Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKY25_RS05280 Genome accession   NZ_CP151952
Coordinates   1070551..1071606 (+) Length   351 a.a.
NCBI ID   WP_339232795.1    Uniprot ID   -
Organism   Geobacillus sp. FSL W8-0032     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1065551..1076606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY25_RS05265 (MKY25_05265) - 1066749..1067525 (+) 777 WP_061912467.1 PRC-barrel domain-containing protein -
  MKY25_RS05270 (MKY25_05270) - 1067543..1068877 (+) 1335 WP_033844231.1 G5 domain-containing protein -
  MKY25_RS05275 (MKY25_05275) - 1068874..1070538 (+) 1665 WP_033844230.1 ATPase, T2SS/T4P/T4SS family -
  MKY25_RS05280 (MKY25_05280) pilT 1070551..1071606 (+) 1056 WP_339232795.1 type IV pilus twitching motility protein PilT Machinery gene
  MKY25_RS05285 (MKY25_05285) - 1071593..1072804 (+) 1212 WP_033844228.1 type II secretion system F family protein -
  MKY25_RS05290 (MKY25_05290) - 1072898..1073359 (+) 462 WP_033844227.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MKY25_RS05295 (MKY25_05295) - 1073378..1074133 (+) 756 WP_033844226.1 A24 family peptidase -
  MKY25_RS05300 (MKY25_05300) pilM 1074163..1075098 (+) 936 WP_033844225.1 pilus assembly protein PilM -
  MKY25_RS05305 (MKY25_05305) - 1075088..1075657 (+) 570 WP_033844224.1 anti sigma-E factor RseA C-terminal domain-containing protein -
  MKY25_RS05310 (MKY25_05310) - 1075654..1076364 (+) 711 WP_061912357.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38628.70 Da        Isoelectric Point: 7.5558

>NTDB_id=981903 MKY25_RS05280 WP_339232795.1 1070551..1071606(+) (pilT) [Geobacillus sp. FSL W8-0032]
MKEKLDTLLRAAFEYRASDVHLTVGAPPIFRLNGELRLYGQERLRPEETEGMARAVIPEALWQQFQQTGELDFSYGIAGV
SRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPSVLKRIAEKPHGLVLVTGPTGSGKSTTLTAMIDYINKTMSKHIITL
EDPIEYLHKHGKSIIDQREVGFDTGSFASGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPSAQQAQIRLQLASVLVAIIAQRLFPNRSRTGRAAALEILLNNAAVANLIRNEKEHQIINIMQTSRSVGMQTME
MSVKELVAAGRIDAAAVEVYLAGEREKDGAI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=981903 MKY25_RS05280 WP_339232795.1 1070551..1071606(+) (pilT) [Geobacillus sp. FSL W8-0032]
ATGAAAGAAAAATTGGATACGTTGTTGCGCGCGGCGTTTGAGTACCGGGCGTCCGACGTGCATTTGACCGTCGGAGCGCC
GCCGATTTTTCGCCTGAACGGCGAGCTGAGGCTCTACGGCCAAGAGCGGCTGCGCCCTGAGGAAACGGAAGGAATGGCGC
GCGCCGTCATCCCTGAAGCGCTTTGGCAGCAGTTCCAACAAACCGGGGAGCTTGATTTTTCCTATGGGATCGCCGGCGTG
TCCCGGTTCCGCTTGAACGTGTTTAAACAGCGGTCCTGTGTTTCCTTGGCCGTTCGCCTCATCCCGATGCGCATCCCGAC
GTTGGAGGAGCTTGGCATGCCAAGCGTGCTGAAGCGCATCGCGGAAAAGCCGCACGGGCTTGTGCTCGTCACCGGGCCGA
CCGGCAGTGGCAAGTCGACGACGTTGACCGCTATGATCGATTATATAAACAAAACGATGTCGAAGCATATTATTACGCTG
GAGGATCCGATCGAATATTTACATAAACACGGGAAATCGATCATTGATCAACGCGAGGTCGGATTTGACACCGGCAGCTT
TGCTTCCGGGCTGCGCGCCGCGCTTCGCCAAGACCCGGACGTCATTTTAGTCGGGGAGATGCGCGACTTGGAGACGATTC
AAACTGCCATTACGGCCGCGGAAACGGGGCATTTGGTGTTGGGCACGCTCCATACGGCCAGCGCCCCGGCGGCGATCGAG
CGGATGATTGACGTCTTTCCTTCGGCTCAGCAGGCGCAAATCCGCTTGCAGCTCGCTTCAGTGCTTGTCGCCATTATTGC
GCAGCGCCTGTTTCCGAACCGAAGCCGAACCGGACGGGCGGCGGCGCTCGAAATTTTACTGAACAATGCCGCGGTCGCCA
ATCTCATCCGCAACGAGAAGGAACACCAAATCATCAACATTATGCAGACGAGCCGCAGTGTGGGCATGCAGACGATGGAA
ATGAGCGTCAAGGAACTGGTTGCGGCCGGGCGGATTGATGCGGCGGCAGTTGAAGTGTACTTAGCGGGGGAGCGTGAGAA
AGATGGCGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(96-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.994

89.174

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.943

94.302

0.462
  pilT Vibrio cholerae strain A1552

48.943

94.302

0.462
  pilT Neisseria meningitidis 8013

48.328

93.732

0.453
  pilT Neisseria gonorrhoeae MS11

48.024

93.732

0.45
  pilT Pseudomonas aeruginosa PAK

49.051

90.028

0.442
  pilT Acinetobacter baylyi ADP1

47.239

92.877

0.439
  pilT Acinetobacter baumannii D1279779

46.769

92.593

0.433
  pilT Acinetobacter baumannii strain A118

46.769

92.593

0.433
  pilT Acinetobacter nosocomialis M2

46.462

92.593

0.43
  pilT Pseudomonas stutzeri DSM 10701

47.468

90.028

0.427
  pilT Legionella pneumophila strain Lp02

46.562

91.168

0.424
  pilT Legionella pneumophila strain ERS1305867

46.562

91.168

0.424
  pilU Vibrio cholerae strain A1552

42.553

93.732

0.399
  pilU Pseudomonas stutzeri DSM 10701

42.249

93.732

0.396

Multiple sequence alignment