCAAGACCATATATGAACGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.083

96.552

0.503
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Neisseria gonorrhoeae MS11

49.701

95.977

0.477
  pilT Acinetobacter baumannii D1279779

49.401

95.977

0.474
  pilT Pseudomonas aeruginosa PAK

49.401

95.977

0.474
  pilT Acinetobacter nosocomialis M2

49.401

95.977

0.474
  pilT Acinetobacter baumannii strain A118

49.401

95.977

0.474
  pilT Vibrio cholerae strain A1552

48.503

95.977

0.466
  pilT Pseudomonas stutzeri DSM 10701

48.503

95.977

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.503

95.977

0.466
  pilT Legionella pneumophila strain Lp02

49.24

94.54

0.466
  pilT Legionella pneumophila strain ERS1305867

49.24

94.54

0.466
  pilT Acinetobacter baylyi ADP1

47.904

95.977

0.46
  pilU Pseudomonas stutzeri DSM 10701

42.296

95.115

0.402
  pilU Vibrio cholerae strain A1552

42.547

92.529

0.394
  pilU Acinetobacter baylyi ADP1

40.06

95.402

0.382

Multiple sequence alignment