Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NST76_RS05660 Genome accession   NZ_CP151994
Coordinates   1217080..1218126 (+) Length   348 a.a.
NCBI ID   WP_089932048.1    Uniprot ID   -
Organism   Lysinibacillus sp. FSL W7-1291     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1212080..1223126
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST76_RS05640 (NST76_05640) - 1212828..1213286 (-) 459 WP_180957770.1 type II secretion system protein -
  NST76_RS05645 (NST76_05645) - 1213291..1213737 (-) 447 WP_058843953.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NST76_RS05650 (NST76_05650) - 1214049..1215380 (+) 1332 WP_036128109.1 G5 domain-containing protein -
  NST76_RS05655 (NST76_05655) - 1215398..1217068 (+) 1671 WP_244504801.1 ATPase, T2SS/T4P/T4SS family -
  NST76_RS05660 (NST76_05660) pilT 1217080..1218126 (+) 1047 WP_089932048.1 type IV pilus twitching motility protein PilT Machinery gene
  NST76_RS05665 (NST76_05665) - 1218123..1219328 (+) 1206 WP_330493475.1 type II secretion system F family protein -
  NST76_RS05670 (NST76_05670) - 1219416..1219901 (+) 486 WP_036128097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NST76_RS05675 (NST76_05675) pilM 1220014..1220988 (+) 975 WP_330493476.1 pilus assembly protein PilM -
  NST76_RS05680 (NST76_05680) - 1220988..1221581 (+) 594 WP_036128090.1 PilN domain-containing protein -
  NST76_RS05685 (NST76_05685) - 1221578..1222267 (+) 690 WP_089932054.1 hypothetical protein -
  NST76_RS05690 (NST76_05690) - 1222335..1223087 (+) 753 WP_036128085.1 A24 family peptidase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38766.49 Da        Isoelectric Point: 6.8733

>NTDB_id=984007 NST76_RS05660 WP_089932048.1 1217080..1218126(+) (pilT) [Lysinibacillus sp. FSL W7-1291]
MTSYSIVELLTRAFEEKASDLHLTKGIPPVYRIHGQLEHYGADPLDSEELKGMVNAILPVHKQQEFEEKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYKLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETRSKHIIT
LEDPIEYLHTHKKSIINQREVGVDTMSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQSQIRVQLANVLQGIVSQRLFIRKDVKGRIAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
ESSMQHLITTGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=984007 NST76_RS05660 WP_089932048.1 1217080..1218126(+) (pilT) [Lysinibacillus sp. FSL W7-1291]
ATGACATCTTATTCAATCGTTGAATTATTAACACGTGCGTTTGAAGAAAAAGCATCGGATCTTCATTTAACAAAAGGTAT
TCCGCCAGTATATCGTATTCATGGGCAGTTGGAACATTACGGTGCAGATCCATTAGATTCTGAAGAATTAAAGGGAATGG
TAAATGCCATTCTTCCTGTACATAAACAACAGGAGTTTGAGGAAAAGGGAGAAACTGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTAAATGCCTATCATCAGCGAAATGAAGTGACAATAGCGGCTCGTTTAATTGAAGCTAAAATTCC
TTCTATTGAAGCACTTAATATGCCACAAGTGTTATATAAGCTTGCGGAAAAACCACAAGGTTTAATTTTAGTAACTGGTC
CAACAGGCTCAGGTAAATCAACTACATTAGCAGCAATGATTGATTATATCAACGAGACAAGATCAAAACATATTATTACG
TTAGAGGATCCAATTGAATACTTGCATACTCATAAAAAGTCTATCATCAATCAGCGTGAGGTTGGTGTCGATACAATGAG
TTTTGCGAATGGTTTACGAGCATCATTGCGTCAAGACCCAGATATTATTTTAGTTGGGGAGATGCGAGATTATGAAACGA
TTTCAACGGCTATCACGGCTGCTGAAACAGGGCATTTAGTAATGGCAACACTTCATACAAGTAGTGCACCAACTACAATC
GACCGTATTATTGATGTATTTCCACCGCATCAGCAAAGTCAAATTCGAGTACAGCTTGCGAATGTACTGCAAGGTATTGT
TTCACAGCGTTTATTTATTCGTAAAGATGTAAAAGGGCGCATTGCTGCGACAGAAATTTTAGTACAAGCTCCTGCCATTT
CGAATTTAATTCGCAATGAAAAAATTCACCAAATTCCAAGCGTAATGCAGACAAGTAAAGCATTGGGCATGCACACATTA
GAATCTTCGATGCAGCACCTGATTACCACAGGTAAGATCTCGCTAGAAGATGCACGGCCATACATGAACGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.959

97.126

0.514
  pilT Neisseria meningitidis 8013

50.599

95.977

0.486
  pilT Neisseria gonorrhoeae MS11

50.299

95.977

0.483
  pilT Pseudomonas aeruginosa PAK

50

95.977

0.48
  pilT Acinetobacter baumannii D1279779

49.701

95.977

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.701

95.977

0.477
  pilT Acinetobacter nosocomialis M2

49.701

95.977

0.477
  pilT Acinetobacter baumannii strain A118

49.701

95.977

0.477
  pilT Acinetobacter baylyi ADP1

49.401

95.977

0.474
  pilT Vibrio cholerae strain A1552

49.102

95.977

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.102

95.977

0.471
  pilT Legionella pneumophila strain ERS1305867

49.544

94.54

0.468
  pilT Legionella pneumophila strain Lp02

49.544

94.54

0.468
  pilU Pseudomonas stutzeri DSM 10701

43.505

95.115

0.414
  pilU Vibrio cholerae strain A1552

43.168

92.529

0.399
  pilU Acinetobacter baylyi ADP1

40.237

97.126

0.391

Multiple sequence alignment